Feasibility and Acceptability of a Novel Cervicovaginal Lavage Self-Sampling Device Among Women in Kigali, Rwanda

Ndayisaba, Gilles; Verwijs, Marijn C.; Eeckhoudt, S. Van; Gasarabwe, Ammiel; Hardy, Liselotte; Borgdorff, Hanneke; Kestelyn, Evelyne; Jespers, Vicky; Wijgert, Janneke van de

doi:10.1097/olq.0b013e31828e5aa5

Cited by 19 publications

(19 citation statements)

References 17 publications

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“…The findings on the feasibility of self-sampling are in line with a study from Rwanda, where women practiced doing the self-sampling with the presence of a clinician in order to maximise feasibility [24]. However, the results are somewhat in contrast to two randomised controlled trials from Nigeria and South Africa [25,29].…”

Section: Discussionsupporting

confidence: 78%

“…However, some studies also reported concerns in relation to selfsampling, i.e. not trusting the HPV results [32] as well as feeling nervous, insecure, or inexperienced [24]. To the authors' knowledge, there is no qualitative study of how Tanzanian women perceive HPV self-sampling.…”

Section: Introductionmentioning

confidence: 92%

“…European studies have shown that women from Germany (89%), Netherlands (91%) and Italy (94%) perceived self-sampling as easy [19][20][21], and women from the Netherlands (75%), Italy (78%) and the US (79%) preferred self-sampling over a provider-based examination [21][22][23]. Research on feasibility and acceptability of HPV self-sampling in Africa is limited, however a few studies have been conducted in Rwanda, Uganda, Kenya, South Africa, Cameroon, and Nigeria [24][25][26][27][28][29][30][31][32][33]. Overall, self-sampling was found to be an acceptable and feasible screening method.…”

Section: Introductionmentioning

confidence: 99%

“…Overall, self-sampling was found to be an acceptable and feasible screening method. For example, questionnaire surveys from Rwanda and Uganda showed that 70 and 80% of women preferred selfsampling over provider-sampling [24,26] and mixed method studies from Rwanda, South Africa, and Nigeria found it to be a feasible method [24,29,33]. However, some studies also reported concerns in relation to selfsampling, i.e.…”

Section: Introductionmentioning

confidence: 99%

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“The best thing is that you are doing it for yourself” – perspectives on acceptability and feasibility of HPV self-sampling among cervical cancer screening clients in Tanzania: a qualitative pilot study

et al. 2020

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Background: Cervical cancer is the most common type of cancer in sub-Saharan Africa, and it is also the cancer disease that most women die from. The high mortality rate is partly due to low attendance rates to screening services and low sensitivity of visual inspection with acetic acid, which is the standard screening method used in screening programs in sub-Saharan Africa. In order to overcome of the burden of disease new screening strategies and methods are warranted. This study aims to explore the acceptability and feasibility of HPV self-sampling compared to provider-based sampling among cervical cancer screening clients living in Dar es Salaam. Methods: Women attending cervical cancer screening at Ocean Road Cancer Institute in Dar es Salaam, Tanzania between February-April 2017 were invited into the study. The participants had (1) a provider-collected sample, and (2) a self-sample for HPV on top of the regular cervical cancer screening. 50% of the participants conducted the self-sample after receiving a written instruction guide of how to collect the sample (written). The other 50% received both the written and an oral introduction to self-sampling (written+). All participants could ask for nurse assistance during self-sample collection if needed. Individual semi-structured interviews were conducted with the participants post sample collection. Data collection stopped when saturation was reached. Data were analysed using a thematic content analysis.

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Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

“The best thing is that you are doing it for yourself” – perspectives on acceptability and feasibility of HPV self-sampling among cervical cancer screening clients in Tanzania: a qualitative pilot study

et al. 2020

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“…CVL is a safe and simple procedure that can be carried out easily in the clinic and self-sampling by trial participants also has been reported. A new self-sampling device was evaluated by Rwandan women and found to be acceptable for CVL collection [3], increasing the potential for sample collection in longitudinal studies.…”

Section: Introductionmentioning

confidence: 99%

Accurate measurement of female genital tract fluid dilution in cervicovaginal lavage samples

Churchman

Moss

Baum

2016

Journal of Chromatography B

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An ion chromatographic method with conductivity detection for the precise and accurate analysis of lithium ions in phosphate-buffered saline, used as a cervicovaginal lavage (CVL) fluid, was developed and validated. The lithium ion dilution factor during the CVL is used to calculate the volume of cervicovaginal fluid (CVF) collected. Initial CVL Li+ concentrations of 1 mM and 10 mM were evaluated. The method is robust, practical, and afforded an accurate measurement (5% of the measurement, or better) at 24 μL of vaginal fluid simulant collected per mL of CVL fluid, as low as 5 μL mL−1 using 10 mM Li+ with a measurement accuracy of 6.7%. Ion chromatograms of real-world CVL samples collected in vivo from common animal models (sheep and pig-tailed macaque) and a human volunteer demonstrate that the analysis is interference-free. The method is readily transferrable and should enable the accurate measurement of CVF volume collected during CVLs benefitting a broad range of research disciplines, including pharmacokinetic, pharmacodynamic, metabolomic, and microbiome studies.

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Intricacies of assessing the human microbiome in epidemiologic studies

Robinson

Brotman

Ravel

2016

Annals of Epidemiology

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Purpose In the past decade, remarkable relationships have been documented between dysbiosis of the human microbiota and adverse health outcomes. This review seeks to highlight some of the challenges and pitfalls that may be encountered during all stages of microbiota research, from study design and sample collection, to nucleic acid extraction and sequencing, and bioinformatic and statistical analysis. Methods Literature focused on human microbiota research was reviewed and summarized. Results While most studies have focused on surveying the composition of the microbiota, fewer have explored the causal roles of these bacteria, archaea, viruses, and fungi in affecting disease states. Microbiome research is in its relatively early years and many aspects remain challenging, including the complexity and personalized aspects of microbial communities, the influence of exogenous and often confounding factors, the need to apply fundamental principles of ecology and epidemiology, the necessity for new software tools, and the rapidly evolving genomic, technological, and analytical landscapes. Conclusions Incorporating human microbiome research in large epidemiological studies will soon help us unravel the intricate relationships that we have with our microbial partners and provide interventional opportunities to improve human health.

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Feasibility and Acceptability of a Novel Cervicovaginal Lavage Self-Sampling Device Among Women in Kigali, Rwanda

Cited by 19 publications

References 17 publications

“The best thing is that you are doing it for yourself” – perspectives on acceptability and feasibility of HPV self-sampling among cervical cancer screening clients in Tanzania: a qualitative pilot study

“The best thing is that you are doing it for yourself” – perspectives on acceptability and feasibility of HPV self-sampling among cervical cancer screening clients in Tanzania: a qualitative pilot study

Accurate measurement of female genital tract fluid dilution in cervicovaginal lavage samples

Intricacies of assessing the human microbiome in epidemiologic studies

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