Female Reproductive Performance in the Mouse: Effect of Oral Melatonin

Zhao, Xiaozhen; Wang, Dian; Wu, Zhenzheng; Pan, Bo; Yang, Huai; Zeng, Changjun; Zhang, Ming; Liu, Guoshi; Han, Hongbing; Zhou, Guangbin

doi:10.3390/molecules23081845

Cited by 12 publications

(8 citation statements)

References 40 publications

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“…Vitamins B are water-soluble compounds with antioxidant properties [ 109 ]. The role of B-vitamins in the field of fertility has been the subject of in vitro and in vivo studies [ 54 , 55 ]. The effects of vitamin B 9 (folic acid) were tested in vitro by exposing mouse oocytes treated with hypoxantine (inhibitor of mouse oocyte maturation) to 500 µM of folic acid.…”

Section: Agents Consideredmentioning

confidence: 99%

“…Severe B 1 deficiency diet was linked to alterations in the meiotic maturation of oocytes in a mouse model [ 55 ]. While the frequency of abnormal oocytes in ICR mice fed with a vitamin B 1 -free diet did not differ from that of the control groups after 13 days of diet, prolonging the vitamin B 1 -free diet up to 20 days lead to an increase of abnormal oocytes in the test mice than in the control group.…”

Section: Agents Consideredmentioning

confidence: 99%

“…Aberrant eggs presented spindle defects and growth arrest at the germinal vesicle (GV) stage. After feeding mice with the nutritionally complete diet, oocyte abnormalities were partially reverted, suggesting that vitamin B 1 deficiency acted by inhibiting oocytes meiotic maturation trough a reversible process [ 55 ].…”

Section: Agents Consideredmentioning

confidence: 99%

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Effects of Supplementation with Natural Antioxidants on Oocytes and Preimplantation Embryos

Budani

Tiboni

2020

Antioxidants

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For most infertile couples, in vitro fertilization (IVF) represents the only chance to conceive. Given the limited success of IVF procedures, novel approaches are continuously tested with the aim of improving IVF outcomes. Growing attention is devoted today to the potential benefit of natural antioxidants in the optimization of infertility treatments. This review summarizes current data in this context, focusing on both experimental studies on oocytes/embryos and clinical trials on antioxidants supplementation. Based on information gained from experimental studies, antioxidant supplementation may have beneficial effects on IVF outcomes in terms of quality and cryotolerance of in vitro produced embryos, together with positive effects on in vitro maturation oocytes and on early embryonic development. Unfortunately, from the clinical side, there is a paucity of evidence favoring the protective qualities of antioxidants. Among the antioxidants considered, coenzyme Q10 may be regarded as one of the most promising for its positive role in rescuing the oxidative stress-induced damages, but further data are needed. It is concluded that further trials are necessary to characterize the potential clinical value of antioxidants in IVF treatments.

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Section: Agents Consideredmentioning

confidence: 99%

Section: Agents Consideredmentioning

confidence: 99%

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Effects of Supplementation with Natural Antioxidants on Oocytes and Preimplantation Embryos

Budani

Tiboni

2020

Antioxidants

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“…Melatonin also inhibits angiogenesis via VEGF and oxidative stress via regulating radical scavenging activity and amplifies apoptotic activity via CASP3 mediated pathway in vivo and in vitro in EM 63,142,143 . Melatonin has no adverse effects on reproductive functions, instead, it can improve ovarian functions, and thus has potential to treat EM‐related infertility 296,297 . High‐dose intravenous treatment of pain and sepsis with melatonin showed no adverse effects 298 .…”

Section: Potential Pharmaceuticals With Multiple Targetsmentioning

confidence: 99%

Pharmaceuticals targeting signaling pathways of endometriosis as potential new medical treatment: A review

