Fecal Microbiota in Patients with Irritable Bowel Syndrome Compared with Healthy Controls Using Real-Time Polymerase Chain Reaction: An Evidence of Dysbiosis

Shukla, Ratnakar; Ghoshal, Ujjala; Dhole, Tapan N.; Ghoshal, Uday C

doi:10.1007/s10620-015-3607-y

Cited by 114 publications

(105 citation statements)

References 50 publications

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“…Several studies found a decrease of Lactobacilli spp. in faeces and mucosa of IBS patients [28–30], while, interventional trials demonstrated the capability of Lactobacilli to improve symptoms and modulate the inflammatory response in these patients [10]. However, to the best of our knowledge, no data are available on the effect of LC-DG on the mucosal inflammatory response.…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus casei DG and its postbiotic reduce the inflammatory mucosal response: an ex-vivo organ culture model of post-infectious irritable bowel syndrome

Compare¹,

Rocco²,

Coccoli³

et al. 2017

BMC Gastroenterol

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BackgroundThe evidence on the role of gut microbiota in post-infectious irritable bowel syndrome (PI-IBS) is convincing. Lactobacillus spp. positively affect IBS symptoms, although the mechanisms through which probiotics exert their beneficial effects are largely unknown. The aim of the study is to evaluate the role of Lactobacillus casei DG (LC-DG) and its postbiotic (PB) in modulating the inflammatory/immune-response in PI-IBS in an ex-vivo organ culture model.MethodsEx vivo cultures of ileal and colonic mucosa from 10 PI-IBS, diarrhea predominant subtype (D) patients, and 10 healthy controls (HC) were treated with LPS, LC-DG and PB. Interleukin (IL)-1α, IL-6, IL-8 and IL-10 mRNA levels were assessed by real-time PCR and Toll like receptor 4 (TLR-4) protein expression by Western blotting.ResultsAt baseline, IL-1α, IL-6 and IL-8 mRNA levels as well as TLR-4 protein expression were significantly higher while IL-10 mRNA levels were lower in PI-IBS D than in HC in both ileum and colon. LC-DG and PB significantly reduced the mRNA levels of pro-inflammatory cytokines and TLR-4 while increased that of IL-10 after LPS stimulation. The protective effect was more pronounced for PB than LC-DG treatment.ConclusionLC-DG and its PB attenuate the inflammatory mucosal response in an ex-vivo organ culture model of PI-IBS D.

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Section: Discussionmentioning

confidence: 99%

Lactobacillus casei DG and its postbiotic reduce the inflammatory mucosal response: an ex-vivo organ culture model of post-infectious irritable bowel syndrome

Compare¹,

Rocco²,

Coccoli³

et al. 2017

BMC Gastroenterol

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“…Several recent studies have suggested that patients with IBS, particularly those with a diarrhea-predominant subtype, have SIBO more often than controls using hydrogen breath tests and an upper gut aspirate culture [5][6][7]. Moreover, qualitative alteration of the gut flora, also known as dysbiosis, is being reported increasingly among patients with IBS [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…Patients with SIBO allocated to nor-P = NS). Although norfloxacin was more effective in reducing symptom score at 1 month among patients with SIBO than those without [15/80, 19% on culture, four on GHBT too; 6.5 (2-13) vs. 2 (0-10), P = 0.01; 8.5(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16) vs. 5 (0-12), P < 0.001] but not placebo, the scores were comparable at 6 months [3 (1-10) vs. 9(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19); P = NS]. At 1 month of follow-up, SIBO patients had symptom resolution that turned Rome III negative more…”

mentioning

confidence: 99%

A proof-of-concept study showing antibiotics to be more effective in irritable bowel syndrome with than without small-intestinal bacterial overgrowth

Ghoshal

Srivastava

Misra

et al. 2016

European Journal of Gastroenterology & Hepatology

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“…Though the pathophysiology of IBS remains largely enigmatic, evidence from recent studies does show that dysbiosis may contribute to development of symptoms, at least in a subset of patients 2,78. Though SIBO is a form of quantitative alteration of small bowel microbes, altered microbiota (dysbiosis) does not necessarily mean SIBO only.…”

Section: Pathogenesis Of Ibs Symptoms Among Patients With Sibomentioning

confidence: 99%

Small Intestinal Bacterial Overgrowth and Irritable Bowel Syndrome: A Bridge between Functional Organic Dichotomy

2017

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The pathogenesis of irritable bowel syndrome (IBS), once thought to be largely psychogenic in origin, is now understood to be multifactorial. One of the reasons for this paradigm shift is the realization that gut dysbiosis, including small intestinal bacterial overgrowth (SIBO), causes IBS symptoms. Between 4% and 78% of patients with IBS and 1% and 40% of controls have SIBO; such wide variations in prevalence might result from population differences, IBS diagnostic criteria, and, most importantly, methods to diagnose SIBO. Although quantitative jejunal aspirate culture is considered the gold standard for the diagnosis of SIBO, noninvasive hydrogen breath tests have been popular. Although the glucose hydrogen breath test is highly specific, its sensitivity is low; in contrast, the early-peak criteria in the lactulose hydrogen breath test are highly nonspecific. Female gender, older age, diarrhea-predominant IBS, bloating and flatulence, proton pump inhibitor and narcotic intake, and low hemoglobin are associated with SIBO among IBS patients. Several therapeutic trials targeting gut microbes using antibiotics and probiotics have further demonstrated that not all symptoms in patients with IBS originate in the brain but rather in the gut, providing support for the micro-organic basis of IBS. A recent proof-of-concept study showing the high frequency of symptom improvement in patients with IBS with SIBO further supports this hypothesis.

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Fecal Microbiota in Patients with Irritable Bowel Syndrome Compared with Healthy Controls Using Real-Time Polymerase Chain Reaction: An Evidence of Dysbiosis

Abstract: Fecal microbiota was different among IBS than HC, and different sub-types were associated with different microbiota. P. aeruginosa was more frequent and higher in number among IBS patients.

Cited by 114 publications

References 50 publications

Lactobacillus casei DG and its postbiotic reduce the inflammatory mucosal response: an ex-vivo organ culture model of post-infectious irritable bowel syndrome

Lactobacillus casei DG and its postbiotic reduce the inflammatory mucosal response: an ex-vivo organ culture model of post-infectious irritable bowel syndrome

A proof-of-concept study showing antibiotics to be more effective in irritable bowel syndrome with than without small-intestinal bacterial overgrowth

Small Intestinal Bacterial Overgrowth and Irritable Bowel Syndrome: A Bridge between Functional Organic Dichotomy

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