Feasibility study of a salivary occult blood test to correlate with periodontal measures as indicators of periodontal inflammation in a population of pregnant women

Reed, Susan G.; Manz, Michael C.; Snipe, Shannon M.; Ohshima, Mitsuhiro; Wagner, Carol L.

doi:10.2334/josnusd.57.55

Cited by 11 publications

(11 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, various substances in GCF have been suggested as useful biomarkers for detecting periodontitis such as: host-derived enzymes and their inhibitors, inflammatory mediators and host-response modifiers, and byproducts of tissue breakdown [31]. However, most of them have not seen clinical application except for a few systems such as aspartate aminotransferase enzyme kit (Periogard) [32], neutral proteases detection kit (Periocheck) [33], and occult blood test paper (Perioscreen) [10][11][12].…”

Section: Discussionmentioning

confidence: 99%

“…With a positive specimen, the conjugate bound to the anti-human hemoglobin antibody as a captured regent on the test band area and produced a red-violet colored band (positive line). Absence of a colored test band indicated a negative result [10][11][12]. After 5 min of reaction, visual evalu-ation of the test paper was distinguished as positive (++++, +++, ++, +) or negative (±, −) by 3 blinded examiners (AA, SK, and SM).…”

Section: Hb Detection By Immunochromatographic Paper Test Stripmentioning

confidence: 99%

“…Previously, Ohshima et al [10][11][12] invented a salivary occult blood test using an immunochromatographic paper strip for detecting periodontal disease. The salivary occult blood test can efficiently detect oral hemoglobin (Hb), in saliva or oral rinses using water, using a special paper strip coated with anti-human hemoglobin monoclonal antibody.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Detection of hepatocyte growth factor in oral rinses using water for possible periodontal diagnosis

et al. 2020

Self Cite

View full text Add to dashboard Cite

The aim of this study is to analyze the relationship between Hepatocyte Growth Factor (HGF) levels in oral rinses using water and clinical parameters of periodontitis; and furthermore, to evaluate the potential of a prototype HGF immunochromatographic paper test strip (HGF-TS) for screening of periodontitis, in comparison with a commercially-available occult blood (hemoglobin) test strip (Hb-TS). Clinical periodontal parameters were recorded, and oral rinses were collected, from 125 subjects. Then, the presence of HGF, and hemoglobin (Hb), in each sample was detected using a prototype HGF-TS and an Hb-TS. In addition, the concentrations of HGF and Hb were also determined in each sample is necessary HGF concentrations in oral rinses showed significant correlations with clinical parameters of periodontitis. The positive rate and read value on HGF-TS showed significantly high values in cases of severe periodontitis compared to healthy subjects. Hb-TS showed generally higher positive rates than HGF-TS; however, it showed false positive results in healthy subjects. The concentration of HGF in oral rinses showed close association with the severity of periodontitis, suggesting that the prototype HGF-TS has potential for use in the diagnosis of periodontitis, although further refinement of the test strip is required to increase the sensitivity. Keywords; hepatocyte growth factor, immunochromatgraphic paper, mass-screening test, occult blood test, periodontitis Materials and Methods Subjects and clinical evaluation of periodontal status A total of 125 subjects (60 male and 65 female) participated in this study. The subjects with periodontal disease were patients of the Department of Periodontics, Dental Hospital of Tokyo Medical and Dental University (TMDU). The healthy subjects were volunteers consisting of dentists in the Department of Periodontology, TMDU, as well as dentists and hygienists from private dental offices. Written informed consent for participation was obtained from all individuals. This study was approved by the Ethics Committee of the Faculty of Dentistry, TMDU (#1209), and the study was conducted in accordance with the Helsinki Declaration of 1975, as revised in 2013, and the ethical guidelines for epidemiology research of 2002, as revised in 2008 (Ministry of Health, Labor and Welfare, Japan). Subjects were selected according to the following criteria: absence of systemic diseases, no systemic antibiotics taken in preceding 3 months, and no pregnancy or lactation. Participants underwent a full-mouth periodontal examination including probing pocket depth (PD) and bleeding on probing (BOP) at all six sites of each tooth, as well as tooth mobility. Examinations were conducted by each patient's dentist. Radiographic examination (bisecting angle technique) was performed for some of the periodontitis patients and healthy subjects, and bone crest level (BCL) was determined. BCL was defined as the vertical distance (mm) from cementoenamel junction (CEJ) to the most crestal point of marginal bone [19-21], and w...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Hb Detection By Immunochromatographic Paper Test Stripmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Detection of hepatocyte growth factor in oral rinses using water for possible periodontal diagnosis

