Fermented egg-milk beverage alleviates dextran sulfate sodium-induced colitis in mice through the modulation of intestinal flora and short-chain fatty acids

Lyu, Siwen; Pan, Fengguang; Ge, Huifang; Yang, Qi; Duan, Xuehui; Feng, Mengmeng; Liu, Xuanting; Zhang, Biying; Liu, Jingbo

doi:10.1039/d1fo03040j

Cited by 12 publications

(15 citation statements)

References 42 publications

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“…Bioactive peptides derived from the raw material or other food ingredients were reported as having intestinal protective functions and features. Our previous research found that fermented egg-milk peptides could alleviate intestinal inflammation symptoms and establish the gut microbiota in colitis mice ( 15 ). However, the protective function of the damaged intestinal tissue barrier and the underlying biological mechanism are not clear now.…”

Section: Discussionmentioning

confidence: 99%

“…The ExPASy PeptideCutter program, a database available at https://web.expasy.org/peptide_cutter/ , was used here to collect the FEMPs' information. All the proteins and peptides in fermented egg milk and the sequences of LC-MS/MS results were collected based on our previous study ( 15 ) and uploaded into the PeptideCutter database. Pepsin and trypsin were selected as the digestive enzymes to perform the digestion process.…”

Section: Methodsmentioning

confidence: 99%

“…inflammation symptoms and establish the gut microbiota in colitis mice (15). However, the protective function of the damaged intestinal tissue barrier and the underlying biological mechanism are not clear now.…”

Section: Figurementioning

confidence: 99%

“…The results confirmed that egg white peptides could repair the mucosa and intestinal structure of the damaged tissue ( 14 ). Our previous research also investigated the protective function of fermented egg milk on colitis induced by dextran sulfate sodium (DSS) in mice ( 15 ). However, the underlying mechanism and potential targets of the fermented egg-milk peptides' (FEMPs) action on the damaged intestinal barrier need further research.…”

Section: Introductionmentioning

confidence: 99%

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Protective effects and potential mechanisms of fermented egg-milk peptides on the damaged intestinal barrier

et al. 2022

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IntroductionFermented egg-milk peptides (FEMPs) could enhance the colon-intestinal barrier and upgrade the expression of zonula occludens-1 and mucin 2. Besides, the underlying biological mechanism and the targets FEMPs could regulate were analyzed in our study.MethodsHerein, the immunofluorescence technique and western blot were utilized to evaluate the repair of the intestinal barrier. Network pharmacology analysis and bioinformatics methods were performed to investigate the targets and pathways affected by FEMPs.Results and discussionAnimal experiments showed that FEMPs could restore intestinal damage and enhance the expression of two key proteins. The pharmacological results revealed that FEMPs could regulate targets related to kinase activity, such as AKT, CASP, RAF, and GSK. The above targets could interact with each other. GO analysis indicated that the targets regulated by FEMPs could participate in the kinase activity of the metabolic process. KEGG enrichment revealed that the core targets were enriched in pathways related to cell apoptosis and other important procedures. Molecular docking demonstrated that FEMPs could bind to the key target AKT via hydrogen bond interactions. Our study combined the experiment in vivo with the method in silico and investigated the interaction between peptides and targets in a pattern of multi-targets and multi-pathways, which offered a new perspective on the functional validation and potential application of bioactive peptides.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Protective effects and potential mechanisms of fermented egg-milk peptides on the damaged intestinal barrier

et al. 2022

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“…A recent study reported that egg consumption improved gut microbiota [140]. Fermented egg-milk beverage also increased beneficial bacteria from Lachnospiraceae and Lactobacillus [141]. Ovomucin has been reported to improve gut microbiota through inhibiting pathogenic bacteria [142].…”

Section: Eggsmentioning

confidence: 99%

The Role of the Gut-Lung Axis in COVID-19 Infections and Its Modulation to Improve Clinical Outcomes

Chen¹,

Vitetta²

2022

Front. Biosci. (Schol Ed)

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The main entry point of SARS-CoV-2 is the respiratory tract and as such immune defence in this site determines if the virus will spillover to the systemic circulation and circulate and infect other major organs. The first line of mucosal immune defence is composed of mucins, an epithelial barrier, and immune cells in the nasal cavity. The lung immune defence is carried out by numerous alveoli. The lung microbiota is a key factor in determining the efficacy of lung mucosal immunity protection. The intestinal microbiota has been demonstrated to affect the severity of COVID-19. Gut dysbiosis is involved in hyperinflammation and multiple organ failure through communications with multiple organs. The gut lung axis could be the earliest axis affected in COVID-19. Through the gut-lung axis, gut dysbiosis can affect the pathogenesis of the lung in COVID-19. In this review, we summarise the effects that gut dysbiosis can progress on the lung, and the lung microbiota. The possible mechanisms and approaches for modulation are discussed.

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The effects of microbiota-targeted approaches in inflammatory bowel disease: probiotics, probiotic foods, and prebiotics

Xia

Liu²,

Li³

et al. 2023

Current Opinion in Food Science

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Fermented egg-milk beverage alleviates dextran sulfate sodium-induced colitis in mice through the modulation of intestinal flora and short-chain fatty acids

Abstract: Fermented egg milk beverage (FEMB) can alleviate the symptoms of intestinal diseases by regulating intestinal flora and supplying nutrition. This study was investigated in the protective effect of fermented egg...

Cited by 12 publications

References 42 publications

Protective effects and potential mechanisms of fermented egg-milk peptides on the damaged intestinal barrier

Protective effects and potential mechanisms of fermented egg-milk peptides on the damaged intestinal barrier

The Role of the Gut-Lung Axis in COVID-19 Infections and Its Modulation to Improve Clinical Outcomes

The effects of microbiota-targeted approaches in inflammatory bowel disease: probiotics, probiotic foods, and prebiotics

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