Feeding milk replacer instead of whole milk affects blood plasma proteome and lipid profile in preruminant calves

Lepczyński, Adam; Herosimczyk, Agnieszka; Ożgo, M.; Skrzypczak, W. F.

doi:10.1515/pjvs-2015-0012

Cited by 4 publications

(3 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lepczyński et al (17) observed lower blood plasma concentrations of total cholesterol, HDL-cholesterol, and LDL-cholesterol and downregulation of apo A-IV in preruminant calves fed milk replacer in comparison to calves fed whole milk. Moreover, the authors showed that the milk replacer had a much higher carbohydrate content than the milk (17). Apolipoprotein A-IV is a glycoprotein synthesized primarily in the enterocytes of the small intestine during fat absorption.…”

Section: Discussionmentioning

confidence: 99%

Lactose-rich milk replacer modifies the proteome of bloodplasma in 2-week-old calves

Chalupnik¹,

Ożgo²,

Herosimczyk³

et al. 2016

Turk J Vet Anim Sci

View full text Add to dashboard Cite

The aim of the study was to create a specific pattern of protein expression changes in the plasma of 2-week-old calves treated with excessive lactose doses. Identification of proteins, the expression of which changes during the short-term administration of lactose, may help to exclude or confirm the causes of diarrhea observed in newborn calves. The present study utilized 2-D electrophoresis combined with MALDI-TOF mass spectrometry and identified 6 blood plasma proteins, the expression of which changed under the influence of lactose added to the milk replacer. The pattern of expression of blood plasma proteins, including decreased expression of fibrinogen and apolipoprotein A-IV and increased expression of alpha-1B-glycoprotein, alpha-1-antiproteinase, and apolipoprotein E in 2-week-old calves fed with lactose-rich milk replacer, can be used to determine the causes of diarrhea in calves.

show abstract

Section: Discussionmentioning

confidence: 99%

Lactose-rich milk replacer modifies the proteome of bloodplasma in 2-week-old calves

Chalupnik¹,

Ożgo²,

Herosimczyk³

et al. 2016

Turk J Vet Anim Sci

View full text Add to dashboard Cite

show abstract

“…Previous proteomic studies of calf blood plasma [30] have shown a significant reduction in apoA-IV expression on the first day, when changing from milk feeding to milk replacer. During the next days of life, the expression of this protein increased.…”

Section: Discussionmentioning

confidence: 95%

Urinary Proteome of Newborn Calves—New Potential in Non-Invasive Neonatal Diagnostic

Dratwa-Chałupnik

Wojdyła

Ożgo

et al. 2020

Animals

View full text Add to dashboard Cite

Urine is a biological diagnostic material suitable not only for the analysis of kidney and urinary tract functions but also the function of other tissues and organs. The urine proteome of adult mammals differs from the urine proteome of neonatal ones. The establishment of urinary protein maps of healthy newborn calves is important for diagnosing and monitoring the progression of various diseases. The experiment was carried out on a Polish-Friesian var. of Black-and-White male calves in the sixth day of postnatal life. The two proteomics approaches used for separation and identification of urinary proteins were: 2-DE with MALDI-TOF-TOF-MS/MS and 1-DE with LC-MS/MS. This resulted in the identification of 692 urinary proteins. The majority of them were classified as extracellular proteins (40.32%), as well as proteins involved in regulation of major cellular processes (31.07%). We have observed the presence of unique proteins associated with embryonic (ameloblastin, alpha-fetoprotein, Delta-like protein, embryo-specific fibronectin 1 transcript variant, Indian hedgehog homolog) and kidney development (angiotensin-converting enzyme, angiotensinogen, aquaporin-1, calbindin, glypican 3, nidogen 1, pro-cathepsin H). Additionally, proteins involved in the renal regulation of water and electrolyte balance (angiotensinogen, angiotensin-converting enzyme, aquaporin-1, ezrin, uromodulin) were detected. Presented in the current study 1-D and 2-D urinary proteomic maps are the basis for the identification and detection of prognostic biomarkers important for defining a calf’s health status.

show abstract

“…So, animals fed with milk have a high concentration of cholesterol, low and high density lipoproteins in the blood plasma. At the same time, the composition of the milk replacer as a fat source is the main factor influencing the synthesis of proteins important for cholesterol metabolism and the lipid profile in artificially fed calves (Lepczyński et al, 2015;Ross et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Lipid composition of blood plasma and epithelium of the jejunal mucosa in calves with dyspepsia and its correction

Gryshchenko,

Danchenko,

Tkachuk

et al. 2023

Regul. Mech. Biosyst.

View full text Add to dashboard Cite

Enteropathology in newborn ruminants significantly affects the functional formation of the digestive tract, which induces complications in other organs and systems, reducing the resistance and productivity in recovered animals. It is also characterized by metabolic disorders, particularly lipids, which adversely affects the regenerative processes at the cellular level. The material for the study was 2-day-old calves, from which 3 groups of 6 animals each were formed. 1st group – control was made up of clinically healthy animals, 2nd group – calves with toxic dyspepsia which received traditional treatment and 3rd group – calves which were additionally orally administered a liposomal form of a 1% solution of BAD "FLP-MD". The lipid composition of blood plasma and the epithelium of the mucous membrane of the jejunum of calves was studied by the method of thin-layer chromatography on standard Silufol plates. The lipidogram of blood plasma in 30-day-old calves recovered from dyspepsia under traditional treatment regimen was characterized by dyslipidemia, which was manifested by an increase in total lipid content by 10.0% amid hypercholesterolemia and hypertriacylglycerolemia and a decrease in free fatty acids by 24.6% and phospholipids by 11.8%. Also, phosphatidylcholine and sphingomyelin content decreasesd in the blood plasma. In epithelium of the jejunal mucosa, total lipid content decreased due to total cholesterol, triacylglycerols, and phospholipids. The restoration of total lipid content and individual fractions was found in blood plasma of calves that received additionally phospholipid-containing bio-additive "FLP-MD". Among the individual phospholipids phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine prevailed. In these calves, most of the lipid components in the epithelium of the jejunal mucosa were stabilized. In the future, we plan to investigate the content of total lipids in the blood and mucous membrane of the small intestine of calves.

show abstract

Feeding milk replacer instead of whole milk affects blood plasma proteome and lipid profile in preruminant calves

Cited by 4 publications

References 26 publications

Lactose-rich milk replacer modifies the proteome of bloodplasma in 2-week-old calves

Lactose-rich milk replacer modifies the proteome of bloodplasma in 2-week-old calves

Urinary Proteome of Newborn Calves—New Potential in Non-Invasive Neonatal Diagnostic

Lipid composition of blood plasma and epithelium of the jejunal mucosa in calves with dyspepsia and its correction

Contact Info

Product

Resources

About