Fecal Carriage of Extended-Spectrum β-Lactamases in Healthy Humans, Poultry, and Wild Birds in León, Nicaragua—A Shared Pool of blaCTX-M Genes and Possible Interspecies Clonal Spread of Extended-Spectrum β-Lactamases-Producing Escherichia coli

Hasan, Badrul; Laurell, Karl; Rakib, Mufti Mahmud; Ahlstedt, Erik; Hernández, Jorge; Cáceres, Mercedes; Järhult, Josef D.

doi:10.1089/mdr.2015.0323

Cited by 31 publications

(24 citation statements)

References 43 publications

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“…The role of wildlife in the dissemination of resistant genes is supported by the recent isolation of ESBL‐producing bacteria carrying bla CTX‐M‐15 genes in wild birds of Nicaragua, Chile and Argentina, and raises public health concerns given the implications of these bacteria in both nosocomial and community‐acquired infections in humans (Guenther et al., ; Hasan et al., ; Liakopoulos et al., ). Our findings highlight the need to elucidate the role of bats and livestock in AMR bacterial transmission cycles and what risks these community sources may pose for zoonotic transmission to humans.…”

Section: Discussionmentioning

confidence: 99%

Extended‐spectrum beta‐lactamase‐producing Escherichia coli in common vampire bats Desmodus rotundus and livestock in Peru

Benavides

Shiva

Virhuez

et al. 2018

Zoonoses and Public Health

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Antibiotic resistance mediated by bacterial production of extended-spectrum beta-lactamase (ESBL) is a global threat to public health. ESBL resistance is most commonly hospital-acquired; however, infections acquired outside of hospital settings have raised concerns over the role of livestock and wildlife in the zoonotic spread of ESBL-producing bacteria. Only limited data are available on the circulation of ESBL-producing bacteria in animals. Here, we report ESBL-producing Escherichia coli in wild common vampire bats Desmodus rotundus and livestock near Lima, Peru. Molecular analyses revealed that most of this resistance resulted from the expression of bla genes carried by plasmids, which are disseminating worldwide in hospital settings and have also been observed in healthy children of Peru. Multilocus sequence typing showed a diverse pool of E. coli strains carrying this resistance that were not always host species-specific, suggesting sharing of strains between species or infection from a common source. This study shows widespread ESBL resistance in wild and domestic animals, supporting animal communities as a potential source of resistance. Future work is needed to elucidate the role of bats in the dissemination of antibiotic-resistant strains of public health importance and to understand the origin of the observed resistance.

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Section: Discussionmentioning

confidence: 99%

Extended‐spectrum beta‐lactamase‐producing Escherichia coli in common vampire bats Desmodus rotundus and livestock in Peru

Benavides

Shiva

Virhuez

et al. 2018

Zoonoses and Public Health

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“…Similar to camels, few studies reported the antimicrobial resistance profile in pigeons. In a low-income setting in Nicaragua, Hasan et al reported the existence of ESBLproducing E. coli in about 13% poultry pigeons compared to 8% in other domestic and wild birds, and 27% in healthy humans (Hasan et al, 2016). Interestingly, another study from South Africa addressed the risk of AMR E. coli spread due to pigeon fecal samples contamination of the roof-harvested rainwater (Chidamba & Korsten, 2015).…”

Section: Amr In Commensal E Coli From Pigeonsmentioning

confidence: 99%

Antimicrobial Resistance of Commensal Escherichia coli Isolated from Food Animals in Qatar

