Femtosecond laser modification of living neuronal network

Hosokawa, Chie; Kudoh, Suguru N.; Kiyohara, Ai; Hosokawa, Yoichiroh; Okano, Kazunori; Masuhara, Hiroshi; Taguchi, Takahisa

doi:10.1007/s00339-008-4655-3

Cited by 25 publications

(23 citation statements)

References 15 publications

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“…Advantages of ultrafast laser micromachining of materials have been established over the last decades and numerous potential applications have been tested, particularly for thin film deposition, MEMs fabrication, surface micromachining, 3D internal waveguide fabrication, nanotechnology and surgical applications [1][2][3][4][5][6]. Because of the very short temporal and spatial scales and extremely high intensity, complicated optical, thermodynamic, chemical and mechanical processes are thought to be involved during ultrafast laser micromachining [7].…”

Section: Introductionmentioning

confidence: 99%

Effects of laser operating parameters on metals micromachining with ultrafast lasers

Cheng

Perrie

Edwardson

et al. 2009

Applied Surface Science

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Section: Introductionmentioning

confidence: 99%

Effects of laser operating parameters on metals micromachining with ultrafast lasers

Cheng

Perrie

Edwardson

et al. 2009

Applied Surface Science

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“…The mechanism of neuronal disruption by a focused femtosecond laser is discussed in more detail elsewhere [15]. Briefly, when a femtosecond laser is focused on neurons, multiphoton absorption of femtosecond pulses occurs only at the focal point.…”

Section: Discussionmentioning

confidence: 99%

“…Simultaneously, the cut neurite shrunk owing to the relaxation of intracellular tension and disruption of the adhesion between the cell and the substrate [15]. When the femtosecond laser was scanned along one line of electrodes in a MEA dish, the neurons along the scanning line were removed (Fig.…”

Section: Scission Of Neurons By Femtosecond Lasermentioning

confidence: 99%

Resynchronization in neuronal network divided by femtosecond laser processing

Hosokawa¹,

Kudoh

Kiyohara³

et al. 2008

NeuroReport

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We demonstrated scission of a living neuronal network on multielectrode arrays (MEAs) using a focused femtosecond laser and evaluated the resynchronization of spontaneous electrical activity within the network. By an irradiation of femtosecond laser into hippocampal neurons cultured on a multielectrode array dish, neurites were cut at the focal point. After the irradiation, synchronization of neuronal activity within the network drastically decreased over the divided area, indicating diminished functional connections between neurons. Cross-correlation analysis revealed that spontaneous activity between the divided areas gradually resynchronized within 10 days. These findings indicate that hippocampal neurons have the potential to regenerate functional connections and to reconstruct a network by self-assembly.

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“…Ideally, nanoprobes, therefore, need to be designed with high resolution both spatially and temporally, and also to have a precise selectivity for the biological molecule of interest. In previous studies, therefore, nanoprobing and manipulation of the biological materials were done using a silicon nano needle[3] and high speed scanning [ 4] by an atomic force microscope, a magnetic bead with a labeling of an antibody,[5] a scanning femtosecond laser for cell ablation, [6] 100-nm thick SiN nanomembrane placed between the electron-beam and fluorescence microscope to separate an atmospheric environment and a vacuum environment. The nanomembrane was also worked as a cell culture surface and an energy transfer window of electron beam energy.…”

Section: Introductionmentioning

confidence: 99%

Closed-looped nano stimulation microscope for living cell membrane

Hoshino

Morishima

2012

2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)

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Electron-beam could stimulate a living cell membrane through a tOO-nm-thick SiN nanomembrane. We designed a co-axial dual microscope with an electron-beam lithography system and a fluorescence microscope to investigate bio-molecular dynamic system of living cell membrane. This microscope had a closed-looped controlling system using the fluorescence live cell imaging and the electron beam induced stimulation. The live cell imaging of the fluorescence microscope provides the chemical identity of the target molecule and the response to a following stimulation on the cell membrane, and the electron-beam can induce electro-chemical stimulation in hundreds nanometer resolution. Scanning of the electron-beam could provide high-speed, precise, and large scale stimulation on the target molecules. We attempted that the combination with this stimulation system and fluorescence microscope would provide the closed-loop control of the bio-molecule system on cell membrane. We proposed here the concept of the virtual molecule environmental display to study the functional changes on the target behavior due to our closed-loop control system on this co axial microscope.

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Femtosecond laser modification of living neuronal network

Cited by 25 publications

References 15 publications

Effects of laser operating parameters on metals micromachining with ultrafast lasers

Effects of laser operating parameters on metals micromachining with ultrafast lasers

Resynchronization in neuronal network divided by femtosecond laser processing

Closed-looped nano stimulation microscope for living cell membrane

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