Femtosecond laser microstructured Alumina toughened Zirconia: A new strategy to improve osteogenic differentiation of hMSCs

Carvalho, Ângela; Cangueiro, Liliana; Oliveira, V.; Vilar, R.; Fernandes, Maria Helena; Monteiro, Fernando J.

doi:10.1016/j.apsusc.2017.11.206

Cited by 52 publications

(26 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Still, the evaluation of the osteogenic commitment of hMSCs over long-term cell cultures has yet to be reported in the literature. Other preliminary data on ditch-like structures obtained by fs laser ablation on pre-sintered polished Alumina-Toughened Zirconia ATZ disks, using a Yb:KYW chirped-pulse-regenerative amplification laser system (1030 nm, 500 fs) show the alignment of hMSCs on the developed microgroove surfaces (width < 20 µm with the depth of approximately 2.6 µm), as well as more efficient osteogenic differentiation onto the micro-grooved surfaces [ 43 , 44 ].…”

Section: Introductionmentioning

confidence: 99%

Bioinstructive Micro-Nanotextured Zirconia Ceramic Interfaces for Guiding and Stimulating an Osteogenic Response In Vitro

Sima

Bonciu

Baciu³

et al. 2020

Nanomaterials

View full text Add to dashboard Cite

Osseous implantology’s material requirements include a lack of potential for inducing allergic disorders and providing both functional and esthetic features for the patient’s benefit. Despite being bioinert, Zirconia ceramics have become a candidate of interest to be used as an alternative to titanium dental and cochlear bone-anchored hearing aid (BAHA) implants, implying the need for endowing the surface with biologically instructive properties by changing basic parameters such as surface texture. Within this context, we propose anisotropic and isotropic patterns (linear microgroove arrays, and superimposed crossline microgroove arrays, respectively) textured in zirconia substrates, as bioinstructive interfaces to guide the cytoskeletal organization of human mesenchymal stem cells (hMSCs). The designed textured micro-nano interfaces with either steep ridges and microgratings or curved edges, and nanoroughened walls obtained by direct femtosecond laser texturing are used to evaluate the hMSC response parameters and osteogenic differentiation to each topography. Our results show parallel micro line anisotropic surfaces are able to guide cell growth only for the steep surfaces, while the curved ones reduce the initial response and show the lowest osteogenic response. An improved osteogenic phenotype of hMSCs is obtained when grown onto isotropic grid/pillar-like patterns, showing an improved cell coverage and Ca/P ratio, with direct implications for BAHA prosthetic development, or other future applications in regenerating bone defects.

show abstract

Section: Introductionmentioning

confidence: 99%

Bioinstructive Micro-Nanotextured Zirconia Ceramic Interfaces for Guiding and Stimulating an Osteogenic Response In Vitro

Sima

Bonciu

Baciu³

et al. 2020

Nanomaterials

View full text Add to dashboard Cite

show abstract

“…Previously, we reported the use of a femtosecond laser technique on the surface of ATZ, developing microgrooves with overlapping highfrequency LIPSS (laser induced periodic surface structures) that modulated cells adhesion, proliferation and induced increased osteogenic differentiation when compared with the non-treated control [25].…”

Section: Discussionmentioning

confidence: 99%

“…Femtosecond laser machining has already been proposed as an innovative technique to create topographical features on the surface of ceramics, commonly used in the fields of optical and refractory materials. Some studies have reported the use of femtosecond laser to texture the surface of hard ceramics [19][20][21], although little work has been conducted on periodic surface structuring, particularly with the intent of implants development, with scarce results available on the biological response to these materials [22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Femtosecond laser microstructuring of alumina toughened zirconia for surface functionalization of dental implants

Carvalho

Grenho

Fernandes

et al. 2020

Ceramics International

Self Cite

View full text Add to dashboard Cite

The continuous need for high-performance implants that can withstand mechanical loads while promoting implant integration into bone has focused recent research on the surface modification of hard ceramics. Their properties of biocompatibility, high mechanical and fatigue resistance and aesthetic color have contributed to its succefull applications in dentistry. Alumina toughened Zirconia (ATZ) has been gaining attention as a material for dental implants applications due to its advanced mechanical properties and minimal degradation at body temperature. Still, in order to improve tissue response to this bioinert material, additional modifications are desirable. Improving the surface functionality of this ceramic could lead to enhanced implant-tissue interaction and subsequently, a successful implant integration. In this work, microtopographies were developed on the surface of Alumina toughened Zirconia using an ultrafast laser methodology, aiming at improving the cellular response to this ceramic. Microscale grooves and grid-like geometries were produced on ATZ ceramics by femtosecond laser ablation, with a pulse width of 150 fs, wavelength of 800 nm and repetition rate of 1 kHz. The variation of surface topography, roughness, chemistry and wettability with different laser processing parameters was examined. Cell-surface interactions were evaluated for 7 days on both microstructured surfaces and a non-treated control with pre-osteoblasts, MC3T3-E1 cells. Both surface topographies showed to improve cell response, with increased metabolic activity when compared to the untreated control and modulating cell morphology up to 7 days. The obtained results suggest that femtosecond laser texturing may be a suitable non-contact methodology for creating tunable micro-scale surface topography on ATZ ceramics to enhance the biological response.

show abstract

“…Femtosecond laser was used to machine dental ceramic implants made of zirconia-toughened alumina (ZTA) [18], or to induce regular patterns on an alumina-toughened zirconia (ATZ) surface [19]. And similarly to what was attempted in monolithic zirconia, laser-modifications of alumina-zirconia composites were used to induce osteogenic differentiation of human mesenchymal stem cells [20] and favour viability of osteoblast-like cells [21], or to help osseointegration of endosseous dental implants [22]. In addition to the microstructural changes induced in zirconia by the melting/ablation/quenching sequence, alumina-zirconia composites show also chemical changes, as reported by Ackerl et al [18] who reported that "Alumina […] seemed to dissolve approaching the surface".…”

Section: Introductionmentioning

confidence: 99%

Microstructure and hydrothermal ageing of alumina-zirconia composites modified by laser engraving

Grémillard

Cárdenas

Reverón

et al. 2020

Journal of the European Ceramic Society

View full text Add to dashboard Cite

Laser engraving is more and more commonly used to write permanent identity features on medical devices. In particular, it is performed on ceramic heads for hip prostheses. Since these components are submitted to high mechanical loading during long periods of time (several years), it is critical to assess the influence of laser engraving on their durability. In the present article, laser marking of zirconia-toughened alumina and alumina-toughened zirconia resulted in an important colour change. It did not affect notably the resistance to hydrothermal ageing, despite important microstructural changes (in particular formation of a zirconia-alumina solid solution layer some tenths of micrometres thick). The formation of this original microstructure was explained by the fast cooling rate after lasing.

show abstract

Femtosecond laser microstructured Alumina toughened Zirconia: A new strategy to improve osteogenic differentiation of hMSCs

Cited by 52 publications

References 53 publications

Bioinstructive Micro-Nanotextured Zirconia Ceramic Interfaces for Guiding and Stimulating an Osteogenic Response In Vitro

Bioinstructive Micro-Nanotextured Zirconia Ceramic Interfaces for Guiding and Stimulating an Osteogenic Response In Vitro

Femtosecond laser microstructuring of alumina toughened zirconia for surface functionalization of dental implants

Microstructure and hydrothermal ageing of alumina-zirconia composites modified by laser engraving

Contact Info

Product

Resources

About