Fermentation of sweet whey by recombinant Escherichia coli K011

Leite, Amarildo Ricardo; Guimarães, W. V.; Araújo, Elza Fernandes de; Silva, Daison Olzany

doi:10.1590/s1517-83822000000300011

Cited by 32 publications

(16 citation statements)

References 13 publications

(23 reference statements)

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“…However, at 55 C, elution of b-lactoglobulin from the ion exchanger was difficult (32). Therefore, taking the effects of temperature on b-lactoglobulin (4,5,31,33), protease N Amano and DEAE sepharose 1 (31) into consideration, 45 C was chosen as the optimum temperature for further experiments.…”

Section: Hydrolysis Of B-lactoglobulin By Protease N Amanomentioning

confidence: 99%

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Development of an Integrative Process for the Production of Bioactive Peptides from Whey by Proteolytic Commercial Mixtures

Welderufael

Jauregi

2010

Separation Science and Technology

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The aim of this work is to develop an integrative process based on the use of an ion exchange resin for the enzymatic hydrolysis of b-lactoglobulin to produce peptides with angiotensin converting enzyme (ACE) inhibitory activity using whey as a feed stock. Unlike the conventional methods, the main advantage of this approach is that by integrating the selective separation of b-lactoglobulin from whey and its hydrolysis less complex mixtures of peptides are produced. Furthermore, peptides of similar charge as b-lactoglobulin remain adsorbed achieving further purification. In this work, the enzyme protease N Amano at an enzyme to substrate ratio of 1/100 (wt/wt) was added directly to the adsorbed proteins in a thermostatically controlled membrane reactor operated in batch mode. Separation of the smaller peptides from the enzyme and larger peptides was achieved with a 1 kDa molecular weight cut-off ultrafiltration membrane. Also, this step enables the recycling of nonhydrolyzed substrates, large peptides, and enzyme. The adsorbed protein was re-solubilised in a 10 mM potassium phosphate buffer (pH 7 and 45 C). The different fractions were assayed for their bioactivity in terms of angiotensin converting enzyme inhibition percentage (ACEi%) and IC 50 which is the concentration of peptides that can inhibit the ACE activity by 50%. Results show that permeates of 2 and 6 hrs hydrolysis have the highest bioactivity with IC 50 ¼ 67 and 98 lg=ml respectively.

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Section: Hydrolysis Of B-lactoglobulin By Protease N Amanomentioning

confidence: 99%

“…A vast amount of whey is produced worldwide annually and estimated about 115 million metric tons. Out of this only 53% are used as whey products in food and the rest of the 47% are dumped as a waste into the environment (2)(3)(4). This vast amount of waste product consists of abundant valuable proteins (see Table 1).…”

Section: Introductionmentioning

confidence: 99%

Development of an Integrative Process for the Production of Bioactive Peptides from Whey by Proteolytic Commercial Mixtures

Welderufael

Jauregi

2010

Separation Science and Technology

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“…Molasses, a residue of the sugar industry, is also used in Brazil for ethanol fermentation (31). Other sugars substrates, such as lactose from cheese whey (1,9,14,21) and pentoses and hexoses from lignocellulosic biomass, can be converted to ethanol (1,2,3,12,17,23,28,32). The yeast S. cerevisiae and the bacterium Zymomonas mobilis are highly efficient in alcohol fermentation but they can not to use many source of sugars substrates (7).…”

Section: Introductionmentioning

confidence: 99%

“…The efficiency of KO11 and P2 in conversion of various substrates to ethanol, including simple sugars (5,16,21,22,29), starch (15), and hydrolyzed lignocellulose (2,3,11,12,13) has been determined. In this study we examined growth and ethanol fermentation of sucrose, sugarcane juice and molasses by these recombinants.…”

Section: Introductionmentioning

confidence: 99%

Ethanolic fermentation of sucrose, sugarcane juice and molasses by Escherichia coli strain ko11 and Klebsiella oxytoca strain P2

Silva¹,

Araújo²,

Silva³

et al. 2005

Braz. J. Microbiol.

