Fermentation of<i>Bacillus</i>

Arbige, Michael V.; Bulthuis, Ben A.; Schultz, J; Crabb, Douglas W

doi:10.1128/9781555818388.ch60

Cited by 11 publications

(1 citation statement)

References 149 publications

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“…Genus Bacillus is a Gram-positive, spore-forming, fermentative, aerobic and rodshaped bacteria. Several species of this group are non-pathogenic, simple to cultivate and secrete enzymes such as proteases, amylases and cellulases that are useful for a number of industrial applications (Arbige et al 1993 ). The Bacillus subtilis group contains the closely related taxa Bacillus subtilis subsp.…”

Section: Introductionmentioning

confidence: 99%

Specific oligonucleotide primers for detection of endoglucanase positive Bacillus subtilis by PCR

et al. 2013

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A polymerase chain reaction (PCR) assay was developed for discrimination of Bacillus subtilis from other members of B. subtilis group as well as rapid identification from environmental samples. Primers ENIF and EN1R from endoglucanase gene were used to amplify a1311 bp DNA fragment. The specificity of the primers was tested with seven reference strains and 28 locally isolated strains of endoglucanase positive Bacillus species. The PCR product was only produced from B. subtilis. The results demonstrated high specificity of two oligonucleotides for B. subtilis. This species-specific PCR method provides a quick, simple, powerful and reliable alternative to conventional methods in the detection and identification of B. subtilis. To our knowledge this is the first report of a B. subtilis specific primer set.

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Section: Introductionmentioning

confidence: 99%

Specific oligonucleotide primers for detection of endoglucanase positive Bacillus subtilis by PCR

et al. 2013

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Metabolic capacity ofBacillus subtilis for the production of purine nucleosides, riboflavin, and folic acid

Sauer

Cameron

Bailey

1998

Biotechnol. Bioeng.

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We developed a stoichiometric model of Bacillus subtilis metabolism for quantitative analysis of theoretical growth and biochemicals production capacity. This work concentrated on biochemicals that are derived from the purine biosynthesis pathway; inosine, guanosine, riboflavin, and folic acid. These are examples of commercially relevant biochemicals for which Bacillus species are commonly used production hosts. Two previously unrecognized, but highly desirable properties of good producers of purine pathway‐related biochemicals have been identified for optimally engineered product biosynthesis; high capacity for reoxidation of NADPH and high bioenergetic efficiency. Reoxidation of NADPH, through the transhydrogenase or otherwise, appears to be particularly important for growth on glucose, as deduced from the corresponding optimal carbon flux distribution. The importance of cellular energetics on optimal performance was quantitatively assessed by including a bioenergetic efficiency parameter as an unrestricted, ATP dissipating flux in the simulations. An estimate for the bioenergetic efficiency was generated by fitting the model to experimentally determined growth yields. The results show that the maximum theoretical yields of all products studied are limited by pathway stoichiometry at high bioenergetic efficiencies. Simulations with the estimated bioenergetic efficiency of B. subtilis, growing under glucose‐limiting conditions, indicate that the yield of these biochemicals is primarily limited by energy and thus is very sensitive to the process conditions. The maximum yields that can reasonably be expected with B. subtilis on glucose were estimated to be 0.343, 0.160, and 0.161 (mol product/mol glucose) for purine nucleosides, riboflavin, and folic acid, respectively. Potential strategies for improving these maximum yields are discussed. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59: 227–238, 1998.

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