A polymerase chain reaction (PCR) assay was developed for discrimination of
Bacillus subtilis from other members of
B. subtilis group as well as rapid
identification from environmental samples. Primers ENIF and EN1R from endoglucanase
gene were used to amplify a1311 bp DNA fragment. The specificity of the primers was
tested with seven reference strains and 28 locally isolated strains of endoglucanase
positive Bacillus species. The PCR product was
only produced from B. subtilis. The results
demonstrated high specificity of two oligonucleotides for B.
subtilis. This species-specific PCR method provides a quick, simple,
powerful and reliable alternative to conventional methods in the detection and
identification of B. subtilis. To our knowledge
this is the first report of a B. subtilis
specific primer set.
Brevibacillus borstelensis cifa_chp40 is a thermophilic, strictly aerobic gram positive motile bacteria isolated from the alkaline hot water spring located in the Eastern Ghats zone of India. It could grow in a wide range of temperature and degrade low-density polythene at 37°C. The strain cifa_chp40 produces essential enzymes like protease, lipase, esterase and amidase at 50°C. Here, we report the draft genome sequence of B. borstelensis cifa_chp40 which will provide further insight into the metabolic capabilities, function and evolution of this important organism.
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