2021
DOI: 10.1088/1741-2552/abef89
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Feasibility of cerium-doped LSO particles as a scintillator for x-ray induced optogenetics

Abstract: Optogenetics is a widely used tool for studying neural circuits. However, non-invasive methods for light delivery in the brain are needed to avoid physical damage typically caused by intracranial insertion of light guides. An innovative strategy could employ X-ray activation of radioluminescent particles (RLPs) to emit localized light. We previously reported that RLPs composed of cerium doped lutetium oxyorthosilicate (LSO:Ce), an inorganic scintillator that emits blue light, are biocompatible with neuronal fu… Show more

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Cited by 13 publications
(9 citation statements)
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References 78 publications
(151 reference statements)
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“…One of the few studies on the topic to date by French et al . observed that light emitted from Y2(SiO 4 )O:Ce nanoparticles in response to UV stimulation caused an increase in the frequency of spontaneous excitatory postsynaptic currents (sEPSCs) in CA1 pyramidal cells expressing the light sensitive opsin channelrhodopsin-2 (ChR2)—commonly used for optogenetic experiments [ 39 41 ]. Interestingly, the stimulation paradigm used for these experiments was insufficient to change sEPSC amplitude or evoke light-gated action potentials, consistent with the relatively small photocurrents induced.…”
Section: Discussionmentioning
confidence: 99%
“…One of the few studies on the topic to date by French et al . observed that light emitted from Y2(SiO 4 )O:Ce nanoparticles in response to UV stimulation caused an increase in the frequency of spontaneous excitatory postsynaptic currents (sEPSCs) in CA1 pyramidal cells expressing the light sensitive opsin channelrhodopsin-2 (ChR2)—commonly used for optogenetic experiments [ 39 41 ]. Interestingly, the stimulation paradigm used for these experiments was insufficient to change sEPSC amplitude or evoke light-gated action potentials, consistent with the relatively small photocurrents induced.…”
Section: Discussionmentioning
confidence: 99%
“…Although the idea of X-optogenetics was first proposed by Berry et al in 2015, it was not until six years later that studies were performed and reported to prove its efficiency. Bartley et al applied Ce-doped Lu 2 SiO 5 nanoparticles (LSO:Ce) for in vitro X-optogenetics in 2021 . After X-ray activation, the LSO:Ce nanoparticles created a broad radioluminescence emission ranging from 370 to 590 nm, which includes the peak absorption wavelength of ChR2.…”
Section: Application Of Nanoparticles In Wireless Phototherapymentioning
confidence: 99%
“…Bartley et al applied Ce-doped Lu 2 SiO 5 nanoparticles (LSO:Ce) for in vitro X-optogenetics in 2021. 64 After X-ray activation, the LSO:Ce nanoparticles created a broad radioluminescence emission ranging from 370 to 590 nm, which includes the peak absorption wavelength of ChR2. Significantly enhanced spontaneous excitatory postsynaptic currents (sEPSCs) were recorded from neurons that were exposed to both LSO:Ce and X-rays, proving the potential of using LSO:Ce as nanoscintillators in X-optogenetics.…”
Section: X-ray Nanoscintillatorsmentioning
confidence: 99%
“…Successful activation and inhibition of midbrain dopamine neurons were implemented in free-moving mice by injecting X-ray luminescence nanoprobes to induce the generation of bidirectional regulation of location-preference behavior in mice. In addition, X-ray-luminescence-mediated optogenetic regulation was confirmed by other groups, offering an attractive tool to take the practical implementation of optogenetic neural control toward minimally invasive wireless deep brain stimulation.…”
Section: X-ray-luminescence-mediated Optogenetic Neuromodulationmentioning
confidence: 99%