Fenton chemistry in biology and medicine

Prousek, Josef

doi:10.1351/pac200779122325

Cited by 373 publications

(279 citation statements)

References 114 publications

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“…A higher accumulation of H 2 O 2 under stress signifies sub-optimal functioning of H 2 O 2 metabolizing enzymes. Stress induced H 2 O 2 can also react with superoxide radicals to form highly reactive hydroxyl radicals (Prousek 2007), in metal catalyzed fenton reaction. These hydroxyl radicals initiate self-propagating reactions leading to peroxidation of membrane lipids and destruction of proteins, finally leading to cell death (Jaw and Ching 1998).…”

Section: Resultsmentioning

confidence: 99%

Responses to drought induced oxidative stress in five finger millet varieties differing in their geographical distribution

Bhatt

Negi

Sharma

et al. 2011

Physiol Mol Biol Plants

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The study presents the impact of drought stress on five finger millet varieties (PR202, VL146, VL315, PES400 and VR708), representing contrasting areas of Indian sub-continent. Drought stress induced increase in the activity of superoxide dismutase, ascorbate peroxidase and glutathione reductase was higher in PR202 and VL315, while the activity was lower in the varieties PES400 and VR708. Ascorbate peroxidase : superoxide dismutase ratio, which is a crucial factor in alleviating drought stress, was higher in varieties PR202 and VL315, whilst the varieties PES400 and VR708 exhibited a lower ratio under stress. The variety PES400 recorded maximum stress induced damage, as indicated by higher accumulation of malondialdehyde and hydrogen peroxide; whereas the variety PR202 recorded least stress induced cytotoxic damage. The results clearly indicate that better drought tolerance of the variety PR202 is positively related to the capacity of its antioxidant system to scavenge reactive oxygen species, resulting in a reduced incidence of oxidative damage. Ascorbate peroxidase : superoxide dismutase ratio is found to be a critical factor governing the stress tolerance potential of different varieties. Therefore, varieties PR202 and VL315 were found to be tolerant while PES400 was susceptible to drought stress.

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Section: Resultsmentioning

confidence: 99%

Responses to drought induced oxidative stress in five finger millet varieties differing in their geographical distribution

Bhatt

Negi

Sharma

et al. 2011

Physiol Mol Biol Plants

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“…The lgt mutation could have an effect on the levels of many transporters (or other important proteins) and their activity. Factors such as divalent cations (Prousek, 2007) are known to be able to enhance the effect of oxidative stress. The phenotype observed here could (at least in part) be related to a reduction of the ability of the Slgt mutant to transport these compounds.…”

Section: Phenotypic Characterization Of the Slgt Mutantmentioning

confidence: 99%

The prolipoprotein diacylglyceryl transferase (Lgt) of Enterococcus faecalis contributes to virulence

et al. 2012

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Enterococcus faecalis is an opportunistic pathogen responsible for nosocomial infections. Lipoproteins in Gram-positive bacteria are translocated across the plasma membrane and anchored by the fatty acid group. They perform critical roles, with some described as virulence determinants. The aim of this study was to explore the roles of E. faecalis lipoproteins in the stress response and virulence. We constructed a mutant affected in the predicted prolipoprotein diacylglyceryl transferase gene lgt, and examined the role of Lgt in membrane anchoring, growth, the stress response and virulence. Inactivation of lgt enhanced growth in a high concentration of Mn2+ or under oxidative stress in vitro, and significantly decreased virulence

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“…Similar to unsaturated lipids, bsitosterol can undergo autoxidation by various oxygen species, including reactive oxygen species, hydrogen peroxide, and hydroxyl radical (OHc). 39 During MALDI irradiation, the free radical OHc could be produced by the decomposition of DHB + c. 40 The highly reactive radical OHc (diffusion-controlled, i.e., k z 10 9 M À1 s À1 ) 41 frequently attacks lipid molecules by abstracting hydrogen, predominantly at C-7 for sterols, usually setting off free-radical chain reactions. 42 Consequently, OHc could react with molecules forming the fatty acid, alkoxy and peroxy radicals 43 and nally forming primary products (hydroperoxides), which decompose into secondary oxidation products with different chemical functional groups, such as hydroxy, epoxy, and keto groups.…”

Section: Matrix Compoundsmentioning

confidence: 99%

Analysis of phytosterol by MALDI-TOF mass spectrometry

Liang

Shu

et al. 2014

Anal. Methods

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-Sitosterol is the most abundant phytosterol lipid in plants. This study demonstrates a method for analyzing b-sitosterol using a laboratory-built MALDI-TOF mass spectrometer. The matrix compound, solvent system, laser intensity, alkali ion, and analyte concentration for sample preparation were optimized to acquire reproducible and informative mass spectra of the analyte. The best MALDI spectrum of b- 12.1 and 1207.5 mM. The detection limit was 10 nM. These results demonstrate the potential application of MALDI mass spectrometry for analyzing phytosterols.

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Fenton chemistry in biology and medicine

Cited by 373 publications

References 114 publications

Responses to drought induced oxidative stress in five finger millet varieties differing in their geographical distribution

Responses to drought induced oxidative stress in five finger millet varieties differing in their geographical distribution

The prolipoprotein diacylglyceryl transferase (Lgt) of Enterococcus faecalis contributes to virulence

Analysis of phytosterol by MALDI-TOF mass spectrometry

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