Fermented lingonberry juice's effects on active MMP‐8 (aMMP‐8), bleeding on probing (BOP), and visible plaque index (VPI) in dental implants—A clinical pilot mouthwash study

Lähteenmäki, Hanna; Tervahartiala, Taina; Räisänen, Ismo T.; Pärnänen, Pirjo; Sorsa, Timo

doi:10.1002/cre2.638

Cited by 5 publications

(2 citation statements)

References 45 publications

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“…However, the expectations were not met in a pilot study on participants with dental implants: the difference between groups with and without FLJ mouthwash twice daily for 15 days diminished after the FLJ regimen had ended. Remarkedly, the decrease in aMMP-8 (active matrix metalloproteinase) levels appeared to continue [20]. This key biomarker for periodontitis and peri-implantitis had a sensitivity of 75-85% and a specificity in the range of 80-90% [21].…”

Section: Discussionmentioning

confidence: 91%

In Vitro Eradication of Planktonic, Saliva and Biofilm Bacteria Using Lingonberry Extract as a Photosensitizer for Visible Light Plus Water-Filtered Infrared-A Irradiation

Klein,

Al-Ahmad,

Follo

et al. 2023

Nutrients

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Antimicrobial photodynamic treatment (aPDT) with visible light plus water-filtered infrared-A irradiation (VIS-wIRA) and natural single- or multi-component photosensitizers (PSs) was shown to have potent antimicrobial activity. The aim of this study was to obtain information on the antimicrobial effects of aPDT-VIS-wIRA with lingonberry extract (LE) against bacteria that play a role in oral health. Planktonic bacterial cultures of the Gram-positive E. faecalis T9, S. mutans DSM20523, S. oralis ATCC 35037 and S. sobrinus PSM 203513, the Gram-negative N. oralis 14F2 FG-15-7B, F. nucleatum ATCC 25586, and V. parvula DSM, the anaerobic F. nucleatum ATCC 25586 and V. parvula DSM 2008, and the total mixed bacteria from pooled saliva and supra- and subgingival plaques of volunteers were all treated and compared. aPDT-VIS-wIRA with LE as PS significantly (p < 0.008) reduced the growth of all tested Gram-positive, Gram-negative, as well as aerobic and anaerobic bacterial strains, whereas without irradiation no reductions were seen (p < 0.0001). NaCl, with or without irradiation, was ineffective. After treatment with CHX 0.2%, the highest killing rate (100%) was observed, and no bacteria (0 log10 CFU) were cultivable. The method also significantly reduced all of the bacteria present in saliva and in the gingival biofilms. Three-dimensional visualization of viable and non-viable microorganisms revealed that LE penetrated deeper into the cell wall layers than CHX 0.2%. LE was an appropriate PS for eradicating microorganisms with VIS-wIRA, either in their planktonic form or in saliva and gingival plaque biofilms. These results encourage further investigation in order to determine which LE compounds contribute to the photosensitizing effect and to evaluate the size of the effect on maintaining oral health.

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Section: Discussionmentioning

confidence: 91%

In Vitro Eradication of Planktonic, Saliva and Biofilm Bacteria Using Lingonberry Extract as a Photosensitizer for Visible Light Plus Water-Filtered Infrared-A Irradiation

Klein,

Al-Ahmad,

Follo

et al. 2023

Nutrients

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“…36 Clinical human trials have shown beneficial effects of FLJ on salivary parameters and reducing xerostomia, 37 and potential inhibition of plaque levels, bleeding on probing, and inflammation in dental implants. 38…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial and Anti-Inflammatory Oral Effects of Fermented Lingonberry Juice—A One-Year Prospective Human Intervention Study

