Lactobacillus casei DG and its postbiotic reduce the inflammatory mucosal response: an ex-vivo organ culture model of post-infectious irritable bowel syndrome

Compare, Debora; Rocco, Alba; Coccoli, Pietro; Angrisani, Debora; Sgamato, C.; Iovine, Barbara; Umberto, Salvatore; Nardone, Gerardo

doi:10.1186/s12876-017-0605-x

Cited by 99 publications

(72 citation statements)

References 29 publications

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“…These effects, as in the previous study, are associated with the reduction of intestinal epithelial permeability and the continuity of the intestinal barrier through postbiotic activity [60]. Comapare et al investigated the effect of the probiotic strain L. casei DG and its postbiotic on the modulation of the inflammatory response in patients with post-infectious IBS [61]. They proved that intestinal microbiota disorders are crucial in patients with irritable bowel syndrome, and the use of probiotics and postbiotics has a soothing effect on the course of the disease [61].…”

Section: Effects On the Risk Of Cardiovascular Eventssupporting

confidence: 52%

“…Comapare et al investigated the effect of the probiotic strain L. casei DG and its postbiotic on the modulation of the inflammatory response in patients with post-infectious IBS [61]. They proved that intestinal microbiota disorders are crucial in patients with irritable bowel syndrome, and the use of probiotics and postbiotics has a soothing effect on the course of the disease [61].…”

Section: Effects On the Risk Of Cardiovascular Eventsmentioning

confidence: 99%

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Influence of natural dairy probiotics on health

Wojciechowska-Alwin¹,

Sikora

Lipski

et al. 2019

J Pre Clin Clin Res.

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Introduction. Probiotics are live microorganisms which, administered in appropriate amounts, have a beneficial effect on human health. Food products that contain these microorganisms are known as natural probiotics. Probiotic food include the group of dairy products in which fermented milk products are majority. Objective. The aim of the study is to review current data and summarize knowledge on the effects of consumption of dairy probiotics on human health. The review also aims at discussing the potential of these health-supporting microorganisms as a prevention factor against civilization diseases. State of knowledge. Consumption of probiotic food, especially natural dairy probiotic food, may have a positive effect on health due to the presence of probiotic bacteria or by the presence of their metabolites (postbiotics) demonstrating bioactive effects. The intake of these products is associated with the improvement of parameters such as lipid profile, insulin sensitivity, cardiovascular risk parameters, or presents protective effect on bones. Studies show a correlation between the consumption of natural probiotics and reduction in duration of diarrhea or alleviation of the course of inflammatory bowel diseases. Conclusion. The influence of natural dairy probiotics consumption on the state of health has recently been broadly investigated. Regular consumption of these products has shown beneficial effect on gut microbiota and on a wide range of health parameters. However, further studies are necessary to draw a precise conclusion.

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Section: Effects On the Risk Of Cardiovascular Eventssupporting

confidence: 52%

Section: Effects On the Risk Of Cardiovascular Eventsmentioning

confidence: 99%

Influence of natural dairy probiotics on health

Wojciechowska-Alwin¹,

Sikora

Lipski

et al. 2019

J Pre Clin Clin Res.

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show abstract

“…In particular, postbiotics can act on immune cells protecting the gut tissue from immunopathology by increasing the secretion of anti-inflammatory cytokines such as IL-10. 160,161 Moreover, postbiotics potentiate epithelial tight junction structure by increasing the expression of tight junction proteins (zonula occludens, ZO-1) and intestinal mucin levels, thus favouring the restoration of gut barrier function. 162 In addition, postbiotics also behave as microbiota resilience-inducing factors.…”

