Fe(II)-Co(II) Double Salt Incorporated Magnetic Hydrophobic Microparticles for Invertase Adsorption

Abstract

Invertase (β-fructofuranoside fructohydrolase, EC 3.2.1.26) is an enzyme widely used in the food industry. Its main function is the formation of glucose and fructose through hydrolysis of sucrose. For the separation and purification of this commercially important enzyme from aqueous solutions, magnetic poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-tryptophan), m-poly(HEMA-MATrp) microparticles were developed. Magnetic properties of microparticles are provided using ferromagnetic Fe(II)-Co(II) double salt. Characterization studies of magnetic microparticles were conducted via vibrating sample magnetometer (VSM), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) analysis. Specific surface area of magnetic microparticles is 6.75 m(2)/g. Because of all experiments performed in this study, the adsorption capability of magnetic microparticles was optimized by variation of different conditions (pH, interaction time, initial invertase concentration, temperature, and ionic strength) and maximum adsorption capacity was determined as 992.64 mg invertase/g magnetic microparticles.

Nicotinamide-Modified poly(HEMA-GMA)-Nic Cryogels for Removal of Pesticides

“…As can be seen from Figure 3B, the synthesized cryogels have cavities that allow the analyte to diffuse readily. The presence of holes in the porous structure, having a water retention capacity of about ~800%, although having a surface area of ~8.5 m 2 /g less than microparticles or nanoparticles (28)(29)(30)(31)(32)39), provides the necessary retention time for the analyte to interact with ligand within polymeric structure. Ability to swallow high amount of analytes and the cavities connected with interconnected flow channels are the critical properties of cryogels providing them to be indispensable absorbents.…”

“…In particular, the micro-and macroporous structure of cryogels overcome the disadvantages in conventional techniques. The primary downside of cryogels over micro-and nano-adsorbents is that they seem to have a low surface area, but they also eliminate many problems coming from these adsorbents such as agglomeration, low yield, and the need for highperformance devices such as centrifuges (28)(29)(30)(31)(32). Polymeric cryogels have interconnected flowchannels providing the diffusion of the target molecule to the cavities of the cryogels, therefore, this provides a healthy interaction between the ligands on the surface of cryogels and the target molecules residing on the cavities for a while (33).…”

Efficient polymeric material for separation of human hemoglobin

“…The synthesis of net-poly(HEMA-co-AA) microparticles was performed according to the literature (Erol et al 2015) without using any magnetic core through microemulsion polymerization techniques using structural monomer (HEMA, 2 mL) and crosslinker (NNMBA, 3.27 g), functional monomer (AA, 500 lL), toluene (6 mL), PVA (200 mg) and distilled water of 50 mL. At the last stage, the azobisisobutyronitrile (AIBN) was added as an initiator.…”

Endosulfan Elimination Using Amine-Modified Magnetic Diatomite as an Adsorbent

“…Applications of magnetic separation techniques using magnetic particles and their improvement have sparked researchers' interest in recent years (Köse et al, 2015;Erol et al, 2016). Using natural materials with magnetic properties provides several advantages in terms of specific affinity, better selectivity, more simplicity, and higher adsorption rates.…”