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Escherichia coli KO11 and Klebsiella oxytoca P2 recombinants fermented sucrose to ethanol. In minimal medium with 2% or 12% added sucrose KO11 produced 75% and 41%, respectively, of the maximum theoretical yield (0.54g ethanol/g sucrose). In Luria-Bertani (LB) broth with up to 8% sucrose, KO11 presented a 94-96% yield and with 12% sucrose, KO11 presented about 69% yield (44.5g ethanol/L). P2 presented 55% of the theoretical maximum yield in minimal medium supplemented with 2% sucrose and 47% of the maximum in 12% sucrose. In LB broth with 2 or 4% sucrose, P2 presented 94-95% of the theoretical maximum yield, which fell to 73% with 8% added sucrose (31.4g ethanol/L) and 58% with 12% sucrose (37.5 g/L). Volumetric productivity in LB broth containing 2% sucrose was 0.41 g/L/h for KO11 and 1.1 g/L/h for P2, while in LB broth with 12% added sucrose, productivity was 0.96 g/L/h (KO11) and 1.4 g/L/h (P2). During fermentation of sugar cane juice by E. coli KO11 and K. oxytoca P2 produced 39.4 g/L and 42.1 g/L ethanol when supplemented with 0.5% yeast extract, micronutrients and thiamine. In sugar cane juice supplemented with LB broth ingredients, KO11 ethanol fermentation was inhibited with production of only 23.0 g ethanol/L, while P2 produced 44.2 g/ L. Ethanol production by KO11 and P2, respectively, in sugarcane juice was a) 25.3 and 30.2 g/L with 0.2% ammonium sulfate, b) 24.9 and 31.6 g/L with ammonium sulfate and micronutrients, c) 25.6 and 37.5 g/L with ammonium sulfate, micronutrients and thiamine. During molasses fermentation E. coli KO11 presented low ethanol production and high lactic acid production. K. oxytoca P2 produced 25 g ethanol/L in molasses diluted 10-fold in water, with or without addition of 0.5% yeast extract, and 27.8 g/L with addition of LB broth ingredients after 96h. P2 produced 24.5, 26.9, and 28.0 g ethanol/L in molasses diluted 10-fold in vinasse, vinasse with 0.5% added yeast extract and with LB broth ingredients, respectively.

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“…coli strain KO11 and derivatives have been used for laboratory production of ethanol from many types of plant biomass including rice hulls (Moniruzzaman and Ingram, 1998), sugar cane bagasse, agricultural residues (Asghari et al, 1996), corn cobs, hulls and ammonia fiber explosion (AFEX)-pre-treated fibres (Beall et al, 1992;Lau et al, 2007;Moniruzzaman et al, 1996), orange peel (Grohmann et al, 1994), pectin-rich beet pulp (Doran et al, 2000), sweet whey (Leite et al, 2000), brewery waste (Rao et al, 2007), cotton gin waste (Jeoh and Agblevor, 2001) and waste house wood (Okuda et al, 2007), in addition to several other sources. Ethanol production and yield on a grams of total ethanol per gram of sugar basis is presented in Table 3.…”

Section: Fermentations Using E Coli Strains Ko11 and Ly01mentioning

confidence: 99%

Microbial conversion of sugars from plant biomass to lactic acid or ethanol

2008

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SummaryConcerns for our environment and unease with our dependence on foreign oil have renewed interest in converting plant biomass into fuels and 'green' chemicals. The volume of plant matter available makes lignocellulose conversion desirable, although no single isolated organism has been shown to depolymerize lignocellulose and efficiently metabolize the resulting sugars into a specific product. This work reviews selected chemicals and fuels that can be produced from microbial fermentation of plant-derived cell-wall sugars and directed engineering for improvement of microbial biocatalysts. Lactic acid and ethanol production are highlighted, with a focus on engineered Escherichia coli.

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Fermentation of sweet whey by recombinant Escherichia coli K011

Cited by 32 publications

References 13 publications

Development of an Integrative Process for the Production of Bioactive Peptides from Whey by Proteolytic Commercial Mixtures

Development of an Integrative Process for the Production of Bioactive Peptides from Whey by Proteolytic Commercial Mixtures

Ethanolic fermentation of sucrose, sugarcane juice and molasses by Escherichia coli strain ko11 and Klebsiella oxytoca strain P2

Microbial conversion of sugars from plant biomass to lactic acid or ethanol

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