Section: Postbioticsmentioning

confidence: 99%

The gut-liver axis in liver disease: Pathophysiological basis for therapy

Albillos

Gottardi

Rescigno

2020

Journal of Hepatology

1,162

881

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The gut-liver axis refers to the bidirectional relationship between the gut and its microbiota, and the liver, resulting from the integration of signals generated by dietary, genetic and environmental factors. This reciprocal interaction is established by the portal vein which enables transport of gut-derived products directly to the liver, and the liver feedback route of bile and antibody secretion to the intestine. The intestinal mucosal and vascular barrier is the functional and anatomical structure that serves as a playground for the interactions between the gut and the liver, limiting the systemic dissemination of microbes and toxins while allowing nutrients to access the circulation and to reach the liver. The control of microbial communities is critical to maintaining homeostasis of the gut-liver axis, and as part of this bidirectional communication the liver shapes intestinal microbial communities. Alcohol disrupts the gut-liver axis at multiple interconnected levels, including the gut microbiome, mucus barrier, epithelial barrier and at the level of antimicrobial peptide production, which increases microbial exposure and the proinflammatory environment of the liver. Growing evidence indicates the pathogenetic role of microbe-derived metabolites, such as trimethylamine, secondary bile acids, short-chain fatty acids and ethanol, in the pathogenesis of non-alcoholic fatty liver disease. Cirrhosis by itself is associated with profound alterations in gut microbiota and damage at the different levels of defence of the intestinal barrier, including the epithelial, vascular and immune barriers. The relevance of the severe disturbance of the intestinal barrier in cirrhosis has been linked to translocation of live bacteria, bacterial infections and disease progression. The identification of the elements of the gut-liver axis primarily damaged in each chronic liver disease offers possibilities for intervention. Beyond antibiotics, upcoming therapies centred on the gut include new generations of probiotics, bacterial metabolites (postbiotics), faecal microbial transplantation, and carbon nanoparticles. FXR-agonists target both the gut and the liver and are currently being tested in different liver diseases. Finally, synthetic biotic medicines, phages that target specific bacteria or therapies that create physical barriers between the gut and the liver offer new therapeutic approaches.

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“…L. paracasei DG has been demonstrated to possess the ability to modulate the intestinal microbial ecosystems of healthy adults ( Ferrario et al, 2014 ) and to influence host immune response ( Balzaretti et al, 2015 ; Cremon et al, 2017 ) via its unique polysaccharide capsule ( Balzaretti et al, 2017 ). L. paracasei DG has also been demonstrated to possess therapeutic potential for several dysfunctions and pathological conditions such as ulcerative colitis ( D’Incà et al, 2011 ), diverticular disease ( Tursi et al, 2013 ; Turco et al, 2017 ), small intestinal bacterial overgrowth ( Rosania et al, 2013 ), and irritable bowel syndrome ( Compare et al, 2017 ; Cremon et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Quantitative Recovery of Viable Lactobacillus paracasei CNCM I-1572 (L. casei DG®) After Gastrointestinal Passage in Healthy Adults

et al. 2018

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Probiotics are live microorganisms, and viability after transit through the gastrointestinal tract (GIT) is considered an inherent property of the health benefits of probiotics. The aim of the present study was to quantify the viable and total loads of Lactobacillus paracasei DG cells after passage through the GIT following the consumption of the probiotic product Enterolactis (L. casei DG®; L. paracasei CNCM I-1572; L. paracasei DG) from drinkable vials by healthy adults. We developed a novel method for discriminating and enumerating culturable L. paracasei DG cells based on the unique sticky, filamentous phenotype of this strain on MRS agar containing vancomycin and kanamycin. The identity of DG was also confirmed with strain-specific primers by colony PCR. This method was used for a recovery study of the DG strain to quantify viable cells in the fecal samples of 20 volunteers during a 1-week probiotic consumption period and a 1-week follow-up. We isolated L. paracasei DG from at least one fecal sample from all the volunteers. The highest concentration of viable DG cells [ranging from 3.6 to 6.7 log10 colony-forming unit (CFU) per gram of feces] in the feces was observed between 4 and 8 days from the beginning of Enterolactis intake and for up to 5 days after cessation of intake. As expected, the total DG count determined by real-time quantitative PCR (qPCR) was mostly higher than the viable DG cells recovered. Viable count experiments, carried out by combining ad hoc culture-based discriminative conditions and strain-specific molecular biological protocols, unambiguously demonstrated that L. paracasei DG can survive gastrointestinal transit in healthy adults when ingested as Enterolactis in drinkable vials containing no less than one billion CFU at the end of shelf life.

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Lactobacillus casei DG and its postbiotic reduce the inflammatory mucosal response: an ex-vivo organ culture model of post-infectious irritable bowel syndrome

Cited by 99 publications

References 29 publications

Influence of natural dairy probiotics on health

Influence of natural dairy probiotics on health

The gut-liver axis in liver disease: Pathophysiological basis for therapy

Quantitative Recovery of Viable Lactobacillus paracasei CNCM I-1572 (L. casei DG®) After Gastrointestinal Passage in Healthy Adults

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