FE-SEM Evaluation of Dental Specimens Prepared by Different Methods for<i>In Vitro</i>Contamination

Nakamura, Vitor Cesar; Kataoka, Simony Hidée Hamoy; Gavini, Giulio; Ferrari, Patricia Helena Pereira; Cai, Silvana

doi:10.1155/2012/748471

Cited by 4 publications

(4 citation statements)

References 19 publications

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“…The mesiobuccal canals were enlarged up to a #20 K file to ensure space for posterior bacterial contamination. The teeth were treated with 17% ethylenediaminetetraacetic acid (EDTA; Merck, Darmstadt, Germany) for 5 min in an ultrasonic bath to remove the smear layer [ 21 ]. The outer surfaces and apexes of the root canals were coated with nail varnish to create a closed-end system.…”

Section: Methodsmentioning

confidence: 99%

Effectiveness of conservative instrumentation in root canal disinfection

et al. 2023

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Objectives The impact of conservative instrumentation on the disinfection of root canals with different curvatures has not yet been determined. This ex vivo study aimed to evaluate and compare the effect of conservative instrumentation with TruNatomy (TN) and Rotate and a conventional rotary system, ProTaper Gold (PTG), on root canal disinfection during chemomechanical preparation of straight and curved canals. Materials and methods Ninety mandibular molars with straight (n = 45) and curved (n = 45) mesiobuccal root canals were contaminated with polymicrobial clinical samples. Teeth were divided into three subgroups (n = 14) according to the file systems and the curvature. Canals were instrumented with TN, Rotate, and PTG, respectively. Sodium hypochlorite and EDTA were used as irrigants. Intracanal samples were taken before (S1) and after (S2) instrumentation. Six uninfected teeth were used as negative controls. The bacterial reduction between S1 and S2 was measured by ATP assay, flow cytometry, and culture methods. Kruskal–Wallis and ANOVA tests were followed by the Duncan post hoc test (p < 0.05). Results Bacterial reduction percentages were similar for the three file systems in straight canals (p > 0.05). However, PTG showed a lower reduction percentage of intact membrane cells in flow cytometry than TN and Rotate (p = 0.036). For the curved canals, no significant differences were obtained (p > 0.05). Conclusion Conservative instrumentation of straight and curved canals using TN and Rotate files resulted in similar bacterial reduction compared to PTG. Clinical relevance The disinfection efficacy of conservative instrumentation is similar to conventional instrumentation in straight and curved root canals.

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Section: Methodsmentioning

confidence: 99%

Effectiveness of conservative instrumentation in root canal disinfection

et al. 2023

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“…The teeth were cleaned using ultrasonic scaler followed by sterilization under autoclave as suggested in previous studies. [ 18 19 ] Thereafter, teeth were stored in distilled water until use. The teeth were decoronated below the cementoenamel junction using the diamond disc to obtain standardized root length of 10 mm.…”

Section: Methodsmentioning

confidence: 99%

Smear layer removal efficacy of combination of herbal extracts in two different ratios either alone or supplemented with sonic agitation: An in vitro scanning electron microscope study

2015

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Objectives:The study aimed to evaluate the effectiveness of the combination of two natural extracts in varying ratios for removal of smear layer either alone or supplemented with sonic agitation.Materials and Methods:Fifty extracted single-rooted teeth were collected, disinfected and decoronated below the cementoenamel junction to obtain standardized root length of 10 mm. Root canals were instrumented using rotary files at working length 1 mm short of the apex. Specimens were divided into six groups according to the irrigation protocol as follows: Group A – Distilled water, Group B – 17% ethylenediaminetetraacetic acid, Group C – Herbal extracts in 1:1 ratio, Group D – Herbal extracts in 1:1 ratio supplemented with sonic agitation, Group E – Herbal extracts in 2:1 ratio, Group F – Herbal extracts in 2:1 ratio supplemented with sonic agitation. Specimens were longitudinally sectioned and evaluated under scanning electron microscope for smear layer removal efficacy. Obtained scores were statistically analyzed using one-way analysis of variance and post-hoc test.Results:Among all, Group B showed the best results followed by Group F. Remaining other groups showed inferior outcome (P < 0.05).Conclusion:The combination of two extracts in 2:1 ratio was slightly better than 1:1 ratio and the smear layer removal efficacy was further improved when accompanied with sonic agitation.

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“…Afterwards, three randomly selected specimens from negative (demineralized untreated and unrestored) and positive (sound and protected) control dentin specimen and restored groups were further sectioned bucco-lingually into very thin ground sections of 0.33 to 0.4 mm in thickness using the Isomet microsaw. An optical microscope (Olympus Corporation, Tokyo, Japan) connected to digital camera (TUCSEN 2.0 MP USB microscope digital camera, China) was used to examine areas of sound, demineralized and remineralized dentin of those teeth ground sections (18).…”

Section: X-ray Spectroscopy (Edx) and Optical Microscopymentioning

confidence: 99%

Effect of Using Biomimetic Analogs on Dentin Remineralization with Bioactive Cements

2020

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This study evaluated the impact of using biomimetic analogs (poly-acrylic acid and sodium tri-meta-phosphate) on dentin remineralization using two cement materials, the first is calcium silicate based and the second is calcium hydroxide based materials. Two standardized occlusal cavities (mesial and distal) were prepared within dentin after removal of occlusal enamel. Artificial demineralized dentin was induced through pH cycling (8 h in demineralizing and 16 h in remineralizing solutions). Demineralized cavities were divided into four groups; two groups received cement materials. The other groups were first treated with biomimetic analogs then restored with pulp cement materials. Teeth were sectioned buccolingually into two halves. Treated cavities with analogs were stored in simulated body fluid containing poly-acrylic acid. Untreated cavities were stored in simulated body fluid only. Ground unstained sections of demineralized dentin were examined using light microscope. Specimens were examined after 1, 6 and 12 weeks of storage using energy dispersive X-ray Spectroscopy (EDX) and Vickers microhardness was evaluated. Two-way ANOVA was used to analyze data statistically. Calcium silicate-based cement group with biomimetic analogs showed the highest statistically significant calcium and phosphorous wt% in addition to highest surface hardness values after 12 weeks of storage. Demineralized dentin ground sections showed increase in light zones after total period of storage. Calcium silicate-based cement showed the best ability to enrich the artificial carious dentin with ions for remineralization. Using biomimetic analogs had a significant impact on demineralized dentin surface hardness improvement.

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FE-SEM Evaluation of Dental Specimens Prepared by Different Methods forIn VitroContamination

Cited by 4 publications

References 19 publications

Effectiveness of conservative instrumentation in root canal disinfection

Effectiveness of conservative instrumentation in root canal disinfection

Smear layer removal efficacy of combination of herbal extracts in two different ratios either alone or supplemented with sonic agitation: An in vitro scanning electron microscope study

Effect of Using Biomimetic Analogs on Dentin Remineralization with Bioactive Cements

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