FERMENTAÇÃO ALCOÓLICA DE HIDROLISADO HEMICELULÓSICO DE TORTA DE GIRASSOL POR Galactomyces geotrichum UFVJM-R10 E Candida akabanensis UFVJM-R131

Abstract

ALCOHOLIC FERMENTATION OF HEMICELLULOSIC HYDROLYZATE FROM SUNFLOWER CAKE BYGalactomyces geotrichum UFVJM-R10 AND Candida akabanensis UFVJM-R131. The use of the hemicellulosic fraction of plants for the production of second generation bioethanol depends on microorganisms capable to ferment pentoses. Two yeast strains habile to xylose fermenting in synthetic medium, Candida akabanensis UFVJM-R131 and Galactomyces geotrichum UFVJM-R10, not yet registered in the literature for the production of bioethanol, were evaluated here in the alcoholic fermentation of the hemicellulosic hydrolyzate from sunflower cake. The biomass hydrolysis was performed by 38 minutes at 120 °C / 1 atm with 6% H 2 SO 4 solution and solid / liquid ratio of 1:3.2. Chromatographic characterization of the hemicellulosic hydrolyzate showed the presence of glucose (7.57 g L ). Both yeasts were able to produce ethanol from the acid hydrolyzate from sunflower cake. The fermentation carried out with G. geotrichum UFVJM-R10 and C. akabanensis UFVJM-R131 presented Y P/S values of 0.29 and 0.27 g ethanol g -1 sugars , respectively. The amounts of the inhibitors identified in the hydrolyzate did not affect the efficiency of the alcoholic fermentation. The supplementation of the hydrolyzate with nitrogen and mineral sources increased the rate of consumption of xylose and arabinose.

Fermentation profiles of the yeast Brettanomyces bruxellensis in d‐xylose and l‐arabinose aiming its application as a second‐generation ethanol producer

“…The preference for d ‐xylose over l ‐arabinose has already been described in a modified strain of S. cerevisiae (Wisselink, Toirkens, Wu, Pronk, & van Maris, 2009). In contrast, Candida akabanensis UFVJM‐R131 is capable of using both sugars simultaneously, converting them to ethanol (de Matos, Souza, Santos, & Pantoja, 2018). Interestingly, in aerobiosis, ethanol yield and productivity values in the mixed medium were higher than those observed in glucose‐based medium (Tables 3 and 4).…”

Xylose fermentation to ethanol by newGalactomyces geotrichumandCandida akabanensisstrains

“…In a recently published work by our research group ( Matos et al, 2018 ), the microbial isolates described herein were able to ferment the hemicellulosic hydrolyzate obtained by acid treatment of the sunflower seed cake. In that work, isolates UFVJM-R10 and UFVJM-R131 presented Y P∕S values of 0.29 and 0.27 g ethanol g −1 sugars, respectively, and were capable to cofermenting xylose and glucose.…”

“…At the end of each fermentation, the concentration of ethanol was determined using a spectrophotometric method ( Isarankura-Na-Ayudhya et al, 2007 ). The production of ethanol was confirmed by HPLC, as described by Matos et al (2018) . The product yield by substrate (Y P∕S , g g −1 ) was calculated as the ratio between the amount of ethanol produced and the amount of reducing sugars consumed at end of fermentative process; the ethanol yield by cell growth (Y P∕X , g g −1 ) was calculated as the ratio between the amount of ethanol produced and the amount of cell biomass formed at end of fermentative process; the fermentation efficiency (Ef%) were calculated as ratio between Y P∕S and theoretical alcoholic fermentative yield (0.511); the volumetric productivity (Q P , g L −1 h −1 ), which is the ratio between the final concentration of ethanol and the fermentation time, also was acessed.…”

Estudo Da Atividade Fotoprotetora De Extratos De Caule, Flor, Raiz E Frutos De Cacto Xique-Xique (Pilosocereus Gounellei)

“…Nos anos 2000, novas linhagens naturalmente capazes de fermentar pentoses foram descritas, tais como Spathaspora passalidarum (NGUYEN et al, 2006;HOU, 2012) e Spathaspora arboriae (CADETE et al, 2009;CUNHA-PEREIRA et al, 2011) (RYABOVA et al, 2003;DMYTRUK et al, 2008;GALAFASSI et al, 2011;NITIYON et al, 2011;RODRUSSAMEE et al, 2011;VERHO et al, 2011;CADETE et al, 2013;DOGARIS et al, 2013;LORLIAM et al, 2013;KALHORINIA et al, 2014;SUKPIPAT et al, 2017;MATOS et al, 2017;VALINHAS et al, 2018). Nesta mesma década, Barbosa (2017) fez o primeiro relato na literatura da espécie Sporothrix schenckii, anteriormente mencionada apenas como causadora de doença, como sendo capaz de converter xilose em bioetanol.…”