Alhababi

Eltai

Nasrallah

et al. 2020

Microbial Drug Resistance

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Background: The dissemination of antimicrobial resistance (AMR) bacteria is associated with the inappropriate use of antibiotics in both humans and animals. In particular, the use of antibiotics for prophylactic and growth promotion purposes in foodproducing animals, in addition to animal-group medication for prolonged times, have rendered many of the antibiotics ineffective, returning us to the old times when a simple infection could be deadly. Objectives: This study aims at evaluating the incidence of AMR in Qatar under the one health approach; i.e., human-animal-environmental interface.Specifically, we aim to characterize the phenotypic and genotypic AMR pattern of 18 clinically relevant antibiotics in major food-producing animals. Ultimately, we aim to compare our findings to the data released by the monitoring programs in Hamad Medical Corporation (HMC) along with previously published reports from Qatar. Methods: Animal fecal samples from camels, cattle, and pigeons (100 samples from each species) were collected from slaughterhouses and farms distributed in different locations in Qatar. One gram of fecal sample was vigorously homogenized with 3 ml sterile Phosphate Buffered Saline (PBS), the suspension was streaked directly onto chromogenic Tryptone Bile Xglucuronide agar for E. coli detection. Single typical E. coli colonies (blue-green) were randomly selected and streaked onto MacConkey agar plates and then recovered on iv nutrient agar plates for lactose fermentation testing by indole spot. The antibiotic susceptibility test was conducted using a Kirby-Bauer disk diffusion assay on Mueller-Hinton agar plates. Results: A total recovery rate of 88.7% (n=266) achieved from all samples. Overall, E. coli isolates had resistance to ten antibiotics in pigeon group, eight antibiotics in cattle group and only five antibiotics in camel group. Resistance to at least one antibiotic was recorded in 63 E. coli isolates (70.7%) from pigeons, 32 isolates (37.2%) from cattle and only in 19 isolates (20.8%) from camels. Multi-drug resistant (MDR) was highest in isolates from pigeons reaching 50% (n=44), followed by isolates from cattle (7%) and camels (2.2%). Highest resistance rate was observed against tetracycline, reaching a frequency of 64%, 27.9% and 15% of isolates from pigeons, cattle and camels, respectively (p <0.0001). Moreover, trimethoprim/sulfamethoxazole (an antibiotic used to treat a variety of bacterial infections) resistance was present in 22.2% (n=59) of all E. coli isolates, with the highest prevalence in pigeons (49.4%), followed by 11.6% in cattle and 5% in camels. Interestingly, one E. coli isolate from pigeon showed resistance to colistin, a drug of last resort in human medicine against gram-negative bacterial infection.Conclusions: We previously reported high multi-drug resistance of commensal E. coli in chickens, with significant resistance to colistin. We observed lower AMR profile in ruminants, presumably due to the different antibiotics used in the two animal industries.

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“…Illustrative map of Europe showing countries with description of E-ESBL in cattle and beta-lactamases type.2014;Dia et al, 2016;Fam et al, 2011;Hasan et al, 2016;Hern andez et al, 2012;Liao et al, 2017;Poirel et al, 2013;Ruiz et al, 2011;Sidjabat et al, 2010).…”

mentioning

confidence: 99%

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

Palmeira

Ferreira

2020

Heliyon

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Food producing animal is a global challenge in terms of antimicrobial resistance spread. Extended-spectrum betalactamase (ESBL)-producing Enterobacteriaceae are relevant opportunistic pathogens that may spread in many ecological niches of the One Health approach as human, animal and environment due to intestinal selection of antimicrobial resistant commensals in food production animals. Cattle production is a relevant ecological niche for selection of commensal bacteria with antimicrobial resistance from microbiota. Enterobacteriaceae show importance in terms of circulation of resistant-bacteria and antimicrobial resistance genes via food chain creating a resistance reservoir, setting up a threat for colonization of humans and consequent health risk. ESBL-producing Enterobacteriaceae are a threat in terms of human health responsible for life threatening outbreaks and silent enteric colonization of community populations namely the elder population. Food associated colonization is a risk difficult to handle and control. In a time of globalization of food trading, population intestinal colonization is a mirror of food production and in that sense this work aims to make a picture of ESBL-producing Enterobacteriaceae in animal production for food over the world in order to make some light in this reality of selection of resistant threats in food producing animal.

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Fecal Carriage of Extended-Spectrum β-Lactamases in Healthy Humans, Poultry, and Wild Birds in León, Nicaragua—A Shared Pool of bla_CTX-M Genes and Possible Interspecies Clonal Spread of Extended-Spectrum β-Lactamases-Producing Escherichia coli

Cited by 31 publications

References 43 publications

Extended‐spectrum beta‐lactamase‐producing Escherichia coli in common vampire bats Desmodus rotundus and livestock in Peru

Extended‐spectrum beta‐lactamase‐producing Escherichia coli in common vampire bats Desmodus rotundus and livestock in Peru

Antimicrobial Resistance of Commensal Escherichia coli Isolated from Food Animals in Qatar

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

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