Feasibility and mechanism of light-driven nitrogen reduction to ammonium by a &amp;lt;italic&amp;gt;Pseudomonas stutzeri&amp;lt;/italic&amp;gt;-CdS semi-artificial photosynthetic system

Huang, Shaofu; Jing, Xianyue; Chen, Man; Zhou, Shungui

doi:10.1360/sst-2020-0238

Cited by 3 publications

(5 citation statements)

References 14 publications

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“…However, the yield of NH 4 + did not increase consistently because nitrogenase was relatively inactive when there was sufficient NH 4 + in the reaction system 29 . To the best of our knowledge, the NH 4 + production for every day in this study is higher than that reported in most of the literature (Table 1), such as 0.92 times that of a single‐chamber bioanode MEC system, 17 3.15 times that of a dual‐chamber e‐BNF system 18 and 1.54 times that of a semi‐artificial photosynthetic system 13 . It is much higher than the N 2 fixation activity of pure culture system (3.5 times) 12 .…”

Section: Resultscontrasting

confidence: 49%

“…29 To the best of our knowledge, the NH 4 + production for every day in this study is higher than that reported in most of the literature (Table 1), such as 0.92 times that of a single-chamber bioanode MEC system, 17 3.15 times that of a dual-chamber e-BNF system 18 and 1.54 times that of a semi-artificial photosynthetic system. 13 It is much higher than the N 2 fixation activity of pure culture system (3.5 times). 12 Moreover, the results of three groups of control experiments indicated that E AP = 1.5 V, 40 °C and N 2 atmosphere were indispensable for N 2 fixation experiments in the biocathode reaction system, which were direct evidence that NH 3 originated from microbial electrochemical processes.…”

Section: Resultsmentioning

confidence: 89%

“…11 Chen et al and Huang et al reported significant N 2 fixation performance through pure culture or semi-artificial photosynthetic system. 12,13 However, these systems all face serious stability problems and low efficiency of NH 3 production, and are difficult to operate stably for a long time in the catalytic environment.…”

Section: Introductionmentioning

confidence: 99%

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Investigating the performance and mechanism of nitrogen gas fixation and conversion to ammonia based on biocathode bioelectrochemistry system

Lin

Guo

et al. 2022

J of Chemical Tech & Biotech

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Background Nowadays, microbial electrochemistry is widely used to produce valuable products (CH4, CH3CH2OH, CH3COOH, etc.) from carbon dioxide. Meanwhile, given that low‐value nitrogen (N2) is abundantly available as a feedstock for chemicals, thus ammonia (NH3) synthesis using bioelectrochemistry is still a promising technology. However, considering N2 stability and ammonium (NH4+) adsorption with a proton exchange membrane, developing suitable microbial systems for NH3 synthesis still faces challenges. Based on this, we developed a single‐chamber biocathode bioelectrochemical system for efficient NH3 production. Results For the reaction system that we constructed, NH4+ production reached 6.31 mg L−1 within 10 days, and NH4+ production was positively correlated with current density. A C2H2 reduction assay further verified the activity of nitrogenase enzyme. Microbial community analysis revealed that Clostridia plays a crucial role in the N2 fixation process, and the synergistic interaction between different microorganisms favors electron transfer and an increase in NH3 production. Conclusions The results of a series of experiments have proved that this green and economical method can be used to efficiently generate NH4+. This reaction system not only utilizes the advantages of obtaining electrons for reduction reaction, but also improves electron transfer and avoids the shortcomings of NH4+ adsorption. Additionally, this in‐depth study of N2 fixation mechanism provides a theoretical reference for other applications of bioelectrochemical systems. © 2022 Society of Chemical Industry (SCI).

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Section: Resultscontrasting

confidence: 49%

Section: Resultsmentioning

confidence: 89%

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Investigating the performance and mechanism of nitrogen gas fixation and conversion to ammonia based on biocathode bioelectrochemistry system

Lin

Guo

et al. 2022

J of Chemical Tech & Biotech

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“…The concentration of NO 3 – -N was determined by dual-wavelength spectrophotometry (Shimadzu UV2600) . The concentrations of NO 2 – -N and NH 4 + -N were measured according to N -(1-naphthyl)-ethylenediamine spectrophotometry and the indophenol blue colorimetric method, respectively. , The N 2 O–N concentration in the headspace was analyzed using a gas chromatograph (GC, Agilent 7890, USA) equipped with an electron capture detector (ECD); the details of the procedures were described in a previous study . The concentration of N 2 –N was calculated based on the relationship of nitrogen balance.…”

Section: Methodsmentioning

confidence: 99%

“…In addition to denitrifiers, a series of soil microbial processes, including nitrogen fixation, hydrogen production, and carbon fixation, have been demonstrated to undergo photoelectrotrophic pathways in model systems. 27,59 As they widely occur in soil, the results of this work also imply the possibility of these processes being driven by sunlight. More work to demonstrate these processes should to be done in the future to better understand the biogeochemical processes of elements in soil.…”

Section: Effects Of Irradiation On the Structure Of The Microbial Com...mentioning

confidence: 99%

Sunlight Significantly Enhances Soil Denitrification via an Interfacial Biophotoelectrochemical Pathway

Huang

Chen

et al. 2023

Environ. Sci. Technol.

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Denitrification is an essential step of the nitrogen cycle in soil. However, although sunlight is an important environmental factor for soil, the investigation of the influence of sunlight on soil denitrification is limited to plant photosynthesis-mediated processes. Herein, a new pathway, denoted as a biophotoelectrochemical process, which is induced by the direct photoexcitation of soil, was found to greatly enhance soil denitrification. Using red soil as the research object, the soil with irradiation showed nitrate reduction that was 2.6–4.7 times faster than that without irradiation. The irradiation of soil accelerated the reduction of nitrite and enhanced the conversion of nitrous oxide to nitrogen, indicating that more electron sources were generated. This resulted from the photoinduced generation of ferrous substrates and photoelectrons. The contribution of irradiation to soil denitrification was almost half (45.4%), of which 30.9% was from photoinduced ferrous substrates and 14.5% was from photoelectrons. Moreover, a designed biophotoelectrochemical cell provided solid evidence for direct photoelectron transfer from soil photosensitive substrates to microorganisms. Irradiation promoted the enrichment of Alicyclobacillus, which participates in iron oxidation and electroautotrophy. This finding reveals a role of sunlight in soil denitrification that has been thus seriously overlooked and provides solid evidence for the natural occurrence of photoelectrotrophic effects.

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Constructing a semi-artificial photosynthetic system for the removal of trichloroethylene by visible light-driven obligate organohalide-respiring bacteria

Tang¹,

Tang²,

Jiang³

et al. 2022

Chin. Sci. Bull.

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Feasibility and mechanism of light-driven nitrogen reduction to ammonium by a <italic>Pseudomonas stutzeri</italic>-CdS semi-artificial photosynthetic system

Cited by 3 publications

References 14 publications

Investigating the performance and mechanism of nitrogen gas fixation and conversion to ammonia based on biocathode bioelectrochemistry system

Investigating the performance and mechanism of nitrogen gas fixation and conversion to ammonia based on biocathode bioelectrochemistry system

Sunlight Significantly Enhances Soil Denitrification via an Interfacial Biophotoelectrochemical Pathway

Constructing a semi-artificial photosynthetic system for the removal of trichloroethylene by visible light-driven obligate organohalide-respiring bacteria

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