Fermentation characteristics and aerobic stability of grass silage inoculated with <i>Lactobacillus buchneri</i>, with or without homofermentative lactic acid bacteria

Driehuis, F.; Elferink, S.J.W.H. Oude; Wikselaar, P.G. van

doi:10.1046/j.1365-2494.2001.00282.x

Cited by 243 publications

(258 citation statements)

References 28 publications

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“…According to Driehuis et al (2001), the L. buchneri-treated corn silage is associated with a relative increase in the pH during the storage phase because of the high metabolic activity of L. buchneri in these silages. Although the final pH values were similar between silages in the present study, in the treated silages, the decrease in pH may have been less pronounced than in the untreated ones, which provided greater proteolysis, resulting in higher concentrations of ammonia in treated silages.…”

Section: Resultsmentioning

confidence: 99%

Fermentation and aerobic stability of corn silage inoculated with Lactobacillus buchneri

et al. 2012

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-The characteristics of fermentation and aerobic stability were evaluated in corn silage inoculated with different doses of Lactobacillus buchneri. The whole corn plant (300 g/kg DM) was ensiled in quadruplicate laboratory silos (7L). L. buchneri 40788 was applied at 5×10 4 , 1×10 5 , 5×10 5 and 1×10 6 cfu/g of fresh forage. Silages with no additive were used as controls. After 130 d of ensiling, the silages were subjected to an aerobic stability evaluation for 12 days, in which chemical and microbiological parameters as well as the temperature of the silage were measured to determine the aerobic deterioration. The addition of L. buchneri resulted in increased acetic acid concentrations. The number of yeast colonies was low in all treated silages. The pH, lactic and propionic acid concentrations did not differ between silages. Under aerobic conditions, all the treated silages showed a low number of yeasts and a great aerobic stability. Therefore, L. buchneri is effective against yeasts and improves the aerobic stability of corn silage in laboratory silos. However, doses equal or superior to 1×10 5 cfu/g of fresh forage were more efficient in the control of aerobic spoilage.

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Section: Resultsmentioning

confidence: 99%

Fermentation and aerobic stability of corn silage inoculated with Lactobacillus buchneri

et al. 2012

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“…According Driehuis et al (2000), the main objectives of using homofermentative bacteria in silage include reducing the risk of proliferation of bacteria of the genus Clostridium thereby reducing the production of butyric acid.…”

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confidence: 99%

Use of additives and pre-wilting in Tifton 85 bermudagrass silage production

et al. 2014

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The use of tropical grasses silage has become common in ruminant feed. An experiment was conducted to evaluate the fermentation characteristics, nutritional value, pH, fermentative capacity, ammonia nitrogen / total nitrogen (NH3N/total N) of Tifton 85 bermudagrass grass silage with different additives and wilting. The treatments were: pre-drying in the sun for two hours before silage, use of inoculant-enzymatic addition of soybean hulls, corn grits addition and use of salt in the surface layer of the silo. The experimental design was completely randomized with 6 treatments and 4 replications. Plants of Tifton 85 bermudagrass with 38 days of growth were ensiled in experimental silos with Bunsen valve type with packing densities of 236 kg of silage per m 3 for Tifton 85 bermudagrass pre-dried in the sun and 294 kg of silage per m 3 for the other treatments. The proportions of soybean hulls and corn grits added to the silage were calculated based on the initial DM content of Tifton 85 bermudagrass order to obtain MS 320 g kg -1 for the material to be ensiled. The buffering capacity did not differ between treatments in getting 29.56 (meqHCl/100gMS). It was found that the concentration of ammonia nitrogen did not differ between silages and remained low (3.22 g kg -1 ), the pH after silo opening was also similar with an average of 4.09 getting above 4.2 only in Tifton 85 bermudagrass silage without pre-treatment. The crude protein was higher in silages Tifton 85 bermudagrass and Tifton 85 bermudagrass with soybean hulls (17.48 g kg -1 ). The use corn grits caused a reduction in the values of NDF. The use of salt on the surface the layer reduced the production of latic and acetic acid.Index terms: Acid latic, corn grits, enzymatic-bacterian inoculant, ammonia nitrogen, soybean hulls. RESUMOO uso de silagem de gramíneas tropicas tem se tornado comum na alimentação de ruminante. Conduziu-se um experimento com o objetivo de avaliar o perfil fermentativo e valor nutricional da silagem de capim Tifton 85 com diferentes aditivos e emurchecimento. Os tratamentos foram: pré-secagem ao sol por 2 horas antes de ensilar, uso de inoculante bacteriano-enzimático, adição de casca de soja, adição de quirera de milho e uso de sal grosso na camada superficial do silo. O delineamento experimental foi inteiramente casualizado, com 6 tratamentos e 4 repetições. Plantas de capim Tifton 85 com 38 dias de crescimento foram ensiladas em silos experimentais com válvula tipo Bunsen com densidades de compactação de 236 kg de silagem por m 3 para o Tifton 85 pré-seco ao sol e 294 kg de silagem por m 3 para os demais tratamentos. As proporções de casca de soja e quirera de milho, adicionados à silagem, foram calculados com base no teor de MS inicial do Tifton 85 visando à obtenção de MS de 320 g kg -1 para o material a ser ensilado. A capacidade tampão não diferiu entre tratamentos ficando em 29,56 (meqHCl/100gMS). Verificou-se que os teores de nitrogênio amoniacal não diferiram entre as silagens e mantiveram-se baixos (32,2 g kg -1 ), o ...

