Fermentation monitoring

Raju, Gokaraju K.; Cooney, Charles L.

doi:10.1016/0958-1669(92)90123-z

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…Specialized books (Carr-Brion, 1991;BlakeColeman, 1993; Montague, 1997) and reviews (Montague et al, 1989;Raju and Cooney, 1992; automation is also included to emphasize the utility of real-time measurements for subsequent control purposes.…”

Section: Introductionmentioning

confidence: 99%

Monitoring of Submerged Bioprocesses

Vaidyanathan

Macaloney

Vaughan

et al. 1999

Critical Reviews in Biotechnology

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Monitoring is an important exercise in the operation of submerged bioprocesses and is a subject that has attracted intense research activity in recent years. In order to achieve optimal production or conversion, the factors that influence the performance of a bioprocess should be measured, preferably on-line, so that the process can be monitored in real-time and appropriate control or remedial action implemented. Such measurements can be achieved either in situ or ex situ. Ideally, in situ approaches are desirable. Where such an option is difficult to implement, ex situ approaches with appropriate sample handling techniques can be employed. In cases where direct analytical information is not available, inferential approaches can be adopted. In addition, analytical information not yet accessible on-line can be obtained off-line. Computational techniques have enabled the compilation of measurement data before utilizing the information for process diagnosis. Current developments in the different measurement technologies are presented with a critical assessment of their potential application in industrial production or process development.

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Section: Introductionmentioning

confidence: 99%

Monitoring of Submerged Bioprocesses

Vaidyanathan

Macaloney

Vaughan

et al. 1999

Critical Reviews in Biotechnology

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“…For the sucrose determination, invertase was co-immobilized with permeabilized cells within the membrane, and the production of hydrogen ion was detected using a pH electrode. From a practical standpoint, the fast and accurate determination of operating parameters such as substrate or product level is a prerequisite for the control and optimization of bioprocesses (Raju and Cooney, 1992). As a solution to this requirement, a flow injection analysis (FIA) system was developed, and its applications have been reported (Garn et al, 1989;Renneberg et al, 1991;Valero et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Flow Injection Analysis of Glucose, Fructose, and Sucrose Using a Biosensor Constructed with Permeabilized Zymomonas mobilis and Invertase

Park¹,

Shin²,

Lee³

et al. 1995

Biotechnology Progress

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Cells of Zymomonas mobilis possessing glucose-fructose oxidoreductase and gluconolactonase were permeabilized with toluene and co-immobilized with invertase within a gelatin membrane. This membrane was coated over a pH electrode, and the resulting biosensor was placed in a flow-through cell to develop a flow injection analysis (FIA) system for the specific determination of glucose, fructose, and sucrose. Peak height resulting from the production of hydrogen ion was correlated with sugar concentration, and the effect of operating variables on the response characteristics of the FLA system was investigated on the basis of theoretical and experimental analyses. Under the optimized conditions, the calibration curves for glucose, fructose, and sucrose were linear up to 8, 80, and 60 g/L, respectively. The FIA system was applied to the online monitoring of glucose production in the enzymatic hydrolysis of cellulose, and the glucose concentrations determined using the FIA system coincided well with those determined by the conventional enzymatic method. 863-869.

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