Fermentative Production of Nervonic Acid by Mortierella capitata RD000969

Umemoto, Hiroki; Sawada, Kaichiro; Kurata, Atsushi; Hamaguchi, Shohei; Tsukahara, Satoshi; Ishiguro, Takashi; Kishimoto, Noriaki

doi:10.5650/jos.ess14029

Cited by 20 publications

(15 citation statements)

References 23 publications

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“…Nervonic acid can only be incorporated into the sn-1 or sn-3 position of TAGs through endogenous acyltransferases, but not into both positions at the same time, and is predicted more likely to be incorporated into sn-3. However, C16 and C18 FA are typically found instead of NA in the sn-2 position in the fungus Mortierella capitata RD000969, microalga Mychonastes afer (Umemoto et al, 2014;Fan et al, 2018b). Similar limitation of further increase NA content also occurs in high-EA B. napus and B. carinata and negligible NA is found at the sn-2 position on the glycerol backbone because the ability of endogenous Brassica LPAAT to incorporate nervonoyl moieties into sn-2 position is very low (Scarth and Tang, 2006;Taylor et al, 2011).…”

Section: Other Optimal Participants In Vlcfa Elongation Are Availablementioning

confidence: 95%

“…The third acylation reaction, converting DAG to TAG, is catalyzed by diacylglycerol acyltransferase (DGAT) enzymes at the sn-3 position using a fatty acyl-CoA (Figure 2) (Kennedy and Weiss, 1956). The research findings showed that NA can only be incorporated into the sn-1 or sn-3 position instead of sn-2 position of TAGs (Scarth and Tang, 2006;Taylor et al, 2011;Umemoto et al, 2014;Fan et al, 2018b).…”

Section: Na Biosynthesis and Assemblymentioning

confidence: 99%

“…However, long growth cycles, limited distributions, and highly variable seed yields have hindered the extraction of NA from natural plant sources (Tang et al, 2013). The construction of NA-producing microorganisms, microalgae, and plants via genetic engineering for nutraceutical and pharmacological applications offers a suitable approach that has attracted widespread attention due to its potential application in the large-scale production of NA (Guo et al, 2009;Taylor et al, 2009;Yuan et al, 2011;Umemoto et al, 2014;Huai et al, 2015;Fillet et al, 2017;Fan et al, 2018a;Xu et al, 2018;Liu et al, 2020). Recently, reviews on the genetic engineering of microorganisms and microalgae for the production of NA have been extensively conducted (Fan et al, 2018a;.…”

Section: Introductionmentioning

confidence: 99%

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A Review of Nervonic Acid Production in Plants: Prospects for the Genetic Engineering of High Nervonic Acid Cultivars Plants

et al. 2021

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Nervonic acid (NA) is a very-long-chain monounsaturated fatty acid that plays crucial roles in brain development and has attracted widespread research interest. The markets encouraged the development of a refined, NA-enriched plant oil as feedstocks for the needed further studies of NA biological functions to the end commercial application. Plant seed oils offer a renewable and environmentally friendly source of NA, but their industrial production is presently hindered by various factors. This review focuses on the NA biosynthesis and assembly, NA resources from plants, and the genetic engineering of NA biosynthesis in oil crops, discusses the factors that affect NA production in genetically engineered oil crops, and provides prospects for the application of NA and prospective trends in the engineering of NA. This review emphasizes the progress made toward various NA-related topics and explores the limitations and trends, thereby providing integrated and comprehensive insight into the nature of NA production mechanisms during genetic engineering. Furthermore, this report supports further work involving the manipulation of NA production through transgenic technologies and molecular breeding for the enhancement of crop nutritional quality or creation of plant biochemical factories to produce NA for use in nutraceutical, pharmaceutical, and chemical industries.

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Section: Other Optimal Participants In Vlcfa Elongation Are Availablementioning

confidence: 95%

Section: Na Biosynthesis and Assemblymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Review of Nervonic Acid Production in Plants: Prospects for the Genetic Engineering of High Nervonic Acid Cultivars Plants

et al. 2021

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“…NA has been identified in several seed oils, including those of Lunaria annua and Acer truncatum. In a previous study, we reported a method for the production of NArich, single-cell oil fermented by a filamentous fungus, Mortierella capitata RD000969 18 . NA is a major fatty acid present in the sphingomyelin of myelin sheath and plays an essential role in signal transmission in the CNS.…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Nervonic Acid Against 6-Hydroxydopamine-Induced Oxidative Stress in PC-12 Cells

et al. 2021

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“…As a natural component of human breast milk, nervonic acid is currently being used for infant formula supplementation [ 9 – 11 ]. As raw materials for industrial, pharmaceutical and nutraceutical applications, effective production of VLCFAs in oilseed, especially nervonic acid, has become an important goal of oilseed crop breeding [ 12 – 15 ].…”

Section: Introductionmentioning

confidence: 99%

Combinatorial Effects of Fatty Acid Elongase Enzymes on Nervonic Acid Production in Camelina sativa

Huai

Zhang

et al. 2015

PLoS ONE

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Very long chain fatty acids (VLCFAs) with chain lengths of 20 carbons and longer provide feedstocks for various applications; therefore, improvement of VLCFA contents in seeds has become an important goal for oilseed enhancement. VLCFA biosynthesis is controlled by a multi-enzyme protein complex referred to as fatty acid elongase, which is composed of β-ketoacyl-CoA synthase (KCS), β-ketoacyl-CoA reductase (KCR), β-hydroxyacyl-CoA dehydratase (HCD) and enoyl reductase (ECR). KCS has been identified as the rate-limiting enzyme, but little is known about the involvement of other three enzymes in VLCFA production. Here, the combinatorial effects of fatty acid elongase enzymes on VLCFA production were assessed by evaluating the changes in nervonic acid content. A KCS gene from Lunaria annua (LaKCS) and the other three elongase genes from Arabidopsis thaliana were used for the assessment. Five seed-specific expressing constructs, including LaKCS alone, LaKCS with AtKCR, LaKCS with AtHCD, LaKCS with AtECR, and LaKCS with AtKCR and AtHCD, were transformed into Camelina sativa. The nervonic acid content in seed oil increased from null in wild type camelina to 6-12% in LaKCS-expressing lines. However, compared with that from the LaKCS-expressing lines, nervonic acid content in mature seeds from the co-expressing lines with one or two extra elongase genes did not show further increases. Nervonic acid content from LaKCS, AtKCR and AtHCD co-expressing line was significantly higher than that in LaKCS-expressing line during early seed development stage, while the ultimate nervonic acid content was not significantly altered. The results from this study thus provide useful information for future engineering of oilseed crops for higher VLCFA production.

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Fermentative Production of Nervonic Acid by Mortierella capitata RD000969

Cited by 20 publications

References 23 publications

A Review of Nervonic Acid Production in Plants: Prospects for the Genetic Engineering of High Nervonic Acid Cultivars Plants

A Review of Nervonic Acid Production in Plants: Prospects for the Genetic Engineering of High Nervonic Acid Cultivars Plants

Protective Effect of Nervonic Acid Against 6-Hydroxydopamine-Induced Oxidative Stress in PC-12 Cells

Combinatorial Effects of Fatty Acid Elongase Enzymes on Nervonic Acid Production in Camelina sativa

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