FePt nanoparticles as a potential X-ray activated chemotherapy agent for HeLa cells

Abstract

Nanomaterials have an advantage in “personalized” therapy, which is the ultimate goal of tumor treatment. In order to investigate the potential ability of FePt nanoparticles (NPs) in the diagnosis and chemoradiotherapy treatment of malignant tumors, superparamagnetic, monodispersed FePt (~3 nm) alloy NPs were synthesized, using cysteamine as a capping agent. The NPs were characterized by means of X-ray diffraction; transmission electron microscopy, Physical Property Measurement System, and Fourier transform infrared spectroscopy. The cytotoxicity of FePt NPs on Vero cells was assessed using an MTT assay, and tumor cell proliferation inhibited by individual FePt NPs and FePt NPs combined with X-ray beams were also collected using MTT assays; HeLa human cancer cell lines were used as in vitro models. Further confirmation of the combined effect of FePt NPs and X-rays was verified using HeLa cells, after which, the cellular uptake of FePt NPs was captured by transmission electron microscopy. The results indicated that the growth of HeLa cells was significantly inhibited by FePt NPs in a concentration-dependent manner, and the growth was significantly more inhibited by FePt NPs combined with a series of X-ray beam doses; the individual NPs did not display any remarkable cytotoxicity on Vero cells at a concentration <250 μg/mL. Meanwhile, the FePt NPs showed negative/positive contrast enhancement for MRI/CT molecule imaging at the end of the study. Therefore, the combined results implied that FePt NPs might potentially serve as a promising nanoprobe for the integration of tumor diagnosis and chemoradiotherapy.

Overcoming radiation resistance by iron-platinum metal alloy nanoparticles in human copper transport 1-overexpressing cancer cells via mitochondrial targeting

“…Investigations of the potential applications in the diagnostic and therapeutic elds of cancer have been carried out due to their excellent physicochemical characteristics. Several studies were conducted investigating FePt NPs as dual MRI/CT imaging contrast [20,26], drug delivery system [27], photothermic or hyperthermia agent [28], and chemo-radiation sensitizers [29,30]. Our ndings that FePt NPs can overcome radioresistance further support their roles in radiotherapy of cancer treatments.…”

A Comprehensive Review on the Synthesis, Characterization, and Biomedical Application of Platinum Nanoparticles

“…Bimetallic NPs such as iron platinum (Fe-Pt) NPs possess unique chemical and magnetic properties such as chemical stability, superparamagnetization, high Curie temperature, high saturation magnetization, and high X-ray absorption. These unique properties provide the potential of their application in hyperthermia treatment, as MRI contrast agents, in drug delivery, and as biosensors [142][143][144][145][146][147][148][149][150][151][152][153][154][155][156][157][158][159].…”

Nanoparticles in biomedical applications

“…Bimetallic nanoparticles such as iron platinum (Fe-Pt) possess unique chemical and magnetic properties such as chemical stability, superparamagnetisation, high Curie temperature, high saturation magnetisation and high X-ray absorption [155][156][157][158]. These properties make them attractive materials for use in hyperthermia treatment [159,160], MRI contrast agents [161][162][163][164][165][166], drug delivery [167][168][169][170][171] and biosensors [172] which will be discussed in more detail in Section 4.…”

“…This review investigates other nanosystems such as metallic nanoparticles like gold (Au) [112][113][114][115][116][117][118][119][120][121][122][123][124][125][126][127][128][129][130] and silver (Ag) , bimetallic nanoparticles like iron cobalt (Fe-Co) [78][79][80][92][93][94][95][96][97]108,109] and iron platinum (Fe-Pt) [155][156][157][158][159][160][161][162][163][164][165][166][167][168][169][170][171][172] and metal oxides including titanium dioxide (TiO 2 ) [1...…”

“…It is due to these properties that research for the potential uses of Fe-Pt nanoparticles in biomedical applications such hyperthermia [159,160] as contrast agents for MRI [161][162][163][164][165][166], drug delivery/cancer therapy [167][168][169][170][171] and biosensors [172] has increased. In this review, we will discuss the use of Fe-Pt nanoparticles in targeted drug delivery, cancer therapy, MRI contrast agents as well as its possible use in magnetic hyperthermia.…”