Features of skin wound healing in rats with experimental chronic kidney disease

Pavlov, Sergey; Litvinova, O. B.; Babenko, Nataliia

doi:10.15421/022181

Cited by 3 publications

(1 citation statement)

References 32 publications

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“…Changes that occur during any of these phases may contribute to the development of a chronic wound and interfere with its healing. 9,10 The other extreme is excessive extracellular matrix formation with disrupted architecture, leading to organ fibrosis (such as hypertrophic scarring of the skin and keloid formation). 11 Skin wound healing involves metabolic reprogramming of glucose, lipids, amino acids and other nutrients that play a vital role in the proliferation, differentiation and migration of various cell types.…”

Section: Introductionmentioning

confidence: 99%

Features of metabolism in chronic wound remodelling

Pavlov,

Babenko,

Kumetchko

et al. 2024

Scripta Medica

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Background/Aim: The treatment of chronic wounds continues to be a pressing problem throughout the world. Healing occurs through some evolutionarily conserved biochemical pathways. The mechanisms of development of disorders of reparative regeneration are not fully understood. The work aimed to study the dynamics of changes in metabolic parameters during the healing of chronic wounds. Methods: Healthy Wistar rats were divided into two groups. The animals of the first group were intact. Chronic wounds were simulated for the animals of the second group. On days 7, 14 and 28 after wound creation, the animals were euthanised. Biochemical parameters such as glucose, total protein, albumin, cholesterol, urea, creatinine, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were assessed in the blood serum of animals. Results: It was found that the maximum decrease in glucose and total protein levels in the blood serum of animals in the experimental groups compared to intact animals was observed 2 weeks after surgery: the glucose concentration in rats was 1.7 times lower (p < 0.001). The level of albumin in the blood serum of experimental animals compared to intact animals was reduced by 1.5 times after 14 days (p < 0.001) and by 1.2 times after 28 days (p < 0.01). A week after surgery, the concentration of urea in the blood serum of experimental animals was 1.3 times higher (p < 0.01) than in intact rats and by day 28 after surgery, the urea level was 1.4 times higher (p < 0.001). The reduction in cholesterol and creatinine levels was not significant. An increase in AST, AST and ALP levels in the blood serum of experimental animals was shown. An increase in the blood serum of animals 7 days after surgery compared to the indicators of intact animals: ALP concentrations by 2.8 times (p < 0.001) and ALT concentrations by 1.4 times (p < 0.001) was established. The AST level significantly increased 14 days after surgery (p < 0.05). Conclusions: The study of metabolic parameters allows monitoring of the state of the body during the healing process of wounds to correct treatment tactics.

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Section: Introductionmentioning

confidence: 99%

Features of metabolism in chronic wound remodelling

Pavlov,

Babenko,

Kumetchko

et al. 2024

Scripta Medica

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The nature of changes in endocrine and immune factors at the initial stage of the formation of chronic wounds

Pavlov

Тамм

Komisova

et al. 2023

Modern Medical Technology

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Aim. The work aimed to study the nature of changes in endocrine signals (insulin and cortisol) and bioactive molecules that control inflammation and activation of process synthesis (interleukin-2 (IL-2), interleukin-8 (IL-8), vascular endothelial growth factor (VEGF), amino-terminal propeptide of type III procollagen (PIIINP)), at the initial stage of the formation of chronic wounds in the experiment. Materials and methods. The study was conducted in 2 groups of Wistar rats. The first group is represented by intact animals. Animals of the second group were modeled chronic wounds. On the 3rd day of the experiment, all animals were euthanized. The concentration of studied bioactive molecules was measured in blood serum by enzyme immunoassay. Selected areas of the wound were subjected to histological examination. Results. The levels of cortisol, insulin, VEGF, IL-2, and PIIINP in animals with chronic wounds at the stage of inflammation were significantly higher compared to intact animals. A tendency towards a decrease in the expression of IL-8 was shown. Relationships between the levels of bioactive molecules in animals of different groups were studied. According to the results of studies a correlation between the concentrations of IL-8 and cortisol in rats of the intact group was established. In the group of rats on the 3rd day after wound induction, a positive correlation was noted between the levels of IL-8 and IL-2. Wound defects healed under the scab. Microscopic analysis showed that the histoarchitectonics of tissues in the area of damage on the 3rd day corresponded to the classic manifestations of the process of healing wounds at this stage. The main part of the wound was filled with fibrin strands with a predominantly inflammatory cellular component. In some areas, signs of growth and development of young granulation tissue were noted. In the marginal sections, signs of re-epithelialization of the wound defect were observed. Conclusions. Even though in our study, the histoarchitectonics of tissues in the area of damage corresponded to the classical manifestations of the wound healing process at the stage of inflammation, the obtained changes and the relationship between endocrine and immune factors may be harbingers of the formation of the chronic wound in the future.

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Problems of healing chronic wounds

Babenko,

Litvinova,

Pavlov

et al. 2023

Modern Medical Technology

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Aim. The work aimed to analyze and highlight the problems of chronic wound healing and modern methods of stimulation of reparative processes according to literature sources. An analysis of the scientific literature was carried out, in which views on the problems of wound healing are presented, taking into account the factors affecting the formation and healing of chronic wounds, and modern methods that stimulate reparation processes. Identifying the problems of wound healing will allow a comprehensive approach to the assessment of the wound process, taking into account the factors affecting the healing of injuries. Modern scientists are actively discussing the problems of wound healing, researching factors influencing the reparative process, including the high prevalence of chronic wounds, diagnosis and treatment of wound infections, age of patients, and state of immunity. In the case of systemic diseases, such as diabetes, cardiovascular pathology, etc., there is a violation of microcirculation, and as a result, there are violations of the phasing of reparative processes at the cellular level. As a result, the alteration process does not end and the process becomes chronic. In case of a mechanical injury, when adaptation reserves are reduced, primarily of the neurohumoral system, a key role is played by the disruption of regulatory mechanisms at the cellular level. Wound treatment requires surgical treatment, stimulation of angiogenesis, and other reparative processes at the systemic and local levels. In recent years, various methods of stimulating reparative processes have been used in clinical practice to treat wounds: local administration of recombinant growth factors, use of platelet-rich plasma, biotechnological wound dressings, and artificial skin substitutes. One of the most promising areas of wound treatment is the development of methods for targeted activation of stem cells. However, there are still insufficient data for the practical application of this method. Clinically effective and safe methods are still being developed. Physical methods are promising for improving the healing of chronic wounds. However, the selection of optimal parameters for physical action continues to be an urgent task. Despite the positive results obtained using the methods described above, there is a need for additional experimental studies and randomized controlled trials. Conclusions. Chronic wounds develop as a result of severe systemic diseases, and major mechanical injuries against the background of a decrease in physiological reserves due to emotional and psychological stress, obesity, etc. Choosing the right method of influencing the reparative process, taking into account the leading mechanism of damage repair, becomes very important to ensure the best chance for optimal wound healing.

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Features of skin wound healing in rats with experimental chronic kidney disease

Cited by 3 publications

References 32 publications

Features of metabolism in chronic wound remodelling

Features of metabolism in chronic wound remodelling

The nature of changes in endocrine and immune factors at the initial stage of the formation of chronic wounds

Problems of healing chronic wounds

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