Feasibility of desorption atmospheric pressure photoionization and desorption electrospray ionization mass spectrometry to monitor urinary steroid metabolites during pregnancy

Vaikkinen, Anu; Rejšek, Jan; Vrkoslav, Vladimír; Kauppila, Tiina J.; Cvačka, Josef; Kostiainen, Risto

doi:10.1016/j.aca.2015.03.029

Cited by 12 publications

(5 citation statements)

References 45 publications

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“…47 Previous studies on the DESI analysis of drugs in urine samples recommended the use of a solvent mixture of methanol : water (50 : 50) and nebulizing gas pressures between 10 and 12 bar. 14,15,22,30,48 In the present work, the percentage of methanol had to be increased up to 80% (methanol : water 80 : 20) and the gas pressure had to be decreased down to 8-9 bar to allow the formation of a more stable electrospray plume, as well as to help prevent solvent/sample splashing and to avoid potential contamination and carryover at the DESI source. It should also be mentioned that the addition of 0.1% formic acid to the spray solvent did not increase the ionization efficiency of stimulants in positive ion mode.…”

Section: Evaluation Of Desi-hrmsmentioning

confidence: 99%

“…These techniques have been proposed for the analysis of different families of drugs in complex biological fluids such as urine and blood, mainly in pharmacology and forensic applications. 8,13–35 In the field of doping control, DESI has been used for the analysis of clenbuterol in urine pretreated with solid phase extraction 26 and for the analysis of anabolic steroids in urine by reactive DESI. 22 PSI has been applied to the analysis and/or quantitation of a couple of stimulants, 13,21 a combination of two steroids and two diuretics 18 and a limited number of very polar compounds.…”

Section: Introductionmentioning

confidence: 99%

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Potential of desorption electrospray ionization and paper spray ionization with high-resolution mass spectrometry for the screening of sports doping agents in urine

et al. 2023

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Section: Evaluation Of Desi-hrmsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Potential of desorption electrospray ionization and paper spray ionization with high-resolution mass spectrometry for the screening of sports doping agents in urine

et al. 2023

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“…In 2007, Huang et al developed one reactive DESI method by adding a derivatization reagent in spray solvent for the fast screening of anabolic steroids in urine . Compared with ESI, atmospheric pressure photoionization (APPI), as another soft ionization method, has favorable advantages such no polarity discrimination and reduced ion suppression characteristics, − and thus, APPI has attracted increasing attention in the steroid analysis. , Previously, desorption APPI, one typical ambient ionization method derived from APPI, was applied to the fast analysis of urinary steroids during pregnancy, but the steroid quantification ability was not tested.…”

Section: Introductionmentioning

confidence: 99%

Rapid Quantification of Endogenous Steroids in Human Serum Using Leidenfrost Effect-Assisted Thermal Desorption Atmospheric Pressure Photoionization Orbitrap Mass Spectrometry

Zhao

Zhang

Feng

et al. 2022

J. Am. Soc. Mass Spectrom.

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Unconjugated sex steroids in human serum play a crucial role in physiological and pathological studies and are frequently considered as biomarkers in clinical diagnosis. Because of their low polarity, poor volatility, and low concentration, the rapid and highly sensitive analysis of sex steroids in real serum matrix by ambient mass spectrometry is still challenging. Here, Leidenfrost effect-assisted thermal desorption atmospheric pressure photoionization orbitrap mass spectrometry (LETD-APPI-MS) was developed and applied to quantify free sex steroids in human serum without derivatization and chromatography separation within a few minutes. The concentration of target analyte could be increased by approximately two orders during the LETD process. The limit of quantifications and detections of endogenous sex steroids in human serum were measured at the ppt level. In contrast with commonly used immunoassays in clinical laboratories, LETD-APPI-MS enables the accurate measurements of multiple free sex steroids without the interference of cross-reactions. The endogenous sex steroids of 38 female serums at four physiological stages during pregnancy were rapidly tested by LETD-APPI-MS, whose results were highly consistent with that using liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (LC-APCI-MS), indicating LETD-APPI-MS has a strong clinical application potential in steroid analysis.

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“…Several previous studies have shown application of analysis of urinary steroid metabolites to determine pregnancy status in various species [36,49,84,85], including human [86]. Both bovine blood and bovine CL tissue can convert P4 to 20β-hydroxypregn-4-en-3-one [87] that can be determined in urine.…”

Section: P4 Determination In Pregnant Cattlementioning

confidence: 99%

Serum, milk, saliva and urine progesterone and estradiol profiles in crossbred (Zebu x Holstein Friesian) dairy cattle

Mekonnin¹,

Howie²,

Riley³

et al. 2017

Anim Husb Dairy Vet Sci

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Effective breeding management is a crucial tool to enhance the reproductive and productive performance of dairy cattle. To achieve this, regular monitoring of their reproductive status is important. The aims of this study were to monitor the reproductive status of crossbred dairy cattle based on progesterone (P4) concentration in serum, whole milk, saliva and urine, and estradiol in serum using quantitative laboratory ELISA tests, and to document hormonal profiles at different reproductive stages. Three hundred and thirty-six (336) crossbred (Holstein Friesian x Zebu) dairy cattle (232 cows and 104 heifers) from 47 dairy farms owned by smallholder or organized commercial dairy farmers were included in the study. Matched blood, milk and saliva samples were collected twice at 11-day intervals at afternoon milking, while urine was collected just once. Serum was separated within 48 hr of blood collection. All samples were stored at -80 o C until assayed. In both cows and heifers, P4 was higher during pregnancy than in other reproductive conditions. Estradiol was higher during estrus than at other reproductive stages. Estradiol levels were significantly higher (p<0.05) in estrus cows compared with heifers. The mean level of P4 in serum strongly correlated with the value in milk (r = 0.6368, P< 0.0001), however there was no correlation in P4 concentration between serum and saliva or between P4 in serum and in urine. Hence, determination of P4 in serum and milk using an ELISA test is a reliable and precise method to monitor reproductive status in crossbred dairy cattle.

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Feasibility of desorption atmospheric pressure photoionization and desorption electrospray ionization mass spectrometry to monitor urinary steroid metabolites during pregnancy

Cited by 12 publications

References 45 publications

Potential of desorption electrospray ionization and paper spray ionization with high-resolution mass spectrometry for the screening of sports doping agents in urine

Potential of desorption electrospray ionization and paper spray ionization with high-resolution mass spectrometry for the screening of sports doping agents in urine

Rapid Quantification of Endogenous Steroids in Human Serum Using Leidenfrost Effect-Assisted Thermal Desorption Atmospheric Pressure Photoionization Orbitrap Mass Spectrometry

Serum, milk, saliva and urine progesterone and estradiol profiles in crossbred (Zebu x Holstein Friesian) dairy cattle

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