Feasibility study for the development of a certified reference material of nitrofuran metabolites in chicken breast muscle from incurred samples

Violante, Fernando Gustavo Marques; Rosas, Carla de Oliveira; Guimarães, Evelyn de Freitas; Vital, Hélio de Carvalho; Zúniga, Nathália Oliveira Cavalcanti; Neto, Francisco Radler de Aquino

doi:10.1016/j.measurement.2018.07.027

Cited by 16 publications

(4 citation statements)

References 6 publications

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“… 2 , 3 These drugs metabolize rapidly within a few hours after ingestion, and the residual metabolites could remain in the body for weeks, possibly even months, as protein-bound compounds. 4 − 6 These NF metabolite compounds have been proven to pose potential risk to human health because of their carcinogenic, teratogenic, and mutagenic potency. 5 , 7 Based on food safety needs, the use of NF drugs was banned by the European Union during the cultivation of animal products.…”

Section: Introductionmentioning

confidence: 99%

“…Many analytical methods have been developed for the detection of NF metabolites in many matrices, such as shrimp, 26 animal feed, 27 meat, 4 seafood, 28 milk, 29 honey, 30 , 31 and so on. 13 , 32 These analytical methods include liquid chromatography-tandem mass spectrometry (LC-MS), 33 high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), 30 , 34 ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), 35 , 36 enzyme-linked immunosorbent assay (ELISA), 37 etc.…”

Section: Introductionmentioning

confidence: 99%

“…Nitrofuran (NF), mainly including furazolidone (FZD), nitrofurazone (NFZ), nitrofurantoin (NFT), and furaltadone (FTD), are a class of synthetic broad-spectrum antibacterial drugs with a 5-nitro structure that were commonly used in veterinary drugs for the treatment of protozoan and gastrointestinal infections. , These drugs metabolize rapidly within a few hours after ingestion, and the residual metabolites could remain in the body for weeks, possibly even months, as protein-bound compounds. − These NF metabolite compounds have been proven to pose potential risk to human health because of their carcinogenic, teratogenic, and mutagenic potency. , Based on food safety needs, the use of NF drugs was banned by the European Union during the cultivation of animal products. , Since then, many countries have followed the suit, such as the United States, China, and Japan. − However, because of the low price and significant efficacy, NF drugs are still allowed or illegally used as veterinary drugs in some developing countries. , At present, the minimum performance limit (MRPL) of these residual compounds in meat products stipulated by the EU is 1.0 μg/kg. , In this context, it is essential to develop a fast and sensitive detection method for NF metabolite compounds for solving food safety problems.…”

Section: Introductionmentioning

confidence: 99%

“…Many analytical methods have been developed for the detection of NF metabolites in many matrices, such as shrimp, animal feed, meat, seafood, milk, honey, , and so on. , These analytical methods include liquid chromatography-tandem mass spectrometry (LC-MS), high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), , ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), , enzyme-linked immunosorbent assay (ELISA), etc. , Although many methods have been developed, they still cannot meet the detection requirements because of the extraordinarily low detection limit requirements of NF metabolites (MRPL = 1.0 μg/kg) . HPLC-MS/MS has been widely used because it has high sensitivity and accuracy so it can be used for the detection of NF metabolites .…”

Section: Introductionmentioning

confidence: 99%

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Determination of Nitrofuran Metabolites in Fish by Ultraperformance Liquid Chromatography-Photodiode Array Detection with Thermostatic Ultrasound-Assisted Derivatization

et al. 2020

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Nitrofuran (NF) is a class of broad-spectrum antibiotics that are used illegally in animal feeding. NF and its metabolites have proven to pose potential risk to human health. To address the current analytical needs to quantify low levels of NF metabolites in animal foods, a sensitive method was developed for simultaneous detection of four NF metabolites in fish products by an ultraperformance liquid chromatography-diode array detector (UPLC-DAD). With 2-nitrobenzaldehyde (2-NBA) as the derivatizing reagent, the metabolites were hydrolyzed and derivatized under the assistance of thermostatic ultrasound. Compared with the current detection methods, the time of the derivatization reaction has been shortened from 16 to 2 h. The relative coefficient of four NF metabolite derivatives reached more than 0.998, with excellent linear relationship. The limits of detection (LODs) and limits of quantification (LOQs) of six repeated determinations reached 0.25–0.33 and 0.80–1.10 μg/kg, respectively. For all four NF metabolites, the limit of detection of the method was below the minimum required performance limit (MRPL) of 1.0 μg/kg, which makes it compatible with the EU requirements. The recoveries ranging from 89.8 to 101.9% with relative standard deviation below 6.5% were obtained for all of the NF metabolites. What’s more, this method was successfully applied for the determination of four NF metabolites in the fish products. As a promising approach, this method could also be extended for the quantitation of NF metabolites in aquaculture and poultry products.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Determination of Nitrofuran Metabolites in Fish by Ultraperformance Liquid Chromatography-Photodiode Array Detection with Thermostatic Ultrasound-Assisted Derivatization

et al. 2020

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New matrix certified reference material for accurate measurement of ciprofloxacin residue in egg

et al. 2020

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Production and certification of BOTS-1: bovine muscle–certified reference material for incurred veterinary drug residues

et al. 2023

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A freeze-dried bovine muscle–certified reference material (CRM), known as BOTS-1 (DOI: https://doi.org/10.4224/crm.2018.bots-1), containing incurred residues of commonly used veterinary drugs was produced and certified for the mass fraction of eight veterinary drug residues. Value assignment was carried out using liquid chromatography tandem mass spectrometry (LC–MS/MS) methods in conjunction with isotope dilution and standard addition approaches involving stable isotope internal standards. Data from the National Research Council of Canada (NRC), Canadian Food Inspection Agency (CFIA), United States Department of Agriculture (USDA), and the Federal Office of Consumer Protection and Food Safety in Germany (BVL) were used for value assignment. Results for two drug residues were also obtained through an international inter-laboratory comparison CCQM-K141/P178 organized under the auspices of the International Bureau of Weights and Measures (BIPM). Quantitative NMR (1H-qNMR) was used to characterize primary standards of all veterinary drugs certified. The certified mass fractions of the veterinary drug residues were 490 ± 100 µg/kg for chlorpromazine, 44 ± 4.4 µg/kg for ciprofloxacin, 3.3 ± 1.4 µg/kg for clenbuterol, 9.5 ± 0.8 µg/kg for dexamethasone, 57 ± 4.8 µg/kg for enrofloxacin, 3.0 ± 0.4 µg/kg for meloxicam, 12.4 ± 1.2 µg/kg for ractopamine, and 2290 ± 120 µg/kg for sulfadiazine with expanded uncertainties quoted (95% confidence) which include the effects due to between-bottle inhomogeneity, instability during long-term storage and transportation, and characterization.

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Feasibility study for the development of a certified reference material of nitrofuran metabolites in chicken breast muscle from incurred samples

Cited by 16 publications

References 6 publications

Determination of Nitrofuran Metabolites in Fish by Ultraperformance Liquid Chromatography-Photodiode Array Detection with Thermostatic Ultrasound-Assisted Derivatization

Determination of Nitrofuran Metabolites in Fish by Ultraperformance Liquid Chromatography-Photodiode Array Detection with Thermostatic Ultrasound-Assisted Derivatization

New matrix certified reference material for accurate measurement of ciprofloxacin residue in egg

Production and certification of BOTS-1: bovine muscle–certified reference material for incurred veterinary drug residues

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