A Quantitative In Vitro Assay for Chemical Mosquito-Deterrent Activity without Human Blood Cells<sup>1</sup>

Klun, J. A.; Kramer, Matthew; Zhang, Aijun; Wang, Shifa; Debboun, Mustapha

doi:10.2987/08-5755.1

Cited by 19 publications

(16 citation statements)

References 10 publications

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“…Briefly the assay system consists of a six well blood reservoir with each of the 3 cm X 4 cm wells containing 6 mL of blood. As reported earlier,26 female mosquitoes feed as well on the CPDA −1 + ATP as they do on blood. Therefore, we used the CPDA-1 + ATP instead of blood.…”

Section: Methodssupporting

confidence: 73%

“…CPDA-1 and ATP preparations were freshly mixed on the day of the test. CPDA-1 + ATP is reported to be as attractive to mosquito females for feeding as blood 26. DEET (N,N-diethyl- m -toluamide 97%) was obtained from Sigma Eldrich (St. Louis, MO).…”

Section: Methodsmentioning

confidence: 99%

“…Experiments were conducted by using a six-celled in vitro Klun & Debboun (K & D) module bioassay system developed by Klun et al26 for quantitative evaluation of bite deterrent properties of candidate compounds for human use. This bioassay method determines specifically measured biting (feeding) deterrent properties of the chemicals.…”

Section: Methodsmentioning

confidence: 99%

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Synthesis of (−)-callicarpenal, a potent arthropod repellent

Ling

SMITH

et al. 2011

Tetrahedron

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Callicarpenal (1), a natural terpenoid isolated from American beautyberry (Callicarpa americana), has shown significant repellent activities against mosquitoes, ticks and imported fire ants. Here we report our efficient synthetic approach to this natural product, and preliminary results of the mosquito biting-deterrent effects of callicarpenal as well as its synthetic precursors and related C 8 -epimers. The synthetic strategy allows rapid access to various epimers and analogues of the natural product that can be used to explore its structure-activity relationship and optimize its biological properties.

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Section: Methodssupporting

confidence: 73%

Section: Methodsmentioning

confidence: 99%

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Synthesis of (−)-callicarpenal, a potent arthropod repellent

Ling

SMITH

et al. 2011

Tetrahedron

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“…Numeric estimates of power in a similar testing environment, also using K&D modules, along with details not discussed here, are given in Klun et al (2008). Our results from investigating the power of tests shows that, for the same number of mosquitoes, the K&D module will have more power to determine whether a substance is toxic than whether it is repellent.…”

Section: Discussionmentioning

confidence: 86%

Mosquito Biting Behavior: Statistical Power and Sources of Variation in Toxicity and Repellent Bioassays

Kramer¹,

Feldlaufer²,

Chauhan³

2010

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Compounds thought to be effective against mosquitoes as either 'insecticides' or 'repellents' have recently been shown to contain properties of both, or possess other behavior-modifying actions. Prompted in part by these reports, we conducted posterior analyses of our data to examine some interrelated statistical issues inherent in our bioassay system. Using a modified K&D module, the responses of over 25,000 adult Aedes aegypti (L.) females exposed to either alphacypermethrin or DEET were compared with the responses of mosquitoes exposed to untreated controls for toxicity and biting (alphacypermethrin) or biting alone (DEET). Our analyses indicated that; (1) our bioassay system has more statistical power to determine a compound's toxicity than its repellent qualities, (2) day-to-day variability is large and needs to be accommodated in analyses; there are other, potentially even larger sources of variability (e.g., mosquito heterogeneity) which invalidate statistical tests that are based on the assumption of binomially or multinomially distributed data (e.g., chi2 tests), and (3) unlike biting mosquitoes exposed to DEET, the proportions of biting mosquitoes exposed to alphacypermethrin are unrelated to the proportions of concurrently tested biting controls, even after adjusting for daily variation in toxicity. Thus, there is a clear behavioral indicator in this bioassay system that the 'repellency' of DEET (a presumed repellent) differs in a fundamental way from that of alphacypermethrin (a presumed toxicant), which may allow the differentiation between classes of compounds based on biting behavior alone.

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“…Recently, isolongifolenone, obtained through the oxidation of isolongifolene which was isomerized by natural product of longifolene , has been found to exert potent against tyrosinase and breast cancer . Besides, isolongifolenone was superior to DEET in repelling ticks and deterring feeding mosquitoes . However, up to date, isolongifolenone oxime ethers or esters derivatives have not been reported.…”

Section: Introductionmentioning

confidence: 99%

Design, Synthesis, and Bioassay of Novel Compounds of Isolongifolenone Oxime Derivatives

Zhang

et al. 2016

Helvetica Chimica Acta

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A succession of new isolongifolenone oxime derivatives have been designed and synthesized. The structures of these compounds were identified by IR, 1H‐NMR, 13C‐NMR, mass spectra, and elemental analysis. The bioassays of antibacterial, antifungal, and insecticidal activity were carried out. The in vitro antibacterial and antifungal activities were evaluated by the disk diffusion method, and the minimum inhibitory concentration was determined by the microdilution method, while the insecticidal activity was tested by the spraying method or the straw impregnation method. The results of bioassays showed that compound 3f was more active in resisting all the tested bacterial and fungal organisms when compared to the standard drug amoxicillin at the lowest concentration of 31.3 μg/ml. Compound 4, synthesized by Beckmann rearrangement reaction of isolongifone oxime, exerted moderate insecticidal activity against soybean aphid. Furthermore, compound 3m exhibited more activity in killing armyworms than the standard drug flucycloxuron at the concentration of 0.5 mg/l.

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A Quantitative In Vitro Assay for Chemical Mosquito-Deterrent Activity without Human Blood Cells¹

Cited by 19 publications

References 10 publications

Synthesis of (−)-callicarpenal, a potent arthropod repellent

Synthesis of (−)-callicarpenal, a potent arthropod repellent

Mosquito Biting Behavior: Statistical Power and Sources of Variation in Toxicity and Repellent Bioassays

Design, Synthesis, and Bioassay of Novel Compounds of Isolongifolenone Oxime Derivatives

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A Quantitative In Vitro Assay for Chemical Mosquito-Deterrent Activity without Human Blood Cells1

Cited by 19 publications

References 10 publications

Synthesis of (−)-callicarpenal, a potent arthropod repellent

Synthesis of (−)-callicarpenal, a potent arthropod repellent

Mosquito Biting Behavior: Statistical Power and Sources of Variation in Toxicity and Repellent Bioassays

Design, Synthesis, and Bioassay of Novel Compounds of Isolongifolenone Oxime Derivatives

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Product

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A Quantitative In Vitro Assay for Chemical Mosquito-Deterrent Activity without Human Blood Cells¹