Features of Lipoperoxidation and Morphological Changes of the Lungs in Experimental Diabetes Mellitus

Zaiats, L. M.; Fedorchenko, Yuliya

doi:10.26724/2079-8334-2022-3-81-214-218

Cited by 3 publications

(3 citation statements)

References 11 publications

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“…The conducted studies showed that acute arginine-induced pancreatitis is accompanied by pronounced disorders of ultrastructural organization of A-II. Our data are consistent with the results of research by other scientists who studied the submicroscopic structure of the components of the respiratory part of the lungs in other pathological conditions [3,11,12].…”

Section: Resultssupporting

confidence: 93%

Submicroscopic changes of type II alveolocytes in the late development of experimental acute pancreatitis

Zaiats,

Pasichnyk,

Zukow

2024

J Educ Health Sport

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Background. Today, acute pancreatitis is one of the most common and severe visceral disorders in patients worldwide. The aim of the work was to study the dynamics of ultrastructural changes of type II alveolocytes in the long-term periods of development of experimental acute pancreatitis. Material and methods. The experiments were carried out on 54 white Wistar male rats weighing 180–220 g. The animals were divided into three groups: first — intact, second — control, third — experimental with a model of acute pancreatitis, which was reproduced by intraperitoneal administration of a 20% solution of L-arginine at a total dose of 5 g/kg at one-hour interval. The control group of animals was intraperitoneally injected with an equivalent dose of isotonic sodium chloride solution. All research were performed under sodium thiopental anesthesia at the rate of 60 mg/kg body weight. Lung tissue for electron microscopic examination was collected from the lower lobe of the left lung at 3–5 and 7 days. Pieces of lung tissue measuring 1×1×1 mm were fixed in a 2.5% glutaraldehyde solution, followed by additional fixation in a 1% osmium tetroxide solution. After dehydration, the material was poured into Epon-Araldite. Sections with a thickness of 20–50 nm obtained on “Tesla BS-490” ultramicrotome were studied in a PEM-125K electron microscope. Results. It was found that three days after the start of the study, along with dystrophic-destructive changes, type II alveolocytes in a state of increased functional activity were observed. Continuation of the experiment (5 days) leads to the progression of ultrastructural changes in type II alveolocytes are determined, which are most pronounced on the 7 day of the study. Conclusion. Acute experimental pancreatitis is accompanied by marked changes in the ultrastructural structure of type II alveolocytes. The nature and severity of structural changes of type II alveolocytes depends on the duration of the course of arginine-induced acute pancreatitis.

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Section: Resultssupporting

confidence: 93%

Submicroscopic changes of type II alveolocytes in the late development of experimental acute pancreatitis

Zaiats,

Pasichnyk,

Zukow

2024

J Educ Health Sport

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“…Electron microscopic studies have shown that in acute arginine-induced pancreatitis there are marked violations of the ultrastructural structure of the hemocapillaries of the alveolar wall. A number of other scientists point to changes of a similar nature under the action of exo-and endogenous factors [4,5,6].…”

Section: Resultsmentioning

confidence: 99%

Ultrastructural changes of hemocapillaries of the lungs in the late development of experimental acute pancreatitis

Zaiats,

Pasichnyk,

Zukow

2024

J Educ Health Sport

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Background. Today, acute pancreatitis is a common form of acute abdomen in the clinic, the incidence of which has been increasing in recent years. The aim of the work was to study the dynamics of ultrastructural changes of the hemocapillaries of the alveolar wall of the lungs in the late stages of experimental acute pancreatitis. Material and methods. The experiments were carried out on 54 white Wistar male rats weighing 180–220 g. The animals were divided into three groups: first — intact, second — control, third — experimental with a model of acute pancreatitis, which was reproduced by intraperitoneal administration of a 20% solution of L-arginine at a total dose of 5 g/kg at one-hour interval. The control group of animals was intraperitoneally injected with an equivalent dose of isotonic sodium chloride solution. All research were performed under sodium thiopental anesthesia at the rate of 60 mg/kg body weight. Lung tissue for electron microscopic examination was collected from the lower lobe of the left lung at 3–5 and 7 days. Pieces of lung tissue measuring 1×1×1 mm were fixed in a 2.5% glutaraldehyde solution, followed by additional fixation in a 1% osmium tetroxide solution. After dehydration, the material was poured into Epon-Araldite. Sections with a thickness of 20–50 nm obtained on “Tesla BS-490” ultramicrotome were studied in a PEM-125K electron microscope. Results. The ultrastructural analysis showed that already 3 days after the study, dystrophic-destructive changes, as well as adhesion and aggregation of leukocytes, were detected in the endothelial cells. As the study period increased (5–7 days), the intensity of changes in the hemocapillaries of the alveolar wall increased significantly. In the lumen erythrocyte sludge, thromboleukocyte adhesion and aggregation are determined in hemocapillaries. Conclusion. Acute experimental pancreatitis is accompanied by marked changes in the ultrastructural structure of hemocapillaries of the alveolar wall. The nature and severity of structural changes in the hemocapillaries of the alveolar wall depends on the duration of the course of arginine-induced acute pancreatitis.

