Fe–N–C Single-Atom Nanozymes for the Intracellular Hydrogen Peroxide Detection

Abstract

Recently, in situ detection of hydrogen peroxide (H 2 O 2 ) generated from live cells have caused tremendous attention, because it is of great significance in the control of multiple biological processes. Herein, Fe−N−C single-atom nanozymes (Fe−N−C SAzymes) with intrinsic peroxidase-like activity were successfully prepared via high-temperature calcination using FeCl 2 , glucose, and dicyandiamide as precursors. The Fe−N−C SAzymes with FeN x as active sites were similar to natural metalloproteases, which can specifically enhance the peroxidase-like activity rather than oxidase-like activity. Accordingly, owing to the excellent catalytic efficiency of the Fe−N−C SAzymes, colorimetric biosensing of H 2 O 2 in vitro was performed via a typical 3,3′,5,5′-tetramethylbenzidine induced an allochroic reaction, demonstrating the satisfactory specificity and sensitivity. With regard to the practical application, in situ detection of H 2 O 2 generated from the Hela cells by the Fe−N−C SAzymes was also performed, which can expand the applications of the newborn SAzymes.

The Fe‐N‐C Nanozyme with Both Accelerated and Inhibited Biocatalytic Activities Capable of Accessing Drug–Drug Interactions

“…Such features offer a great opportunity to mimic more advanced behaviors of CYP using Fe‐N‐C. Nonetheless, the prior synthesis of Fe‐N‐C nanozyme mostly adopted the same strategies for ORR electrocatalysts, making Fe‐N‐C far less than fully optimized as a nanozyme.…”

Hydrogen Peroxide Sensors for Biomedical Applications

“…Additionally in 2019, Jiao et al detected intracellular H 2 O 2 in cervical cancer cells (HeLa) by using iron-nitrogen-carbon single-atom nanozymes, which exhibit peroxidase-like activity, allowing for the catalyzation of H 2 O 2 [37].…”

“…H2O2 sensors that could be used by researchers, including those outlined below [10,25,[28][29][30][31][32][33][34][35][36][37][38]. light that can then be read to determine H2O2 concentration [16].…”

Interactions of Fe–N–S Co-Doped Porous Carbons with Bacteria: Sorption Effect and Enzyme-Like Properties

“…ROS may be generated abiotically when hydrogen peroxide reacts with Fe-containing nanomaterials leading to the production of superoxide radicals ( • O 2 − ), hydroxyl radicals ( • OH), or singlet oxygen species ( 1 O 2 ). This process is similar to the activity of peroxidases, which catalyze the reaction of H 2 O 2 with organic substrates via ROS generation [3][4][5][6]. For these reasons, the possible antibiotic properties of the nanozymes as well as their implementation for nanotechnology as the ROS determination agents have been studied intensively over the last several years.…”