Fecal Lactate and Ulcerative Colitis

Vernia, Piero; Caprilli, R.; Latella, Giovanni; Barbetti, Fabrizio; Magliocca, Fabio Massimo; Cittadini, M.

doi:10.1016/s0016-5085(88)80078-7

Cited by 226 publications

(181 citation statements)

References 20 publications

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“…Interestingly, the concentration of butyric acid was higher in the healthy subjects in the present study than in patients with ulcerative colitis (13.9 versus 11.1 mmol/g), whereas the concentration of lactic acid was lower (5.4 versus 15.9 mmol/g) (Hallert et al, 2003). Studies by others (Vernia et al, 1988) showed that faecal concentrations of butyric acid decreased, and concentrations of lactic acid increased with severity of ulcerative colitis, and that high colonic concentrations of lactic acid were associated with increased risk for diarrhoea and mucosal inflammation (Cummings, 1995). In the present study, the concentrations of lactic acid decreased during the intervention period with oat bran, whereas in our previous study in patients with ulcerative colitis, the faecal level of lactic acid was similar at entry of the study and after 4, 8 and 12 weeks of dietary supplementation with b-glucan-enriched oat bran (Hallert et al, 2003).…”

Section: Oat Bran Increases Faecal Cas U Nilsson Et Alcontrasting

confidence: 42%

Dietary supplementation with β-glucan enriched oat bran increases faecal concentration of carboxylic acids in healthy subjects

et al. 2007

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Background/Objective: Carboxylic acids (CAs), especially butyric acid, have been suggested to counteract colonic diseases, such as ulcerative colitis and colon cancer. Colonic formation of CAs can be influenced by the diet, but the concentrations and pattern formed need to be evaluated for different food products in humans. To elucidate how the colonic concentration of CAs in healthy subjects is influenced by dietary supplementation with oat bran, and whether the concentration varies over time and during consecutive days. Subjects/Methods: Twenty-five healthy subjects (age 2471.3) were recruited to the study. The subjects were given 40 g b-glucan enriched oat bran per day, corresponding to 20 g dietary fibre, in 4 slices of bread. CAs were analysed in faeces during three consecutive days after 0, 4, 8 and 12 weeks on this diet. Results: The concentration of acetic, propionic, butyric, isobutyric and isovaleric acid was higher (Po0.05-0.001) after 8 weeks on the oat bran diet as compared with values at entry, whereas that of lactic acid was lower (Po0.05). After 12 weeks, the concentrations of acetic, propionic and isobutyric acid were still higher and that of lactic acid lower. The variation between individuals was considerable, whereas in the same individuals there was little variation. Conclusions: Oat bran increased the faecal concentration of CAs after 8 weeks, indicating an increased concentration also in the distal colon. The concentration of all main acids increased, except for lactic acid, which decreased. Oat bran may therefore have a preventive potential adjunct to colonic diseases.

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Section: Oat Bran Increases Faecal Cas U Nilsson Et Alcontrasting

confidence: 42%

Dietary supplementation with β-glucan enriched oat bran increases faecal concentration of carboxylic acids in healthy subjects

et al. 2007

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“…Moreover, SCFAs regulate several leukocyte functions including production of cytokines (TNF-IL-2, IL-6 and IL-10), eicosanoids and chemokines [e.g., MCP-1 (macrophage chemoattractant protein-1) and CINC-2(cytokine induce neutrophil chemoattractant-2)]. Studies have reported that the concentration of these fatty acids in the gut and blood may predispose to or prevents pathological conditions such as IBD, cancer and diabetes [79][80][81]. SCFAs also modulate the production of prostaglandin E2 (PGE2) and have been shown to stimulate the in vitro production of this eicosanoid by human monocytes [82].…”

Section: Prebiotics and Immune Regulationmentioning

confidence: 99%

Induction of regulatory T cells: A role for probiotics and prebiotics to suppress autoimmunity