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, in terms of predicting the prevalence of periodontal disease, the cut-off points of 1.0 and 2.0 μg/mL need to be supported by more evidence before adoption. Furthermore, various other techniques have been reported to be more sensitive in the detection of haemoglobin in saliva, 21,45 including a commercially available kit that has been developed as a screening tool for the determination of periodontal status that correlates well to bleeding on probing. 45 Although our results and that of others [20][21][22][23]45 suggest that salivary haemoglobin testing may be appropriate for periodontal disease screening, further research is necessary in this area to determine appropriate cut-off points and protocols.…”

Section: Discussionmentioning

confidence: 99%

Relationship between salivary haemoglobin and number of remaining teeth in older Koreans

2017

View full text Add to dashboard Cite

Objective: We investigated the relationship between saliva and dental biofilm characteristics, oral hygiene behaviours and the number of remaining teeth in a sample of older Koreans. Materials and methods:This cross-sectional study included 133 participants, aged 65 years and older, recruited from senior welfare facilities in Incheon, South Korea.The outcome variable was the number of remaining teeth, and its associations with the independent variables of participants' general characteristics, salivary haemoglobin level, dental biofilm acidogenicity, salivary flow rate and oral hygiene behaviours were assessed. For statistical analyses, chi-squared test and step-wise multiple linear regression were used. Results:The multiple linear regression model, which included all related factors identified in the bivariate analyses, showed that older adults who had high salivary haemoglobin levels (P < .05), brushed their teeth using the horizontal scrub method (P< .001), and did not use an interproximal cleaning device (P < .01) were more likely to have fewer remaining teeth. However, dental biofilm acidogenicity was not associated with the number of remaining teeth. Conclusion:The number of remaining teeth was associated with salivary haemoglobin level, appropriate toothbrushing technique and interdental cleaning. These findings suggest that the monitoring of salivary haemoglobin may contribute to the prevention of tooth loss caused by periodontal disease. K E Y W O R D Sgeriatric dentistry, haemoglobin, oral hygiene, saliva, tooth loss

show abstract

“…Additionally, we investigated hemoglobin positivity using a salivary occult blood test (SOBT) to screen for periodontal disease. An SOBT using monoclonal antibody against human hemoglobin has been used in investigating periodontal health, and it may be useful as a screening method for periodontal disease [7, 8]. An SOBT using anti-human hemoglobin monoclonal antibody has been used to reliably assess periodontal inflammation in pregnant women [7].…”

Section: Introductionmentioning

confidence: 99%

Relationship of Salivary Occult Blood With General and Oral Health Status in Employees of a Japanese Department Store

et al. 2019

View full text Add to dashboard Cite

Background The Japanese Ministry of Health started screening for periodontal disease as part of senior health services in 1995. However, only a small number of workplaces conduct regular dental checkups in Japan. Therefore, the prevalence of periodontal disease and other oral health conditions has not been fully determined in workers in Japan. This study aimed to clarify the usefulness of a salivary occult blood test (SOBT) to assess periodontal disease, and to investigate the association of salivary occult blood with general and oral health in Japanese employees. Methods A cross-sectional study was conducted among department store workers in Hiroshima city. Subjects were 449 workers who received regular health checkups including dental examinations in 2018. An SOBT using monoclonal antibody against human hemoglobin was performed. Periodontal status was evaluated using the Community Periodontal Index (CPI). We investigated the association of salivary occult blood with general and oral health in 338 employees (85 men, 253 women; mean age 41.4 years, range 19 - 69 years). Results Univariate analysis revealed a significant relationship between salivary occult blood and sex, body mass index (BMI), diabetes, CPI, daily brushing frequency, and number of untreated teeth (P = 0.034, P = 0.003, P = 0.022, P = 0.007, P = 0.004, and P = 0.015, respectively). Furthermore, BMI, diabetes, CPI, and brushing frequency were significantly associated with salivary occult blood in binomial logistic regression analysis (odds ratio 1.09, P = 0.014; odds ratio 9.38, P = 0.047; odds ratio 1.31, P = 0.004; and odds ratio 0.70, P = 0.045, respectively). These results suggest that positivity in the SOBT is importantly associated with periodontal disease and diabetes. Interestingly, subjects aged ≥ 35 years with metabolic syndrome exhibited a significantly higher positive rate of salivary occult blood than those without metabolic syndrome (P = 0.01). Conclusions The SOBT was a reliable screening method for periodontal disease, and positivity in the test was related to diabetes in Japanese employees. Further examinations are required to clarify the association of salivary occult blood with other systemic diseases.

show abstract

Feasibility study of a salivary occult blood test to correlate with periodontal measures as indicators of periodontal inflammation in a population of pregnant women

Cited by 11 publications

References 17 publications

Detection of hepatocyte growth factor in oral rinses using water for possible periodontal diagnosis

Detection of hepatocyte growth factor in oral rinses using water for possible periodontal diagnosis

Relationship between salivary haemoglobin and number of remaining teeth in older Koreans

Relationship of Salivary Occult Blood With General and Oral Health Status in Employees of a Japanese Department Store

Contact Info

Product

Resources

About