Feasibility of chemohyperthermia with docetaxel‐embedded magnetoliposomes as minimally invasive local treatment for cancer

Abstract

Hyperthermia is a minimally invasive approach to cancer treatment, but it is difficult to heat only the tumor without damaging surrounding tissue. To solve this problem, we studied the effectiveness of chemohyperthermia with docetaxel-embedded magnetoliposomes (DMLs) and an applied alternating current (AC) magnetic field. Human MKN45 gastric cancer cells were implanted in the hind limb of Balb-c/nu/nu mice. Various concentrations of docetaxel-embedded DMLs were injected into the tumors and exposed to an AC magnetic field (n 5 6, each). For comparison with hyperthermia alone, magnetite-loaded liposome (ML)-injected tumors were exposed to an AC magnetic field. Furthermore, the results of DML without AC treatment and docetaxel diluted into PBS with AC treatment were also compared (n 5 10, each). Tumor surface temperature was maintained between 42 and 43°C. Tumor volume was reduced in the DML group with a docetaxel concentration > 56.8 lg/ml, while a docetaxel concentration > 568.5 lg/ml was required for tumor reduction without hyperthermia. Statistically significant differences in tumor volume and survival rate were observed between the DML group exposed to the magnetic field and the other groups. The tumor disappeared in 3 mice in the DML group exposed to the magnetic field; 2 mice survived over 6 months after treatment, whereas all mice of the other groups died by 15 weeks. Histologically, hyperthermia with DML damaged tumor cells and DML diffused homogeneously. To the best of our knowledge, this is the first report to show that hyperthermia using chemotherapeutic agent-embedded magnetoliposomes has an anticancer effect.

Feasibility of tailored, selective and effective anticancer chemotherapy by direct injection of docetaxel-loaded immunoliposomes into Her2/neu positive gastric tumor xenografts

“…Thus, many Berlin Blue-staining positive magnetic particles were observed in the regional lymph nodes within one day after injection (29). These data indicate that local injection of anticancer drug-containing liposomes of a small size may simultaneously treat regional metastatic lymph nodes and the primary tumor.…”

Application of liposomes in drug development – focus on gastroenterological targets

“…77,78 There were also attempts to explore other types of liposomes for treating GI cancer to enhance tumor specific drug delivery. 79,80 Injecting docetaxel embedded magnetoliposomes into human MKN45 gastric cancer bearing mice, followed by exposure to an alternating current or magnetic field and local hyperthermia, produced positive results where the amount of drug required for inhibiting cancer growth was greatly reduced, the size of the tumor was decreased, and the survival time was prolonged. 79 Ultrasound was also utilized to trigger cisplatin release from sterically stabilized liposomes and almost 70% of liposomal cisplatin was released in tumor tissue, resulting in tumor regression.…”

Heat generation properties in AC magnetic field for composite powder material of the Y3Fe5O12–nSiC system prepared by reverse coprecipitation method

“…The heat generation ability in an AC magnetic field for magnetic materials has been studied for the local treatment of cancerous tissues [1][2][3][4][5][6][7]. Materials of the needle type and powder type have been studied for this purpose.…”