Feather hydrolysate from Streptomyces sampsonii GS 1322: A potential low cost soil amendment

Jain, Richa; Jain, Aakanchha; Rawat, Neha; Meera, Nair; Gumashta, Raghvendra

doi:10.1016/j.jbiosc.2015.11.003

Cited by 21 publications

(13 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thereinto, Streptomyces sampsonii is widely distributed in nature, having been isolated from Maytenus aquifolia Mart. [16], soil [17][18][19][20][21][22], marine sediment [23,24], and medicinal plants [25]. In particular, numerous isolates have been obtained from soil, such as garden soil [18], alpine soil from the Himalayan glacier region [19], fertile soil in Korea [26], and rhizosphere soil of healthy poplar trees [20].…”

Section: Introductionmentioning

confidence: 99%

Cloning and Expression of the Chitinase Encoded by ChiKJ406136 from Streptomyces Sampsonii (Millard & Burr) Waksman KJ40 and Its Antifungal Effect

Zhang

Zhu

et al. 2018

Forests

View full text Add to dashboard Cite

The present study demonstrated that the chitinase gene ChiKJ406136 of Streptomyces sampsonii (Millard & Burr) Waksman KJ40 could be cloned using a PCR protocol and expressed in Escherichia coli (Migula) Castellani & Chalmers BL21 (DE3), and the recombinant protein had antifungal effect on four forest pathogens (Cylindrocladium scoparium Morgan, Cryphonectria parasitica (Murrill) Barr, Neofusicoccum parvum Crous, and Fusarium oxysporum Schl.) and also had the biological control effects on Eucalyptus robusta Smith leaf blight, Castanea mollissima BL. blight, Juglans regia L. blight and J. regia root rot. The results showed that ChiKJ406136 was efficiently expressed and a 48 kilodalton (kDa) recombinant protein was obtained. No significant change in protein production was observed in the presence of different concentrations of IPTG (isopropyl-b-D-thio-galactoside). The purified protein yield was greatest in the 150 mmol/L imidazole elution fraction, and the chitinase activities of the crude protein and purified protein solutions were 0.045 and 0.033 U/mL, respectively. The antifungal effects indicated that mycelial cells of the four fungi were disrupted, and the control effects of the chitinase on four forest diseases showed significant differences among the undiluted 10-and 20-fold dilutions and the control. The undiluted solution exhibited best effect. The results of this study provide a foundation for the use of S. sampsonii as a biocontrol agent and provides a new source for the chitinase gene, providing a theoretical basis for its application. & Grigoraki, Trichophyton sp. (Castell.) Sabour. [18] and Rhizoctonia violacea (Tul.) Pat. [20]. Previous studies demonstrated that the bioactive compounds of S. sampsonii have important applications in various fields [3]. For example, crude extracts showed antitumor activity against glioblastoma multiforme (GBM) cells, inhibiting cell growth by 70.04% [25], and the supernatant of a S. sampsonii culture showed biological activity against the root-knot nematode [26]. In the purified components, soil isolates of S. sampsonii can produce heptaene polyene antibiotics [18,27], In addition, S. sampsonii has been shown to produce hydrolytic enzymes, such as amylase, chitinase, protease, and lipase [19]. Studies at the molecular level have focused on strain identification and the phylogenesis of related species [28][29][30][31][32][33][34][35]. The complete genome sequence of Streptomyces sampsonii KJ40 was recently described by our lab [36], resulting in the discovery of a large number of gene encoding chitinases and enzymes involved in secondary metabolite production. However, little is known regarding the metabolic pathways and genetic regulation in this strain, limiting its practical application.Chitin, a linear polymer of β-1,4-glucosidicosamine (GlcNAC), is the second most abundant polysaccharide in nature. Chitin can be degraded by chitinolytic enzymes, that is chitinase. Chitinases (EC 3.2.1.14) are widely present in a great variety of organisms, including insects, fungi,...

show abstract

Section: Introductionmentioning

confidence: 99%

Cloning and Expression of the Chitinase Encoded by ChiKJ406136 from Streptomyces Sampsonii (Millard & Burr) Waksman KJ40 and Its Antifungal Effect

Zhang

Zhu

et al. 2018

Forests

View full text Add to dashboard Cite

show abstract

“…Means followed by the same letter, for each parameter, do not differ by Tukey's test (p > 0.05) also indirectly enhance plant growth through microorganism-mediated soil processes (Colla et al 2015). Ammonifying, nitrifying, N 2 fixing, and P-solubilizing bacteria are among the beneficial microbial populations stimulated by FHs in soils (Paul et al 2013;Bose et al 2014;Jain et al 2016).…”