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“…Driehuis et al (1999) and Nishino et al (2003) demonstrated the inhibitory effects of acetic acid on fungi. The results achieved by Ranjit and Kung (2000), Driehuis et al (2001) and Filya (2003a) showed that inoculation with L. buchneri alone or in combination with homofermentative LAB impairs the growth of moulds on the surface of silage. Acetic acid acts as a growth inhibitor of spoilage organisms by decreasing the maximum growth rate and, therefore, acetic acid increases the aerobic stability (Holzer et al 2003).…”

Section: Discussionmentioning

confidence: 97%

Ensiling corn silage with different levels of a multi-species lactic acid bacteria inoculant

2014

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Abstract.A multi-species lactic acid bacterial inoculant (Lactisil maize, LM) was applied to whole-crop corn at different maturities in laboratory silos, to evaluate its effects on biochemical characteristics and aerobic stability. The corn crop was harvested at hard dough (HD, 253.1 g/DM kg), one-third milkline (ML, 293.7 g/DM kg) and one-third milkline with a killing frost (MLF, 297.6 g/DM kg). Crops were chopped to a 2.5-cm theoretical cut length, subsampled and treated with two levels of inoculant (LB1 = 1.5 · 10 5 cfu/g forage, LB2 = 3 · 10 5 cfu/g forage) or untreated (WO). The chemical composition of MLF crops was very similar to that of ML crops. However, lower (P < 0.01) numbers of lactic acid bacteria and higher numbers of yeast were enumerated in MLF than in ML crops. Higher percentages of DM and neutral detergent fibre and higher pH, but lower (P < 0.01) concentrations of water soluble carbohydrate and crude protein were measured in ML and MLF crops than in HD crops. Application of the inoculant increased (P < 0.01) concentrations of volatile fatty acids, neutral detergent fibre and acid detergent fibre in silages. Lactic acid concentration increased (P < 0.01) in HD treatments with an increasing level of inoculant. In contrast, the highest (P < 0.01) lactic acid concentration was measured in LB1 treatment compared with WO and LB2 in ML and MLF silages. Silages prepared from ML and MLF crops had higher (P < 0.01) lactic and acetic acid concentrations but lower (P < 0.01) butyric acid concentrations than did those prepared from HD. The pH in LB1 and LB2 silages was higher (P < 0.01) than that measured in WO silages. Aerobic stability was not influenced by inoculant treatment but low-DM silages were more (P < 0.01) resistant to spoilage. Frost-killed corn crops had a good potential to produce well fermented silage. Using LM resulted in silages with slightly higher fermentation products but it failed to improve aerobic stability of silage after 120 days of ensiling. These results indicated that inoculation of corn crops with LM for a short-duration ensilage period cannot enhance aerobic stability of silages due to insufficient acetic acid production from lactic acid conversion.Additional keywords: aerobic stability, frost-killed corn, heterofermentative fermentation, homofermentative lactic acid bacteria, Lactobacillus buchneri, maturity stage.

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Fermentation characteristics and aerobic stability of grass silage inoculated with Lactobacillus buchneri, with or without homofermentative lactic acid bacteria

Cited by 243 publications

References 28 publications

Fermentation and aerobic stability of corn silage inoculated with Lactobacillus buchneri

Fermentation and aerobic stability of corn silage inoculated with Lactobacillus buchneri

Use of additives and pre-wilting in Tifton 85 bermudagrass silage production

Ensiling corn silage with different levels of a multi-species lactic acid bacteria inoculant

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