Hung

Zhang

Tan

et al. 2021

Medicinal Research Reviews

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Endometriosis (EM) is defined as endometrial tissues found outside the uterus. Growth and development of endometriotic cells in ectopic sites can be promoted via multiple pathways, including MAPK/MEK/ERK, PI3K/Akt/mTOR, NF‐κB, Rho/ROCK, reactive oxidative stress, tumor necrosis factor, transforming growth factor‐β, Wnt/β‐catenin, vascular endothelial growth factor, estrogen, and cytokines. The underlying pathophysiological mechanisms include proliferation, apoptosis, autophagy, migration, invasion, fibrosis, angiogenesis, oxidative stress, inflammation, and immune escape. Current medical treatments for EM are mainly hormonal and symptomatic, and thus the development of new, effective, and safe pharmaceuticals targeting specific molecular and signaling pathways is needed. Here, we systematically reviewed the literature focused on pharmaceuticals that specifically target the molecular and signaling pathways involved in the pathophysiology of EM. Potential drug targets, their upstream and downstream molecules with key aberrant signaling, and the regulatory mechanisms promoting the growth and development of endometriotic cells and tissues were discussed. Hormonal pharmaceuticals, including melatonin, exerts proapoptotic via regulating matrix metallopeptidase activity while nonhormonal pharmaceutical sorafenib exerts antiproliferative effect via MAPK/ERK pathway and antiangiogenesis activity via VEGF/VEGFR pathway. N‐acetyl cysteine, curcumin, and ginsenoside exert antioxidant and anti‐inflammatory effects via radical scavenging activity. Natural products have high efficacy with minimal side effects; for example, resveratrol and epigallocatechin gallate have multiple targets and provide synergistic efficacy to resolve the complexity of the pathophysiology of EM, showing promising efficacy in treating EM. Although new medical treatments are currently being developed, more detailed pharmacological studies and large sample size clinical trials are needed to confirm the efficacy and safety of these treatments in the near future.

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“…The ovary plays vital roles in female reproductive performance (Castagna et al, 2004;Gethoffer et al, 2018;Zhao et al, 2018) by promoting ovulation (exocrine function) and hormone secretion (endocrine function) (Sirotkin et al, 2018;Zangirolamo et al, 2018;Russell, 2019). The ovary is an ideal model for the study of ovarian biology and follicular development (Bahr, 1991), and the follicular granulosa cells (GCs) are often regarded as an important marker of follicular development because of their unique structural characteristics and important role in follicular development (Johnson and Woods, 2009).…”

Section: Introductionmentioning

confidence: 99%

High Expression of miR-204 in Chicken Atrophic Ovaries Promotes Granulosa Cell Apoptosis and Inhibits Autophagy

Cui

Liu

Amevor

et al. 2020

Front. Cell Dev. Biol.

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Chicken atrophic ovaries have decreased volume and are indicative of ovarian failure, presence of a tumor, or interrupted ovarian blood supply. Ovarian tumor is accompanied by an increase in follicular atresia, granulosa cell (GC) apoptosis, and autophagy. In a previous study, we found using high throughput sequencing that miR-204 is highly expressed in chicken atrophic ovaries. Thus, in the present study, we further investigated its function in GC apoptosis and autophagy. We found that overexpression of miR-204 reduced mRNA and protein levels of proliferation-related genes and increased apoptosis-related genes. Cell counting kit-8 (CCK-8), 5-ethynyl-2-deoxyuridine (EdU), and flow cytometry assays revealed that miR-204 inhibited GC proliferation and promoted apoptosis. Furthermore, we confirmed with reporter gene assays that Forkhead box K2 (FOXK2) was directly targeted by miR-204. FOXK2, as a downstream regulator of phosphoinositide 3-kinase (PI3K)/AKT/mammalian target of rapamycin (mTOR) signal pathways, promoted GC proliferation and inhibited apoptosis. Subsequently, we observed that miR-204 was involved in GC autophagy by targeting Transient Receptor Potential Melastatin 3 (TRPM3). The luciferase activities of the two binding sites of TRPM3 were decreased in response to treatment with a miR-204 mimic, and the autophagic flux was increased after miR-204 inhibition. However, overexpression of miR-204 had opposite results in autophagosomes and autolysosomes. miR-204 inhibits GC autophagy by suppressing the protein expression of TRPM3/AMP-activated protein kinase (AMPK)/ULK signaling pathway components. Inhibition of miR-204 enhanced autophagy by accumulating and degrading the protein levels of LC3-II (Microtubule Associated Protein Light Chain 3B) and p62 (Protein of 62 kDa), respectively, whereas miR-204 overexpression was associated with contrary results. Immunofluorescence staining showed that there was a significant reduction in the fluorescent intensity of LC3B, whereas p62 protein was increased after TRPM3 silencing. Collectively, our results indicate that miR-204 is highly expressed in chicken atrophic ovaries, which promotes GC apoptosis via repressing FOXK2 through the PI3K/AKT/mTOR pathway and inhibits autophagy by impeding the TRPM3/AMPK/ULK pathway.

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Female Reproductive Performance in the Mouse: Effect of Oral Melatonin

Cited by 12 publications

References 40 publications

Effects of Supplementation with Natural Antioxidants on Oocytes and Preimplantation Embryos

Effects of Supplementation with Natural Antioxidants on Oocytes and Preimplantation Embryos

Pharmaceuticals targeting signaling pathways of endometriosis as potential new medical treatment: A review

High Expression of miR-204 in Chicken Atrophic Ovaries Promotes Granulosa Cell Apoptosis and Inhibits Autophagy

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