et al. 2023

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Objectives A 1-year prospective human intervention study was performed to examine the anticaries, anti-inflammatory, antiproteolytic, and antimicrobial effects of fermented lingonberry juice (FLJ), used as a mouthwash for a period of 6 months, followed by a 6-month washout period. Materials and Methods Twenty-five adults were recruited from private dental clinics in Helsinki and Joensuu (Finland). Standard oral examinations and sample gatherings were performed at base level, 6 months, and 1 year for oral Streptococcus mutans (S. mutans), Candida, and Lactobacilli levels, and active matrix metalloprotease-8 (aMMP-8) levels, and for decayed, missing, filled teeth (DMFT), decayed, missing filled surfaces (DMFS) and decayed surfaces (DS) indexes, and probing pocket depths (PPDs), bleeding on probing (BOP), and visible plaque index (VPI). FLJ was used by the participants once daily for 30 seconds for 6 months. FLJ contains 0.212% (w/v) polyphenols, 3% (w/ v) sugars, and contains no excipients. Ten milliliters of FLJ were equal to 1 dL of lingonberry juice. Statistical Analysis Statistical analyses were performed with nonparametric Friedman's test and pairwise post-hoc analysis with Dunn-Bonferroni test, SPSS (version 27; IBM) and p < 0.05 was considered as statistically significant. Results The levels of S. mutans and Candida counts, DS, BOP, and VPI decreased significantly (p < 0.05) during the FLJ period. Lactobacilli counts increased significantly, while there was also significant difference in aMMP-8 levels, DMFT, and DMFS between the three measurement points. PPDs were not affected. Conclusions The specially formulated FLJ may have a positive decreasing effect on S. mutans, and Candida counts as well as decrease low-grade inflammation and proteolytic burden in the oral mucosa and periodontal tissues. The beneficial effects to the oral cavity of FLJ mouthwash may be useful among patients with oral diseases, such as dental caries, periodontitis and candidosis.

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Fermented lingonberry juice's effects on active MMP‐8 (aMMP‐8), bleeding on probing (BOP), and visible plaque index (VPI) in dental implants—A clinical pilot mouthwash study

Lähteenmäki

Tervahartiala

Räisänen

et al. 2022

Clinical & Exp Dental Res

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Objectives We aimed to study the effects of fermented lingonberry juice (FLJ) as a mouthwash on the levels of active matrix metalloproteinase‐8 (aMMP‐8) in peri‐implant sulcular fluid (PISF), bleeding on probing (BOP), and visible plaque index (VPI). We hypothesized that FLJ rinsing could reduce inflammation (aMMP‐8 and BOP) and microbial load (VPI) in the oral cavity, especially around dental implants. Materials and Methods A clinical pilot study was performed using FLJ as a mouthwash. The inclusion criteria were at least one dental implant in the anterior or posterior areas with a screw‐retained crown. Ten participants used 10 ml of mouthwash twice a day for 15 days, and 10 participants served as the control group. Point‐of‐care tests (POCTs) were used to measure aMMP‐8 levels in the PISF, and BOP and VPI were recorded at the beginning of the trial and after 15 and 30 days. Results The FLJ mouthwash had a reductive effect on aMMP‐8, VPI, and BOP in the mouthwash group; however, there was no significant difference compared to the control group. The difference in VPI and BOP levels between the groups diminished after the lingonberry regimen ended. The decrease in aMMP‐8 levels appeared to continue even after discontinuation of the mouthwash regimen. Conclusion The reduction in the amount of plaque, aMMP‐8, and BOP by FLJ was promising and continuous considering the relatively short study period and sample size. FLJ is a natural and safe supplement for oral and dental implant home care. Further studies are required to verify these promising results.

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Fermented lingonberry juice's effects on active MMP‐8 (aMMP‐8), bleeding on probing (BOP), and visible plaque index (VPI) in dental implants—A clinical pilot mouthwash study

Cited by 5 publications

References 45 publications

In Vitro Eradication of Planktonic, Saliva and Biofilm Bacteria Using Lingonberry Extract as a Photosensitizer for Visible Light Plus Water-Filtered Infrared-A Irradiation

In Vitro Eradication of Planktonic, Saliva and Biofilm Bacteria Using Lingonberry Extract as a Photosensitizer for Visible Light Plus Water-Filtered Infrared-A Irradiation

Antimicrobial and Anti-Inflammatory Oral Effects of Fermented Lingonberry Juice—A One-Year Prospective Human Intervention Study

Fermented lingonberry juice's effects on active MMP‐8 (aMMP‐8), bleeding on probing (BOP), and visible plaque index (VPI) in dental implants—A clinical pilot mouthwash study

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