Feeding Response in
            <i>Aedes aegypti</i>
            : Stimulation by Adenosine Triphosphate

Galun, Rachel; Avi‐Dor, Y.; Bar-Zeev, M.

doi:10.1126/science.142.3600.1674

Cited by 117 publications

(55 citation statements)

References 6 publications

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“…Thus it seems that in addition to assessing osmotic pressure, hematophagous insects also specifically require sodium for optimal feeding responses (Galun et al, 1963). In our study, much greater engorgement responses were observed when bugs were offered a PBS solution rather than water alone, indicating that bed bugs might be stimulated by one or more of the constituent salts.…”

Section: Research Articlementioning

confidence: 54%

“…A decrease in feeding due to reduction of phosphate groups was also reported in the hematophagous triatomine Rhodnius prolixus (Smith and Friend, 1976) and the mosquito A. aegypti (Galun et al, 1963). Galun et al (Galun et al, 1963) demonstrated that adenosine tetraphosphate was even more effective than ATP, indicating that the presence of the adenine moiety and number of phosphate groups are important stimuli of the chemosensory system involved in assessing blood quality and engorgement. Moreover, the 5′ position of the phosphates on the ribose was critical for phagostimulatory activity (Galun, 1967).…”

Section: Discussionmentioning

confidence: 98%

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Blood constituents as phagostimulants for the bed bug,Cimex lectularius L

Romero

Schal

2013

Journal of Experimental Biology

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Many hematophagous arthropods are stimulated by blood constituents to initiate feeding. We used a membrane-based feeding system to identify chemicals that stimulate acceptance and engorgement responses in various life stages of bed bugs. Water was fortified with a variety of compounds (e.g. salts, amino acids, vitamins, nucleotides, cholesterol and fatty acids) in these bioassays. ATP was the most effective phagostimulant in adults and nymphs, resulting in >70% of bed bugs fully engorging. Addition of NaCl to low ATP solutions that alone elicited <50% engorgement significantly enhanced feeding responses of bed bugs. A comparison of feeding responses with solutions of various adenine nucleotides showed that ATP was more stimulatory than ADP, which was more effective than AMP. Feeding assays with physiological levels of other blood constituents such as D-glucose, albumin, globulin, cholesterol and mixtures of vitamins and amino acids did not stimulate engorgement, suggesting that adenine nucleotides are the most important feeding stimulants in bed bugs. Identification of phagostimulants for bed bugs will contribute towards the development of artificial diets for rearing purposes, as well as for the development of alternative methods to eliminate bed bug infestations.

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Section: Research Articlementioning

confidence: 54%

Section: Discussionmentioning

confidence: 98%

Blood constituents as phagostimulants for the bed bug,Cimex lectularius L

Romero

Schal

2013

Journal of Experimental Biology

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“…Females (3-4 days old) were blood-fed on anesthetized mice. Protein-free meals consisted of latex beads (LB-5, Sigma;Billingsley and Rudin 1992) suspended in 150 mM NaCl, 10 mM NaHCO 3 pH 7.0 (Moskalyk et al 1996), with 1 mM ATP being added as a phagostimulant (Galun et al 1963). …”

Section: Mosquitoesmentioning

confidence: 99%

Storage and secretion of Ag-Aper14, a novel peritrophic matrix protein, and Ag-Muc1 from the mosquito Anopheles gambiae

et al. 2005

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The gene Ag-Aper14 encodes a novel peritrophic matrix (or peritrophic membrane; PM) protein in the mosquito Anopheles gambiae. The Ag-Aper14 protein is merely 89 amino acids long and has a single putative chitin-binding domain. Prior to blood feeding, the Ag-Aper14 protein is stored in secretory vesicles next to the epithelial cell lumenal surface. Immunoelectron microscopy has revealed that Ag-Aper14 co-localizes to the same secretory vesicles as another PM protein, Ag-Aper1, indicating a common mode of regulated secretion. Conversely, Ag-Muc1, an epithelial cell-surface protein, does not co-localize to these secretory vesicles and is detected only on the cell surface. After blood feeding, Ag-Aper14 is secreted and incorporated into the PM that surrounds the ingested blood.

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“…Stimulation of feeding response in Ae. aegypti and other bloodfeeding insect species by ATP was first described by Galun et al (1963), and the phenomenon was studied extensively by Galun and others (Friend 1978, Smith andFriend 1982). In an electrophysiological study with Ae.…”

Section: Introductionmentioning

confidence: 97%

A Quantitative In Vitro Assay for Chemical Mosquito-Deterrent Activity without Human Blood Cells¹

Klun¹,

Kramer²,

Zhang³

et al. 2008

Journal of the American Mosquito Control Association

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We report that an aqueous solution containing 10(-3) M adenosine triphosphate (ATP) and citrate-phosphate-dextrose-adenine (CPDA-1) can effectively replace transfusable human red blood cells in an in vitro Klun and Debboun bioassay system for evaluating chemicals for mosquito feeding-deterrent activity, using either Aedes aegypti or Anopheles stephensi. These species fed with similar avidity through collagen membrane covering aqueous 10(-3) M ATP plus CPDA-1 or red blood cells in CPDA-1 supplemented with ATP. In a 2nd experiment, we evaluated the feeding-deterrent activity of N,N-diethyl-3-methylbenzamide and a newly discovered natural product chemical, (-)-isolongifolenone, against these 2 mosquito species. We found that the feeding-deterrent efficacy of the 2 chemicals was similar whether the feeding stimulant was red blood cells supplemented with ATP or ATP alone with CPDA-1. Since the use of human red blood cells in bioassays raises important health and logistic issues, aqueous ATP with CPDA-1 is a reasonable alternative to human blood cells for routine in vitro chemical screening.

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Feeding Response in Aedes aegypti : Stimulation by Adenosine Triphosphate

Cited by 117 publications

References 6 publications

Blood constituents as phagostimulants for the bed bug,Cimex lectularius L

Blood constituents as phagostimulants for the bed bug,Cimex lectularius L

Storage and secretion of Ag-Aper14, a novel peritrophic matrix protein, and Ag-Muc1 from the mosquito Anopheles gambiae

A Quantitative In Vitro Assay for Chemical Mosquito-Deterrent Activity without Human Blood Cells¹

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Feeding Response in Aedes aegypti : Stimulation by Adenosine Triphosphate

Cited by 117 publications

References 6 publications

Blood constituents as phagostimulants for the bed bug,Cimex lectularius L

Blood constituents as phagostimulants for the bed bug,Cimex lectularius L

Storage and secretion of Ag-Aper14, a novel peritrophic matrix protein, and Ag-Muc1 from the mosquito Anopheles gambiae

A Quantitative In Vitro Assay for Chemical Mosquito-Deterrent Activity without Human Blood Cells1

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Product

Resources

About

A Quantitative In Vitro Assay for Chemical Mosquito-Deterrent Activity without Human Blood Cells¹