Mutations in MECP2 are associated with Rett syndrome, an X-linked neurodevelopmental disorder. To identify genes targeted by Mecp2, we sequenced 100 in vivo Mecp2-binding sites in mouse brain. Several sequences mapped to an imprinted gene cluster on chromosome 6, including Dlx5 and Dlx6, whose transcription was roughly two times greater in brains of Mecp2-null mice compared with those of wild-type mice. The maternally expressed gene DLX5 showed a loss of imprinting in lymphoblastoid cells from individuals with Rett syndrome. Because Dlx5 regulates production of enzymes that synthesize gamma-aminobutyric acid (GABA), loss of imprinting of Dlx5 may alter GABAergic neuron activity in individuals with Rett syndrome. In mouse brain, Dlx5 imprinting was relaxed, yet Mecp2-mediated silent-chromatin structure existed at the Dlx5-Dlx6 locus in brains of wild-type, but not Mecp2-null, mice. Mecp2 targeted histone deacetylase 1 to a sharply defined, approximately 1-kb region at the Dlx5-Dlx6 locus and promoted repressive histone methylation at Lys9 at this site. Chromatin immunoprecipitation-combined loop assays showed that Mecp2 mediated the silent chromatin-derived 11-kb chromatin loop at the Dlx5-Dlx6 locus. This loop was absent in chromatin of brains of Mecp2-null mice, and Dlx5-Dlx6 interacted with far distant sequences, forming distinct active chromatin-associated loops. These results show that formation of a silent-chromatin loop is a new mechanism underlying gene regulation by Mecp2.
Summary
Rare variants enriched for functions in chromatin regulation and neuronal
synapses have been linked to autism. How chromatin and DNA methylation interact with
environmental exposures at synaptic genes in autism etiologies is currently unclear. Using
whole genome bisulfite sequencing in brain tissue and a neuronal cell culture model
carrying a 15q11.2-q13.3 maternal duplication, we find significant global DNA
hypomethylation that is enriched over autism candidate genes and impacts gene expression.
The cumulative effect of multiple chromosomal duplications and exposure to the pervasive
persistent organic pollutant PCB 95 altered methylation of >1,000 genes.
Hypomethylated genes were enriched for H2A.Z, increased maternal UBE3A in
Dup15q corresponded to reduced levels of RING1B, and bivalently modified H2A.Z was altered
by PCB 95 and duplication. These results demonstrate the compounding effects of genetic
and environmental insults on the neuronal methylome that converge upon dysregulation of
chromatin and synaptic genes.
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