Fe3O4@M nanoparticles for MRI-targeted detection in the early lesions of atherosclerosis

Huang, Xin; Lin, Chenyu; Luo, Cici; Guo, Yu-Han; Li, Jun; Wang, Yiping; Xu, Jiahong; Zhang, Yuwen; Wang, He; Liu, Zhongmin; Chen, Bingdi

doi:10.1016/j.nano.2020.102348

Cited by 22 publications

(21 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…H&E staining was performed on their hearts, livers, spleens, lungs, and kidneys. Pathological changes were observed by optical microscopy [ 14 , 16 ].…”

Section: Methodsmentioning

confidence: 99%

CKAP4 Antibody-Conjugated Si Quantum Dot Micelles for Targeted Imaging of Lung Cancer

Huang

Chen

et al. 2021

Nanoscale Res Lett

Self Cite

View full text Add to dashboard Cite

At present, various fluorescent nanomaterials have been designed and synthesized as optical contrast agents for surgical navigation. However, there have been no reports on the preparation of fluorescent contrast agents for lung cancer surgery navigation using silicon quantum dots (Si QDs). This study improved and modified the water-dispersible Si QD micelles reported by Pi et al. to prepare Si QD micelles-CKAP4. The data showed that the Si QD micelles-CKAP4 were spherical particles with a mean hydrodiameter of approximately 78.8 nm. UV–visible absorption of the Si QD micelles-CKAP4 ranged from 200 to 500 nm. With an excitation wavelength of 330 nm, strong fluorescence at 640 nm was observed in the fluorescence emission spectra. Laser confocal microscopy and fluorescence microscopy assay showed that the Si QD micelles-CKAP4 exhibited good targeting ability to lung cancer cells and lung cancer tissues in vitro. The in vivo fluorescence-imaging assay showed that the Si QD micelles-CKAP4 was metabolized by the liver and excreted by the kidney. In addition, Si QD micelles-CKAP4 specifically targeted lung cancer tissue in vivo compared with healthy lung tissue. Cytotoxicity and hematoxylin and eosin staining assays showed that the Si QD micelles-CKAP4 exhibited high biosafety in vitro and in vivo. Si QD micelles-CKAP4 is a specifically targeted imaging agent for lung cancer and is expected to be a fluorescent contrast agent for lung cancer surgical navigation in the future.

show abstract

“…H&E staining was performed on their hearts, livers, spleens, lungs, and kidneys. Pathological changes were observed by optical microscopy [ 14 , 16 ].…”

Section: Methodsmentioning

confidence: 99%

CKAP4 Antibody-Conjugated Si Quantum Dot Micelles for Targeted Imaging of Lung Cancer

Huang

Chen

et al. 2021

Nanoscale Res Lett

Self Cite

View full text Add to dashboard Cite

show abstract

“…Effectively target early atherosclerotic lesions 3. Bioimaging by MRI [ 110 ] RAW264.7 PLGA Rapamycin In vitro: HUVEC, smooth muscle cells and RAW264.7 In vivo: ApoE −/− mice 1. Good biocompatibility 2.…”

Section: Application Of Macrophage Membrane Biomimetic Nanoparticlesmentioning

confidence: 99%

Macrophage cell membrane-based nanoparticles: a new promising biomimetic platform for targeted delivery and treatment

Wan

Yang

et al. 2022

J Nanobiotechnol

View full text Add to dashboard Cite

Synthetic nanoparticles with surface bioconjugation are promising platforms for targeted therapy, but their simple biological functionalization is still a challenging task against the complex intercellular environment. Once synthetic nanoparticles enter the body, they are phagocytosed by immune cells by the immune system. Recently, the cell membrane camouflage strategy has emerged as a novel therapeutic tactic to overcome these issues by utilizing the fundamental properties of natural cells. Macrophage, a type of immune system cells, plays critical roles in various diseases, including cancer, atherosclerosis, rheumatoid arthritis, infection and inflammation, due to the recognition and engulfment function of removing substances and pathogens. Macrophage membranes inherit the surface protein profiles and biointerfacing properties of source cells. Therefore, the macrophage membrane cloaking can protect synthetic nanoparticles from phagocytosis by the immune cells. Meanwhile, the macrophage membrane can make use of the natural correspondence to accurately recognize antigens and target inflamed tissue or tumor sites. In this review, we have summarized the advances in the fabrication, characterization and homing capacity of macrophage membrane cloaking nanoparticles in various diseases, including cancers, immune diseases, cardiovascular diseases, central nervous system diseases, and microbial infections. Although macrophage membrane-camouflaged nanoparticles are currently in the fetal stage of development, there is huge potential and challenge to explore the conversion mode in the clinic.

show abstract

“…Iron oxide superparamagnetic nanoparticles (Fe 3 O 4 MNPs) have attracted considerable scientific interest due to their superparamagnetic properties, biocompatibility, and non-toxicity, resulting in a wide range of biomedical and technological applications. For example, Fe 3 O 4 MNPs have been applied in energy storage [ 1 ]; tissue engineering [ 2 ]; protein, DNA, and cell separation from samples [ 3 , 4 ]; biosensing [ 5 ]; drug-delivery and -targeting [ 6 , 7 , 8 ]; magnetic resonance imaging (MRI) [ 9 , 10 , 11 ]; and as mediators of heat for cancer therapy (hyperthermia) [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Nanozyme-Based Lateral Flow Immunoassay (LFIA) for Extracellular Vesicle Detection

et al. 2022

View full text Add to dashboard Cite

Extracellular vesicles (EVs) are biological nanoparticles of great interest as novel sources of biomarkers and as drug delivery systems for personalized therapies. The research in the field and clinical applications require rapid quantification. In this study, we have developed a novel lateral flow immunoassay (LFIA) system based on Fe3O4 nanozymes for extracellular vesicle (EV) detection. Iron oxide superparamagnetic nanoparticles (Fe3O4 MNPs) have been reported as peroxidase-like mimetic systems and competent colorimetric labels. The peroxidase-like capabilities of MNPs coated with fatty acids of different chain lengths (oleic acid, myristic acid, and lauric acid) were evaluated in solution with H2O2 and 3,3,5,5-tetramethylbenzidine (TMB) as well as on strips by biotin–neutravidin affinity assay. As a result, MNPs coated with oleic acid were applied as colorimetric labels and applied to detect plasma-derived EVs in LFIAs via their nanozyme effects. The visual signals of test lines were significantly enhanced, and the limit of detection (LOD) was reduced from 5.73 × 107 EVs/μL to 2.49 × 107 EVs/μL. Our work demonstrated the potential of these MNPs as reporter labels and as nanozyme probes for the development of a simple tool to detect EVs, which have proven to be useful biomarkers in a wide variety of diseases.

show abstract

Fe3O4@M nanoparticles for MRI-targeted detection in the early lesions of atherosclerosis

Cited by 22 publications

References 41 publications

CKAP4 Antibody-Conjugated Si Quantum Dot Micelles for Targeted Imaging of Lung Cancer

CKAP4 Antibody-Conjugated Si Quantum Dot Micelles for Targeted Imaging of Lung Cancer

Macrophage cell membrane-based nanoparticles: a new promising biomimetic platform for targeted delivery and treatment

Nanozyme-Based Lateral Flow Immunoassay (LFIA) for Extracellular Vesicle Detection

Contact Info

Product

Resources

About