Comparative Transcriptomic Analysis Uncovers Genes Responsible for the DHA Enhancement in the Mutant Aurantiochytrium sp.

Liu, Liangxu; Hu, Zhangli; Li, Shuangfei; Yang, Hao; Li, Siting; Lv, Chuhan; Zaynab, Madiha; Cheng, Christopher H.K.; Chen, Huapu; Yang, Xiao

doi:10.3390/microorganisms8040529

Cited by 27 publications

(11 citation statements)

References 77 publications

(94 reference statements)

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“…DHA is a n-3 long-chain polyunsaturated fatty acid and is critical for cellular processes involved in maintaining health. In the study of Liu et al (2020) [13], UV mutagenesis was utilized to obtain competitive Aurantiochytrium sp. strain with enhanced DHA production (1.90-fold higher than wild strain).…”

Section: Increasing Knowledge On Bioactive Propertiesmentioning

confidence: 99%

Microbial Secondary Metabolism and Biotechnology

Fouillaud

Dufossé

2022

Microorganisms

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In recent decades scientific research has demonstrated that the microbial world is infinitely richer and more surprising than we could have imagined. Every day, new molecules produced by microorganisms are discovered, and their incredible diversity has not yet delivered all of its messages. The current challenge of research is to select from the wide variety of characterized microorganisms and compounds, those which could provide rapid answers to crucial questions about human or animal health or more generally relating to society’s demands for medicine, pharmacology, nutrition or everyday well-being.

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Section: Increasing Knowledge On Bioactive Propertiesmentioning

confidence: 99%

Microbial Secondary Metabolism and Biotechnology

Fouillaud

Dufossé

2022

Microorganisms

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“…SZU445 was obtained by UV mutagenesis of Aurantiochytrium sp. PKU#Mn16 (Liu et al, 2020). The culture conditions were same as those in our previously reported study (Zhu et al, 2020).…”

Section: Strain Cultivationmentioning

confidence: 99%

“…The sequence of Aurantiochytrium sp. SZU445 18s rDNA (GenBank: MT232522.1) (Liu et al, 2020;Zhu et al, 2020) was amplified with the primer pair 18S-F/18S-R. The sequence of DECR was amplified from Aurantiochytrium sp.…”

Section: Plasmid Construction and Cloningmentioning

confidence: 99%

“…The cultured cells were collected by centrifugation at 6000 rpm for 10 min followed by freeze-drying for 72 h using a freeze dryer (10N, SCIENTZ, Ningbo, China) (Liu et al, 2020). Then, 500 mg of freeze-dried cells was extracted with chloroform: methanol (ratio 2:1 v/v) in a Soxhlet extractor (AG-SXT-06, OUGE, Shanghai, China) at 65°C for 60 h. After extraction, the samples were prepared according to our previously reported method .…”

Section: Fatty Acids Yield and Composition Analysismentioning

confidence: 99%

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Metabolic Engineering to Improve Docosahexaenoic Acid Production in Marine Protist Aurantiochytrium sp. by Disrupting 2,4-Dienoyl-CoA Reductase

et al. 2022

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Docosahexaenoic acid (DHA) has attracted attention from researchers because of its pharmacological and nutritional importance. Currently, DHA production costs are high due to fermentation inefficiency; however, improving DHA yield by metabolic engineering in thraustochytrids is one approach to reduce these costs. In this study, a high-yielding (53.97% of total fatty acids) DHA production strain was constructed by disrupting polyunsaturated fatty acid beta-oxidation via knockout of the 2,4-dienyl-CoA reductase (DECR) gene (KO strain) in Aurantiochytrium sp. Slight differences in cell growth was observed in the wild-type and transformants (OE and KO), with cell concentrations in stationary of 2.65×106, 2.36×106 and 2.56×106 cells mL-1 respectively. Impressively, the KO strain yielded 21.62% more neutral lipids and 57.34% greater DHA production; moreover, the opposite was observed when overexpressing DECR (OE strain), with significant decreases of 30.49% and 64.61%, respectively. Furthermore, the KO strain showed a prolonged DHA production period with a sustainable increase from 63 to 90 h (170.03 to 203.27 mg g−1 DCW), while that of the wildtype strain decreased significantly from 150.58 to 140.10 mg g−1 DCW. This new approach provides an advanced proxy for the construction of sustainable DHA production strains for industrial purposes and deepens our understanding of the metabolic pathways of Aurantiochytrium sp.

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“…To date, transcriptomic studies have been conducted on A. limacinum and other thraustochytrids (Dellero et al, 2020; Heggeset et al, 2019; Liang et al, 2018; Liu et al, 2020; Ma et al, 2015; Ye et al, 2019). However, most of these studies have primarily focused on the transcriptional profiles of genes related to lipid or PUFA biosynthesis pathways (Heggeset et al, 2019; Liang et al, 2018; Liu et al, 2020; Ma et al, 2015). Therefore, we are still far from a complete understanding of how A. limacinum regulates the production of astaxanthin and other carotenoids (Ye et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional responses of Aurantiochytrium limacinum under light conditions

Kubo

Morimoto

Shiroi

et al. 2022

Journal of Applied Microbiology

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Aims: Astaxanthin-producing protist Aurantiochytrium limacinum can accumulate higher amounts of astaxanthin under light conditions; however, little is known about the impact of light exposure on its metabolism. Here, we investigated the transcriptional profile of A. limacinum under light conditions. Methods and Results: Transcriptomic analyses revealed that 962 genes of A. limacinum showed a significant change in expression under light conditions, most of which (94.5%) were downregulated. Furthermore, gene ontology enrichment analysis indicated that A. limacinum mainly downregulated genes associated with cell motility, proliferation and gene expression processes, whose activities depend on ATP as an energy source. Additionally, the quantification of carotenoid and its transcripts suggested that βcarotene and astaxanthin biosynthesis pathways were rate-limiting and tightly regulated steps, respectively. In comparison, these processes were enhanced under light conditions. Conclusions:Considering that astaxanthin accumulation was highly correlated with reactive oxygen species (ROS) levels in microalgae, our results suggest that A. limacinum reduces ATP consumption to decrease the occurrence of ROS in mitochondria while accumulating astaxanthin to prevent ROS damage. Significance and Impact of Study:This study provides novel insights into the impact of light exposure on A. limacinum metabolism, thereby facilitating a complete understanding of this protist for efficient astaxanthin production.

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Comparative Transcriptomic Analysis Uncovers Genes Responsible for the DHA Enhancement in the Mutant Aurantiochytrium sp.

Cited by 27 publications

References 77 publications

Microbial Secondary Metabolism and Biotechnology

Microbial Secondary Metabolism and Biotechnology

Metabolic Engineering to Improve Docosahexaenoic Acid Production in Marine Protist Aurantiochytrium sp. by Disrupting 2,4-Dienoyl-CoA Reductase

Transcriptional responses of Aurantiochytrium limacinum under light conditions

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