Female fertility does not require Bmal1 in suprachiasmatic nucleus neurons expressing arginine vasopressin, vasoactive intestinal peptide, or neuromedin-S

Tonsfeldt, Karen J.; Cui, Laura; Lee, Jinkwon; Walbeek, Thijs J.; Brusman, Liza E.; Ye, Jin; Mieda, Michihiro; Gorman, Michael R.; Mellon, Pamela L.

doi:10.3389/fendo.2022.956169

Cited by 4 publications

(3 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the same mice exhibit more severe disturbance of wheel-running behavior rhythm resembling those described as “arrhythmic” or “split” in VPAC2 neuron-specific Bmal1 deletion ( 25 ). In addition, impaired circadian rhythms of home-cage activity and body temperature in Avp-Bmall -/- mice are strikingly similar to those of Nms-Bmal1 -/- mice ( 35 ). The latter has been reported to be arrhythmic in wheelrunning rhythm ( 34 ).…”

Section: Discussionmentioning

confidence: 79%

“…In contrast, Lee et al reported that mice with Bmal1 deletion in NMS neurons ( Nms-Bmal1 -/- mice) were arrhythmic in wheel-running behavior ( 34 ). Notably, a recent study showed that rhythms of home-cage activity and body temperature in Avp-Bmal1 -/- mice were very similar to those in Nms-Bmal1 -/- mice ( 35 ). Given the critical role of SCN AVP neurons in setting the free-running period, we re-evaluated the circadian behavior rhythm of Avp-Bmal1 -/- and Avp-CK1δ -/- mice by measuring wheel-running activity.…”

Section: Resultsmentioning

confidence: 90%

See 1 more Smart Citation

AVP neurons act as the primary circadian pacesetter cells in vivo

Tsuno

Peng

Horike

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

The central circadian clock of the suprachiasmatic nucleus (SCN) is a network consisting of various neurons and glia. Individual cells have the autonomous molecular machinery of a cellular clock, but their intrinsic periods are considerably variable. Here, we show that arginine vasopressin (AVP) neurons set the ensemble period of the SCN network to control circadian behavior rhythm. Artificial lengthening of cellular periods by deleting casein kinase 1 delta (CK1δ) in the whole SCN lengthened the free-running period of behavior rhythm to an extent similar to CK1δ deletion specific to AVP neurons. In SCN slices, PER2::LUC reporter rhythms of these mice did not recapitulate the period lengthening. However, in vivo calcium rhythms of both AVP and vasoactive intestinal peptide (VIP) neurons demonstrated lengthened periods similar to the behavioral rhythm upon AVP neuron-specific CK1δ deletion. These results indicate that AVP neurons act as the primary determinant of the SCN ensemble period.

show abstract

Section: Discussionmentioning

confidence: 79%

Section: Resultsmentioning

confidence: 90%

AVP neurons act as the primary circadian pacesetter cells in vivo

Tsuno

Peng

Horike

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…In contrast, Lee and colleagues reported that mice with Bmal1 deletion in NMS neurons ( Nms-Bmal1 −/− mice) lost the circadian rhythm in wheel-running behavior [ 21 ]. Notably, a recent study showed that rhythms of home-cage activity and body temperature in Avp-Bmal1 −/− mice were very similar to those in Nms-Bmal1 −/− mice [ 34 ]. Thus, circadian phenotypes may be significantly different between wheel-running and home-cage activity rhythms.…”

Section: Resultsmentioning

confidence: 99%

In vivo recording of suprachiasmatic nucleus dynamics reveals a dominant role of arginine vasopressin neurons in circadian pacesetting

Tsuno,

Peng,

Horike

et al. 2023

PLoS Biol

Self Cite

View full text Add to dashboard Cite

The central circadian clock of the suprachiasmatic nucleus (SCN) is a network consisting of various types of neurons and glial cells. Individual cells have the autonomous molecular machinery of a cellular clock, but their intrinsic periods vary considerably. Here, we show that arginine vasopressin (AVP) neurons set the ensemble period of the SCN network in vivo to control the circadian behavior rhythm. Artificial lengthening of cellular periods by deleting casein kinase 1 delta (CK1δ) in the whole SCN lengthened the free-running period of behavior rhythm to an extent similar to CK1δ deletion specific to AVP neurons. However, in SCN slices, PER2::LUC reporter rhythms of these mice only partially and transiently recapitulated the period lengthening, showing a dissociation between the SCN shell and core with a period instability in the shell. In contrast, in vivo calcium rhythms of both AVP and vasoactive intestinal peptide (VIP) neurons in the SCN of freely moving mice demonstrated stably lengthened periods similar to the behavioral rhythm upon AVP neuron-specific CK1δ deletion, without changing the phase relationships between each other. Furthermore, optogenetic activation of AVP neurons acutely induced calcium increase in VIP neurons in vivo. These results indicate that AVP neurons regulate other SCN neurons, such as VIP neurons, in vivo and thus act as a primary determinant of the SCN ensemble period.

show abstract