Femtosecond laser direct fabrication of micro-grooved textures on a capillary flow immunoassay microchip for spatially-selected antibody immobilization

Goya, Kenji; Yamachoshi, Yuji; Fuchiwaki, Masaki; Tanaka, Masato; Ooie, Toshihiko; Abe, Kaori; Kataoka, Masatoshi

doi:10.1016/j.snb.2016.09.056

Cited by 10 publications

(7 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Within our experimental parameters, the first structures started to emerge from 5 × 10 3 pulses ( Figure 2 b). These structures are probably formed around microscopic defects generation (hot points) and film roughness, as previously suggested [ 33 ]. These few-micrometers hot points grow and merge into larger areas.…”

Section: Resultsmentioning

confidence: 54%

“…Some polymers were structured in the bulk state by femtosecond laser, namely poly(methyl methacrylate) PMMA [ 33 ], polystyrene PS [ 34 ], polydimethylsiloxane PDMS [ 35 ], polytetrafluoroethylene PTFE [ 36 ], and polyether ether ketone PEEK [ 37 ]. Potential applications of structured polymer surfaces are nano-templating, storage media applications [ 3 ], and some specific solutions for biosystems [ 38 ].…”

Section: Introductionmentioning

confidence: 99%

“…By direct irradiation of PTFE plate [ 36 ], micro–nano dual-scale composite structures were created. The micro-grooves morphology of LIPSS was formed thanks to Ti:Sapphire laser on PMMA microchip [ 33 ]. Due to the particular properties of the polymers, such as low glass transition temperature (T g ), the LIPSS formations on polymer foils are often achieved with low beam fluencies (with a fluence of 10.5 and 12.5 mJ/cm 2 for PET and PS irradiated by nanosecond laser beam at 248 nm) [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Polystyrene Thin Films Nanostructuring by UV Femtosecond Laser Beam: From One Spot to Large Surface

et al. 2021

View full text Add to dashboard Cite

In this work, direct irradiation by a Ti:Sapphire (100 fs) femtosecond laser beam at third harmonic (266 nm), with a moderate repetition rate (50 and 1000 Hz), was used to create regular periodic nanostructures upon polystyrene (PS) thin films. Typical Low Spatial Frequency LIPSSs (LSFLs) were obtained for 50 Hz, as well as for 1 kHz, in cases of one spot zone, and also using a line scanning irradiation. Laser beam fluence, repetition rate, number of pulses (or irradiation time), and scan velocity were optimized to lead to the formation of various periodic nanostructures. It was found that the surface morphology of PS strongly depends on the accumulation of a high number of pulses (103 to 107 pulses) at low energy (1 to 20 µJ/pulse). Additionally, heating the substrate from room temperature up to 97 °C during the laser irradiation modified the ripples’ morphology, particularly their amplitude enhancement from 12 nm (RT) to 20 nm. Scanning electron microscopy and atomic force microscopy were used to image the morphological features of the surface structures. Laser-beam scanning at a chosen speed allowed for the generation of well-resolved ripples on the polymer film and homogeneity over a large area.

show abstract

Section: Resultsmentioning

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Polystyrene Thin Films Nanostructuring by UV Femtosecond Laser Beam: From One Spot to Large Surface

et al. 2021

View full text Add to dashboard Cite

show abstract

“…We have recently demonstrated antibody immobilization on a hemiwicking 17 polymer surface prepared using femtosecond (FS) laser microstructuring. 18 FS laser microstructuring enables the creation of superhydrophilic microgrooved structures that provide paper-like features such as liquid migration and absorptivity driven by capillary force. Using such hydrophilic structures, an antibody can selectively and precisely be immobilized in a spatially localized area.…”

Section: Introductionmentioning

confidence: 99%

“…Further details regarding the fabrication conditions are available in our previous work. 18 Figure 2 depicts the contact angle of a water droplet with a volume of 0.5 μL on a raw PMMA (a, b) surface and on a superhydrophilic grooved structure (c, d) fabricated on a PMMA substrate. The contact angle of the water on the raw PMMA is 70.4 .…”

Section: Introductionmentioning

confidence: 99%

Paper-like Surface Microstructure Fabricated on a Polymer Surface by Femtosecond Laser Machining

Goya

Fuchiwaki

2018

ANAL. SCI.

Self Cite

View full text Add to dashboard Cite

Experimental Laser fabrication systemFigures 1(a) and 1(b) show schematics of the experimental setup and method, respectively, used to fabricate microgrooves 2018 © The Japan Society for Analytical Chemistry † To whom correspondence should be addressed. E-mail: gouya-k@ile.osaka-u.ac.jp K. G. present address: Institute of Laser Engineering, Osaka University, Room 330, Bldg. I, 2-6 Yamadaoka, Suita, Osaka 565-0871, Japan. Paper-like Surface Microstructure Fabricated on a Polymer Surface by Femtosecond Laser MachiningKenji GOYA † and Yusuke FUCHIWAKI Health Research Institute, National Institute of Advanced Industrial Science and Technology, 2217-14 Hayashi, Takamatsu, Kagawa 761-0395, Japan In this study, we demonstrate the precise control of fluid flow using femtosecond (FS) laser-induced microstructures. A microgroove structure inscribed on a poly(methyl methacrylate) (PMMA) substrate functions as a superhydrophilic membrane similar to paper. We first estimated the flow rate for pure water on microgrooves fabricated at various laser fluences in the range from 9.2 to 100.8 J/cm 2 . The results showed that the flow rate could be tuned in the range from 0.30 to 12.07 μL/s by varying the laser irradiation parameters. The fluid flow was reproducible, with a calculated relative standard deviation (RSD%) of less than 8% in the flow rate. We then fabricated a microfilter for blood separation and estimated its filtration ability using artificial blood containing resin microparticles. This method would be useful in a technology related to a paper-based diagnostic device for precise reagent manipulation.

show abstract

Influence of Femtosecond Laser Parameters and Environment on Surface Texture Characteristics of Metals and Non-Metals – State of the Art

Bharatish

Santhanakrishnan

2018

Lasers Manuf. Mater. Process.

View full text Add to dashboard Cite

Femtosecond laser direct fabrication of micro-grooved textures on a capillary flow immunoassay microchip for spatially-selected antibody immobilization

Cited by 10 publications

References 39 publications

Polystyrene Thin Films Nanostructuring by UV Femtosecond Laser Beam: From One Spot to Large Surface

Polystyrene Thin Films Nanostructuring by UV Femtosecond Laser Beam: From One Spot to Large Surface

Paper-like Surface Microstructure Fabricated on a Polymer Surface by Femtosecond Laser Machining

Influence of Femtosecond Laser Parameters and Environment on Surface Texture Characteristics of Metals and Non-Metals – State of the Art

Contact Info

Product

Resources

About