Dwivedi

Kumar

Laddha³

et al. 2016

Autoimmunity Reviews

119

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Section: Introductionmentioning

confidence: 99%

“…In particular, the composition of lactic acid, called non-volatile fatty acid (NVFA), increased in comparison with that of acetic, propionic, and butyric acids, called volatile fatty acid (VFA), in feces of ulcerative colitis patients. [7][8][9][10][11][12] Thus, the determination of fecal SCFAs is significant to examine homeostasis, characteristics, and metabolism of intestinal bacterial flora, and it would be useful for monitoring of and diagnostic strategy against diseases in the colon.The determination of SCFAs has been carried out by gas chromatography (GC) [13][14][15][16][17][18] and high-performance liquid chromatography with ultraviolet detection (HPLC-UV), fluorescent detection (HPLC-FL), or mass spectrometric detection (LC-MS). [19][20][21][22][23][24] The fecal analysis by GC required the complicate cleanup of the sample, and it was hard to detect NVFAs such as lactic acid.…”

mentioning

confidence: 99%

Determination of Short-chain Fatty Acids in Rat and Human Feces by High-Performance Liquid Chromatography with Electrochemical Detection

et al. 2009

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A simple method for determining short-chain fatty acids (SCFAs) in rat and human feces was developed using high-performance liquid chromatography with electrochemical detection (HPLC-ECD). A two-channel HPLC-ECD system was fabricated using an ion exclusion column and an electrochemical detector with a glassy carbon working electrode. Aqueous solutions of 0.1 mM HClO4 and of ethanol containing 2-methyl-1,4-naphthoquinone served as a mobile phase and a quinone solution, respectively. Peak areas for lactic, acetic, propionic, butyric, isovaleric, and valeric acids at a detection potential of -0.9 V vs. an Ag/AgCl electrode showed a linear relationship with the acid amount in the range 0.1 to 40 nmol. Standard acids at 4 nmol were determined ten times with relative standard deviations (RSD) of less than 2.0%. The analytical results of healthy human feces were measured within 35 min. RSD (n = 5) in all SCFAs were less than 2.7%, and recoveries of SCFAs were more than 92%. The present method was characterized by reproducibility with the simple and rapid procedure without derivatization of analytes, and it has the potential for clinical and biomedical applications. IntroductionThe bacteria in human colon obtain energy for growth by fermenting carbohydrates in the colonic lumen, mainly polysaccharides of plant cell walls (dietary "fiber") and some starch. The end-products include around 200 to 400 mmol day -1 of short-chain fatty acids (SCFAs) such as acetic, propionic, and butyric acids. Over 90% are absorbed and used by host, contributing 2 to 10% of daily energy requirements. 1,2 This is particularly important in premature babies with lactose malabsorption. 3 An extensive disease of the bowel, surgical bypass, prolonged antibiotic therapy, total parenteral nutrition, malabsorption, immaturity 4,5 and wide variation in dietary fiber intake may all disturb this important host/bacterial symbiosis. 1,2Since fecal SCFAs reflect colonic fermentation, 6 they are measured in studies to investigate and manipulate such disturbances. Moreover, fecal SCFA composition ratio of ulcerative colitis patients remarkably changes in comparison with that for a healthy human. In particular, the composition of lactic acid, called non-volatile fatty acid (NVFA), increased in comparison with that of acetic, propionic, and butyric acids, called volatile fatty acid (VFA), in feces of ulcerative colitis patients. [7][8][9][10][11][12] Thus, the determination of fecal SCFAs is significant to examine homeostasis, characteristics, and metabolism of intestinal bacterial flora, and it would be useful for monitoring of and diagnostic strategy against diseases in the colon.The determination of SCFAs has been carried out by gas chromatography (GC) [13][14][15][16][17][18] and high-performance liquid chromatography with ultraviolet detection (HPLC-UV), fluorescent detection (HPLC-FL), or mass spectrometric detection (LC-MS). [19][20][21][22][23][24] The fecal analysis by GC required the complicate cleanup of the sample, and it was hard to detect NVFAs ...

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Fecal Lactate and Ulcerative Colitis

Cited by 226 publications

References 20 publications

Dietary supplementation with β-glucan enriched oat bran increases faecal concentration of carboxylic acids in healthy subjects

Dietary supplementation with β-glucan enriched oat bran increases faecal concentration of carboxylic acids in healthy subjects

Induction of regulatory T cells: A role for probiotics and prebiotics to suppress autoimmunity

Determination of Short-chain Fatty Acids in Rat and Human Feces by High-Performance Liquid Chromatography with Electrochemical Detection

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