Nanozyme-Based Lateral Flow Immunoassay (LFIA) for Extracellular Vesicle Detection

Wang, Baihui; Moyano, Amanda; Duque, José María; Manso, Luis; García-Santos, Guillermo; Flórez, Luis; Serrano-Pertierra, Esther; Blanco‐López, M. Carmen

doi:10.3390/bios12070490

Cited by 9 publications

(7 citation statements)

References 63 publications

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“…Recent research has focused on introducing nanozymes as the detection labels for signal development, with the goal of improving assay perfor-mance and sensitivity. [49][50][51][52] We previously reported an ultrasensitive LFIA for the detection of p24, which is one of the earliest presenting HIV biomarkers, using a porous platinum coreshell structure (Figure 4A). [37] In this immunoassay, antibodyfunctionalized platinum nanocatalysts (PtNCs) displaying high internal surface area owing to an urchin-like morphology served as the detection probe.…”

Section: Nanozymes In Point-of-care Devicesmentioning

confidence: 99%

The Nanozyme Revolution: Enhancing the Performance of Medical Biosensing Platforms

et al. 2023

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Nanozymes represent a class of nanosized materials that exhibit innate catalytic properties similar to biological enzymes. The unique features of these materials have positioned them as promising candidates for applications in clinical sensing devices, specifically those employed at the point‐of‐care. They notably have found use as a means to amplify signals in nanosensor‐based platforms and thereby improve sensor detection limits. Recent developments in the understanding of the fundamental chemistries underpinning these materials have enabled the development of highly effective nanozymes capable of sensing clinically relevant biomarkers at detection limits that compete with “gold‐standard” techniques. However, there remain considerable hurdles that need to be overcome before these nanozyme‐based sensors can be utilized in a platform ready for clinical use. An overview of the current understandings of nanozymes for disease diagnostics and biosensing applications and the unmet challenges that must be considered prior to their translation in clinical diagnostic tests is provided.

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Section: Nanozymes In Point-of-care Devicesmentioning

confidence: 99%

The Nanozyme Revolution: Enhancing the Performance of Medical Biosensing Platforms

et al. 2023

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“…EVs in body fluids are involved in intercellular communication processes and are considered powerful sources of biomarkers for conditions such as acquired immunodeficiency syndrome (AIDS), chronic inflammation, and cancer. [ 96 ] Wang et al [ 61 ] constructed a Fe 3 O 4 MNPs ‐based sensing platform for sensitive detection of EVs. To enhance the stability, biocompatibility, and biocoupling of MNPs in water, double layers of fatty acids were employed.…”

Section: Nanozymes Acting As Signal Amplification Probes For Sensitiv...mentioning

confidence: 99%

“…EVs in body fluids are involved in intercellular communication processes and are considered powerful sources of biomarkers for conditions such as acquired immunodeficiency syndrome (AIDS), chronic inflammation, and cancer. [96] Wang et al [61] The excellent stability, ease of modification, large surface area, and magnetic /optical properties of MNPs enable their multifunctional integration with various nanomaterials or ligands. This capability effectively addresses these limitations of traditional test strips, such as poor matrix tolerance and low sensitivity.…”

Section: Mnps-based Nanozymes Enhanced Sample Enrichment and Color Si...mentioning

confidence: 99%

Signal Amplification Strategy Design in Nanozyme‐Based Biosensors for Highly Sensitive Detection of Trace Biomarkers

Wang,

Liu,

Fan

2023

Small Methods

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Nanozymes show great promise in enhancing disease biomarker sensing by leveraging their physicochemical properties and enzymatic activities. These qualities facilitate signal amplification and matrix effects reduction, thus boosting biomarker sensing performance. In this review, recent studies from the last five years, concentrating on disease biomarker detection improvement through nanozyme‐based biosensing are examined. This enhancement primarily involves the modulations of the size, morphology, doping, modification, electromagnetic mechanisms, electron conduction efficiency, and surface plasmon resonance effects of nanozymes for increased sensitivity. In addition, a comprehensive description of the synthesis and tuning strategies employed for nanozymes has been provided. This includes a detailed elucidation of their catalytic mechanisms in alignment with the fundamental principles of enhanced sensing technology, accompanied by the presentation of quantitatively analyzed results. Moreover, the diverse applications of nanozymes in strip sensing, colorimetric sensing, electrochemical sensing, and surface‐enhanced Raman scattering have been outlined. Additionally, the limitations, challenges, and corresponding recommendations concerning the application of nanozymes in biosensing have been summarized. Furthermore, insights have been offered into the future development and outlook of nanozymes for biosensing. This review aims to serve not only as a reference for enhancing the sensitivity of nanozyme‐based biosensors but also as a catalyst for exploring nanozyme properties and their broader applications in biosensing.

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“…This colorimetric change can be used to quantitatively measure the level of targeted exosomes. MNPs can also be used for direct colorimetric detection of EVs via LFIA using the brown color of the NPs [ 150 ].…”

Section: Magnetic Nanostructuresmentioning

confidence: 99%

Nanomaterials for Molecular Detection and Analysis of Extracellular Vesicles

et al. 2023

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Extracellular vesicles (EVs) have emerged as a novel resource of biomarkers for cancer and certain other diseases. Probing EVs in body fluids has become of major interest in the past decade in the development of a new-generation liquid biopsy for cancer diagnosis and monitoring. However, sensitive and specific molecular detection and analysis are challenging, due to the small size of EVs, low amount of antigens on individual EVs, and the complex biofluid matrix. Nanomaterials have been widely used in the technological development of protein and nucleic acid-based EV detection and analysis, owing to the unique structure and functional properties of materials at the nanometer scale. In this review, we summarize various nanomaterial-based analytical technologies for molecular EV detection and analysis. We discuss these technologies based on the major types of nanomaterials, including plasmonic, fluorescent, magnetic, organic, carbon-based, and certain other nanostructures. For each type of nanomaterial, functional properties are briefly described, followed by the applications of the nanomaterials for EV biomarker detection, profiling, and analysis in terms of detection mechanisms.

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Nanozyme-Based Lateral Flow Immunoassay (LFIA) for Extracellular Vesicle Detection

Cited by 9 publications

References 63 publications

The Nanozyme Revolution: Enhancing the Performance of Medical Biosensing Platforms

The Nanozyme Revolution: Enhancing the Performance of Medical Biosensing Platforms

Signal Amplification Strategy Design in Nanozyme‐Based Biosensors for Highly Sensitive Detection of Trace Biomarkers

Nanomaterials for Molecular Detection and Analysis of Extracellular Vesicles

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