Section: Resultsmentioning

confidence: 98%

“…FH obtained with Bacillus pumilus KHS-1 was equivalent to a reference fertilizer in terms of growth, root DW and shoot DW of carrot and Chinese cabbage (Kim et al 2005). Also, feather hydrolysates obtained through microbial conversion displayed promising results as fertilizers for wheat (Jain et al 2016), rice (Tamreihao et al 2017), ryegrass (Gousterova et al 2011), chickpea (Paul et al 2013), and banana plants (Gurav and Jadhav 2013).…”

Section: Resultsmentioning

confidence: 99%

Feather hydrolysate as a promising nitrogen-rich fertilizer for greenhouse lettuce cultivation

Sobucki

Ramos

Gubiani

et al. 2019

Int J Recycl Org Waste Agricult

View full text Add to dashboard Cite

Purpose Poultry agro-industry generates huge amounts of solid wastes, such as poultry litter (PL) and feathers. Feathers, possessing a substantial amount of organic nitrogen, are mainly converted into feather meal (FM) through the hydrothermal process; however, microbial conversion into feather hydrolysates (FH) is increasingly being focused on. Since waste management strategies include the use of organic wastes as soil fertilizer, PL, FM, and FH were evaluated as fertilizers for lettuce in comparison to urea. Methods Lettuce seedlings were transplanted to soil pots containing FH, obtained through feathers' bioconversion by Bacillus sp. CL18, FM, PL, urea, and unfertilized controls. Partial chemical characterization of FH, FP, and PL was performed. Fertilization treatments were applied to reach 180 kg N/ha. Growth, dry biomass, and chlorophyll contents of lettuce were evaluated to indicate fertilizer performance. Results After 60 days of greenhouse lettuce cultivation, leaves per plant were superior after FH treatment. Lettuce fertilized with urea and FH presented higher and similar foliar areas. FH treatment resulted in increased dry weight (DW) of leaves, which then decreased in the order urea > FM > MM ~ controls > PL. Dry weight of leaves and roots was 33% and 64% higher with FH than with urea. Leaf total chlorophyll content was higher with FH, but similar to urea and FM. Lettuce growth was negatively affected by PL when compared to controls. Conclusion FH, obtained from microbial processing of abundant and inexpensive feathers, could be a candidate N-rich fertilizer for lettuce cultivation.

show abstract

“…Streptomyces sampsonii and its bioactivity is less reported 22–24 . In addition, the antifouling activity of Streptomyces sampsonii is understudied.…”

Section: Discussionmentioning

confidence: 99%

Isolation, characterization and identification of antibiofouling metabolite from mangrove derived Streptomyces sampsonii PM33

Venugopal

Radhakrishnan

Shanmugasundaram

et al. 2019

Sci Rep

View full text Add to dashboard Cite

In this study, we report the production, bioassay guided isolation and identification of antibiofouling metabolite from mangrove derived actinobacterium, Streptomyces sampsonii (PM33). The actinobacterial strain PM33 yields maximum amount of antifouling compounds through agar surface fermentation. In optimization, carbohydrates such as glucose, fructose and xylose, are suitable for maximum production of the active compound. In addition, other compounds such as malt extract, glutamine, and sodium chloride concentrations (2.5, 5 and 7.5%) and parameters such as pH 7.0 and temperature range 30 °C to 40 °C enhanced the production of antifouling metabolite. The antifouling metabolite was extracted in ethyl acetate. TLC and bioautography was used to separate and detect the antifouling metabolite present in the crude extract. The physico chemical features revealed that the antifouling metabolite PM33 – B as taxifolin (C15H12O7). The purified taxifolin was found to be active against biofouling bacteria, algal spore germination and mollusc foot adherence, respectively. Toxicity nature of taxifolin was also determined by adopting zebrafish embryos. The taxifolin isolated from mangrove-derived Streptomyces sampsonii PM33 is a promising candidate for the development of eco-friendly antifouling preparation.

show abstract

Feather hydrolysate from Streptomyces sampsonii GS 1322: A potential low cost soil amendment

Cited by 21 publications

References 28 publications

Cloning and Expression of the Chitinase Encoded by ChiKJ406136 from Streptomyces Sampsonii (Millard & Burr) Waksman KJ40 and Its Antifungal Effect

Cloning and Expression of the Chitinase Encoded by ChiKJ406136 from Streptomyces Sampsonii (Millard & Burr) Waksman KJ40 and Its Antifungal Effect

Feather hydrolysate as a promising nitrogen-rich fertilizer for greenhouse lettuce cultivation

Isolation, characterization and identification of antibiofouling metabolite from mangrove derived Streptomyces sampsonii PM33

Contact Info

Product

Resources

About