Efficient polymeric material for separation of human hemoglobin

Abstract

In this study, negatively charged acrylic acid was used as a functional ligand to synthesise net-poly(2-hydroxyethyl methacrylate-co-acrylic acid) microparticles for the removal of hemoglobin having a positively charged Feion in the core, as an alternative to conventional techniques to achieve a cost effective high-capacity purification. The characterization of microparticles was performed via Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, atomic force microscopy, the Brunauer - Emmett - Teller surface area analysis, and swelling test methods. Optimum adsorption conditions such as pH, initial hemoglobin concentration, temperature, and interaction time were studied batch wise. The highest adsorption capacity of microparticles was observed at pH 7.4 as 1276.4 mg hemoglobin/g polymer. The reusability of microparticles was also promising with ∼5% decrease in the adsorption capacity at the end of the five adsorption-desorption cycles.

Facile and green preparation of multifeatured montmorillonite-supported Fe3O4-Cu2+ hybrid magnetic nanomaterials for the selective adsorption of a high-abundance protein from complex biological matrices

“…4 Among various removal technologies, adsorption is the preferred tool due to its facile operation, low cost, and inability to cause secondary pollution. Although many kinds of adsorbents have been developed [5][6][7][8] in the past to isolate hemoglobin from complicated biological samples, most of them exhibit several drawbacks such as non-eco-friendly syntheses, low adsorption capacities, poor stabilities, high costs, or tedious separation processes, all of which greatly impede their practical utilization. Thus, it is imperative to explore novel, smart, and green adsorbents with satisfactory adsorption performance towards hemoglobin.…”