Fed-batch fermentation of mixed carbon source significantly enhances the production of docosahexaenoic acid in Thraustochytriidae sp. PKU#Mn16 by differentially regulating fatty acids biosynthetic pathways

Ye, Huike; He, Yaodong; Xie, Yunxuan; Sen, Biswarup; Wang, Guangyi

doi:10.1016/j.biortech.2019.122402

Cited by 39 publications

(16 citation statements)

References 36 publications

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“…The mixed substrate of glucose and glycerol by fed-batch fermentation with Thraustochytriidae sp. PKU#Mn16 using produced DHA yield of 8.65% and productivity of 100.7 ± 2.9 mg/L/h [ 132 ].…”

Section: Glycerol Into Value-added Productsmentioning

confidence: 99%

Glycerol waste to value added products and its potential applications

Chilakamarry

Sakinah

Zularisam

et al. 2021

Syst Microbiol and Biomanuf

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Graphic abstract The rapid industrial and economic development runs on fossil fuel and other energy sources. Limited oil reserves, environmental issues, and high transportation costs lead towards carbon unbiased renewable and sustainable fuel. Compared to other carbon-based fuels, biodiesel is attracted worldwide as a biofuel for the reduction of global dependence on fossil fuels and the greenhouse effect. During biodiesel production, approximately 10% of glycerol is formed in the transesterification process in a biodiesel plant. The ditching of crude glycerol is important as it contains salt, free fatty acids, and methanol that cause contamination of soil and creates environmental challenges for researchers. However, the excessive cost of crude glycerol refining and market capacity encourage the biodiesel industries for developing a new idea for utilising and produced extra sources of income and treat biodiesel waste. This review focuses on the significance of crude glycerol in the value-added utilisation and conversion to bioethanol by a fermentation process and describes the opportunities of glycerol in various applications.

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Section: Glycerol Into Value-added Productsmentioning

confidence: 99%

Glycerol waste to value added products and its potential applications

Chilakamarry

Sakinah

Zularisam

et al. 2021

Syst Microbiol and Biomanuf

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“…The DHA biosynthesis pathway in Thraustochytriu has not been fully elucidated. It has been reported that the two pathways, i.e., The PKS system and FAS pathway, they are likely to be present [69,70]. In general, eukaryotes biosynthesize the polyunsaturated fatty acids through a series of desaturation and elongation reactions catalyzed by membrane-bound enzymes such as desaturases and elongases, known as the fatty acid synthetase (FAS) system.…”

Section: Pks Pathwaymentioning

confidence: 99%

Comparative Transcriptomic Analysis Uncovers Genes Responsible for the DHA Enhancement in the Mutant Aurantiochytrium sp.

Liu

et al. 2020

Microorganisms

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Docosahexaenoic acid (DHA), a n-3 long-chain polyunsaturated fatty acid, is critical for physiological activities of the human body. Marine eukaryote Aurantiochytrium sp. is considered a promising source for DHA production. Mutational studies have shown that ultraviolet (UV) irradiation (50 W, 30 s) could be utilized as a breeding strategy for obtaining high-yield DHA-producing Aurantiochytrium sp. After UV irradiation (50 W, 30 s), the mutant strain X2 which shows enhanced lipid (1.79-fold, 1417.37 mg/L) and DHA (1.90-fold, 624.93 mg/L) production, was selected from the wild Aurantiochytrium sp. Instead of eicosapentaenoic acid (EPA), 9.07% of docosapentaenoic acid (DPA) was observed in the mutant strain X2. The comparative transcriptomic analysis showed that in both wild type and mutant strain, the fatty acid synthesis (FAS) pathway was incomplete with key desaturases, but genes related to the polyketide synthase (PKS) pathway were observed. Results presented that mRNA expression levels of CoAT, AT, ER, DH, and MT down-regulated in wild type but up-regulated in mutant strain X2, corresponding to the increased intercellular DHA accumulation. These findings indicated that CoAT, AT, ER, DH, and MT can be exploited for high DHA yields in Aurantiochytrium.

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“…The DHA biosynthesis pathway in Thraustochytriu has not been fully elucidated. It has been reported that the two pathways, i.e., The PKS system and FAS pathway are likely to be present [32,33]. The commonly found extended products of FAS in nearly all organisms are long-chain saturated FAs of either C16 or C18 [34,35].…”

Section: Pks Pathwaymentioning

confidence: 99%

Mechanism study on the enhanced DHA synthesis in the mutant Thraustochytriidae sp. through comparative transcriptomic analysis

liu¹,

Hu²,

Li³

et al. 2020

Preprint

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Background: Docosahexaenoic acid (DHA) is an essential omega-3 fatty acid for the human retina, skin, and cerebral cortex. Marine eukaryote Thraustochytriidae sp. was considered as a promising source for the n -3 LC-PUFAs production. However, the mechanism how the LC-PUFAs was synthesized in Thraustochytriidae sp. still remained unclarified. To explore the vital genes responsible for the DHA enrichment, the functional transcriptomic annotation was compared between the wild type and preeminent mutant of Thraustochytriidae sp. X2. Results: After the UV irradiation (50 W, 30 s), the mutant X2 showed enhanced lipid (78.88 % more) and DHA (23.77 % more) production compared with the wild type. Instead of EPA, 9.07 % of DPA was observed in the mutant X2. The comparative transcriptomic analysis showed that in both wild type and mutant strain, FAS was incomplete and lacked key desaturases, but genes related to the PKS pathway were observed. It was oberved that mRNA expression levels of CoA-transferase (CoAT) , acyltransferase (AT), enoyl reductase (ER) , dehydratase (DH) and methyltransferase (MT) down-regulated in wild type but up-regulated in mutant X2, corresponding to the increased intercellular DHA accumulation. Conclusion: These findings indicated the potential genes that can be exploited for high DHA yields in Thraustochytriidae sp..

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Fed-batch fermentation of mixed carbon source significantly enhances the production of docosahexaenoic acid in Thraustochytriidae sp. PKU#Mn16 by differentially regulating fatty acids biosynthetic pathways

Cited by 39 publications

References 36 publications

Glycerol waste to value added products and its potential applications

Glycerol waste to value added products and its potential applications

Comparative Transcriptomic Analysis Uncovers Genes Responsible for the DHA Enhancement in the Mutant Aurantiochytrium sp.

Mechanism study on the enhanced DHA synthesis in the mutant Thraustochytriidae sp. through comparative transcriptomic analysis

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Fed-batch fermentation of mixed carbon source significantly enhances the production of docosahexaenoic acid in Thraustochytriidae sp. PKU#Mn16 by differentially regulating fatty acids biosynthetic pathways

Cited by 39 publications

References 36 publications

Glycerol waste to value added products and its potential applications

Glycerol waste to value added products and its potential applications

Comparative Transcriptomic Analysis Uncovers Genes Responsible for the DHA Enhancement in the Mutant Aurantiochytrium sp.

Mechanism study&nbsp;on the enhanced DHA synthesis in the mutant&nbsp;Thraustochytriidae sp.&nbsp;through comparative transcriptomic analysis

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Mechanism study on the enhanced DHA synthesis in the mutant Thraustochytriidae sp. through comparative transcriptomic analysis