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“…The molecular intricacies of diabetes mellitus involve a cascade of events that extend well beyond glucose dysregulation. Hyperglycemia, the hallmark feature of diabetes, triggers a milieu of biochemical alterations, including the formation of advanced glycation end-products (AGEs) and the activation of inflammatory pathways [4]. These molecular changes, in turn, contribute to a pro-inflammatory state and oxidative stress, with far-reaching consequences on various organ systems, including the respiratory apparatus.…”

Section: Introductionmentioning

confidence: 99%

Peculiarities of Respiratory Cellular Implications in Streptozotocin-Induced Diabetes: A Focus on Type I Alveolocytes

Fedorchenko,

Sahan,

Antymys

2023

Scientific and practical journal

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Introduction. The escalating global prevalence of diabetes mellitus has ignited concerns, given its multifaceted repercussions that extend beyond conventional metabolic considerations. Recent scientific inquiries have firmly established a profound association between diabetes mellitus and abnormalities within the respiratory system, unveiling a nuanced interplay that transcends mere glycemic control. This study delves into the intricate and far-reaching influence of diabetes on the respiratory system, recognizing it as a vulnerable target organ susceptible to systemic repercussions. Compelling epidemiological evidence underscores a heightened prevalence of respiratory complications among individuals grappling with diabetes. This observation prompts a meticulous exploration of the pathophysiological underpinnings of this intricate relationship, spanning from molecular intricacies to clinical manifestations. The aim was to deepen our understanding of the impact of diabetes mellitus on the respiratory system by investigating the pathological alterations in type I alveolocytes within an experimental model of diabetes mellitus. Methods. In this experimental model we used 88 male Wistar rats (170-210 g). The rats were divided into three groups: Group 1 (n=10) consisted of intact rats; Group 2 (n=40) served as the control group, and Group 3 (n=38) constituted the experimental group where diabetes was induced by intraperitoneal administration of streptozotocin (Sigma, USA) diluted in 0.1 M citrate buffer with pH 4.5 at a dosage of 60 mg/kg of body weight. The control group received an equivalent volume of 0.1 M citrate buffer solution with a pH of 4.5 via intraperitoneal injection. All procedures were performed under sodium thiopental anesthesia at a dose of 60 mg/kg of body weight. Tissue samples were collected at intervals of 14, 28, 42, and 70 days post streptozotocin injection. For electron microscopy analysis, fragments of lung tissue were immersed in a 2.5% glutaraldehyde solution for fixation, followed by fixation in a 1% osmium tetroxide solution. After dehydration, the specimens were embedded in Epon Araldite. Sections, obtained through a "Tesla VS-490" ultramicrotome, underwent examination using a "PEM-125K" electron microscope. Results. Throughout the experiment, discernible changes in type I alveolocytes were observed. At 14 days, nuclei exhibited rounding or oval shape with uniform chromatin distribution. Mitochondria displayed small size, and the Golgi apparatus (GA) and granulated endoplasmic reticulum (GER) showed no significant alterations. By 28 days, nuclei adopted an oval shape, chromatin localized peripherally, and mitochondria exhibited diverse morphologies. Increased micropinocytotic vesicles indicated heightened cellular activity. At 42 days, hyperhydration became pronounced, nuclei displayed lower electron density, and cellular components showed advanced changes. By 70 days, dystrophic-destructive changes included low electron density nuclei, disorganized mitochondria, and fragmented GA and GER. Conclusion. This comprehensive ultrastructural analysis unveils the progressive impact of diabetes on type I alveolocytes, elucidating unique facets of pulmonary alterations over time. This study contributes to a growing body of knowledge, shedding light on the dynamic nature of pulmonary changes in diabetes mellitus, ultimately urging further exploration for a holistic understanding of its implications on respiratory health.

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Features of Lipoperoxidation and Morphological Changes of the Lungs in Experimental Diabetes Mellitus

Cited by 3 publications

References 11 publications

Submicroscopic changes of type II alveolocytes in the late development of experimental acute pancreatitis

Submicroscopic changes of type II alveolocytes in the late development of experimental acute pancreatitis

Ultrastructural changes of hemocapillaries of the lungs in the late development of experimental acute pancreatitis

Peculiarities of Respiratory Cellular Implications in Streptozotocin-Induced Diabetes: A Focus on Type I Alveolocytes

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