Feasibility of chitosan-based hyaluronic acid hybrid biomaterial for a novel scaffold in cartilage tissue engineering

Yamane, Shintaro; Iwasaki, Norimasa; Majima, Tokifumi; Funakoshi, Tadanao; Masuko, Tatsuya; Harada, Kaoru; Minami, Akio; Monde, Kenji; Nishimura, Shin‐Ichiro

doi:10.1016/j.biomaterials.2004.03.013

Cited by 268 publications

(190 citation statements)

References 39 publications

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“…This seems to be contradictory to the fact that increased cell penetration was observed in scaffolds containing HA. The result also contrasted to previous observations suggesting a mitogenic effect of HA in scaffolds [29,30,45]. In our case, we suggest that the observed result may be based on the accumulation of cells on the surface of the control SF:HA (100:0) scaffolds that could compensate for the reduced population in the core.…”

Section: Scaffold Design: Composition and Microstructurecontrasting

confidence: 56%

“…While several reports indicate that HA induces expression of this structural protein [27,48,49], others observed downregulation when cells were cultured on HA scaffolds [25]. The general tendency is that scaffolds based on HA favor proliferation [50], inhibit the production of proteins that mediate cell attachment [25], up-regulate the expression of cartilage related genes [27,30,48,49,51], and modulate the expression of inflammatory and catabolic markers [49,51,52]. The relation between HA composition, bone related genes and extracellular matrix components (e.g.…”

Section: Scaffold Design: Composition and Microstructurementioning

confidence: 99%

“…For tissue engineering applications, HA and HA-based scaffolds have been investigated for the repair of ligament [25], cartilage [26][27][28][29][30], adipose tissue [31], bone [32] and osteochondral defects [33,34]. There were even some clinical tests on the efficacy of HA scaffolds for the tissue engineering of cartilage [27,35], and for artery regeneration [36].…”

Section: Introductionmentioning

confidence: 99%

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Silk fibroin/hyaluronan scaffolds for human mesenchymal stem cell culture in tissue engineering

et al. 2009

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Section: Scaffold Design: Composition and Microstructurecontrasting

confidence: 56%

Section: Scaffold Design: Composition and Microstructurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Silk fibroin/hyaluronan scaffolds for human mesenchymal stem cell culture in tissue engineering

et al. 2009

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“…Structurally, chitosan is a linear polysaccharide consisting of β(1→4) linked D-glucosamine residues with a variable number of randomly located N-acetyl-glucosamine groups. The average molecular weight ranges from 50 to 1.000 kDa 5 . Chitosan had not a specific binding domain for cell adhesion mediated by integrin receptors.…”

Section: Cell Morphology By Light Microscopymentioning

confidence: 99%

“…Chitosan is a partially deacetylated derivative of chitin, which is the second more abundant polysaccharide found in nature and the primary structural polymer in arthropod exoskeletons 5 . Chitosan has generated enormous interest as a biomaterial due to its various advantages, such as (1) low cost, (2) easy availability, (3) biocompatibility and (4) anti-microbial activity 6 .…”

Section: Introductionmentioning

confidence: 99%

Sorbitol-Plasticized and Neutralized Chitosan Membranes as Skin Substitutes

2015

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Chitosan is soluble in diluted acid solutions and can easily form films by casting. However, residual acid neutralization should be performed for biomedical applications what may compromise physical and mechanical properties of the films. Thus, plasticizers can be added to improve these properties. The aim of this study was to characterize morphological, barrier and mechanical properties, besides evaluate the in vitro cytotoxicity of sorbitol-plasticized and NaOH-Na 2 CO 3 neutralized chitosan membranes for skin substitute application. Scanning electron microscopy, X-ray diffraction, water vapor permeability and mechanical tests were carried out to characterize the obtained membranes. Moreover, Vero cells were used for in vitro cytotoxicity evaluation. In this paper, we report a non-cytotoxic sorbitol-plasticized chitosan membrane with desirable properties for skin substitution, such as flexibility, water vapor permeability and high percentage of elongation.

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Biomaterials: An Overview

Seal

Panitch

2006

Encyclopedia of Medical Devices and Instrumentation

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Biomaterials are materials of metallic, ceramic, polymeric, or composite origin that are used or that have been designed for use in medical devices or in contact with the body in order to augment, replace, or restore the function of diseased or damaged tissues or organs. Applications involving biomaterials range from load bearing orthopedic devices to degradable sutures to living skin equivalents. Although the types of materials used as biomaterials is diverse, most implanted biomaterials elicit a very similar inflammatory and foreign body response. In an attempt to minimize adverse biological responses and improve the performance of biomaterials at a tissue, cellular, and molecular level, current research in biomaterials has led to a paradigm change in which biomaterials consist of, mimic, or are designed to interact specifically with biological elements and systems. Continued adaptation of biomaterials design to biological responses will result in better performing devices that work with the body to promote tissue regeneration and healing.

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Feasibility of chitosan-based hyaluronic acid hybrid biomaterial for a novel scaffold in cartilage tissue engineering

Cited by 268 publications

References 39 publications

Silk fibroin/hyaluronan scaffolds for human mesenchymal stem cell culture in tissue engineering

Silk fibroin/hyaluronan scaffolds for human mesenchymal stem cell culture in tissue engineering

Sorbitol-Plasticized and Neutralized Chitosan Membranes as Skin Substitutes

Biomaterials: An Overview

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