Fcγ receptor polymorphisms in populations in Ethiopia and Norway

Abstract

SUMMARYSeventy-seven healthy Ethiopians were genotyped for polymorphisms in the immunoglobulin G Fc receptors (FccR) FccRIIa, FccRIIIa and FccRIIIb, including the SH allele. The genotype and allele frequencies were compared with those of 96 healthy Norwegians. Ethiopians had higher frequencies of the SH-FccRIIIb (P=0 . 001), FccRIIIa-158 V (P=0 . 026) and FccRIIIb-Na2 (P=0 . 046) alleles. The genotype distributions of FccRIIa, FccRIIIa and FccRIIIb, however, did not differ signi®cantly from those of the Norwegians. The data were also compared with those reported from studies on other ethnic groups. The variation of different polymorphisms both within and between ethnic groups may in¯uence differences in the incidence rates of infectious and autoimmune diseases.

Rituximab in Severe Lupus Nephritis

“…In our study, Fc␥RIIIa genotyping was not available. However, the Fc␥RIIIa-158F allele, possibly associated to B cell depletion failure, does not seem to be more frequent in African than in Caucasian population (26), suggesting other unrecognized black ethnicity-associated B cell resistance factors.…”

Relationship Between Fcγ Receptor and Interleukin-1 Gene Polymorphisms and Post-treatment Apical Periodontitis

“…To ascertain the availability of human DNA for analysis, aliquots of 5 mL of the DNA extracts from clinical samples were subjected to PCR by using primers directed to the T-cell receptor Va22 gene (V22f:5 0 -GAT TCA GTG ACC CAG ATG GAA GGG-3 0 and V22r:5 0 -AGC ACA GAA GTA CAC CGC TGA GTC-3 0 ), which amplify a fragment of 270 bp (35). PCR amplifications were performed in 50 mL of reaction mixture containing 5 mL of 10 Â PCR buffer (Fermentas), 1 mmol/L concentration of each primer, 1.25 U of Taq DNA polymerase (Fermentas), 3 mmol/L MgCl 2 , and 0.2 mmol/L of each deoxyribonucleoside triphosphate (Biotools).…”

Humoral Immune Response to Salmonella Antigens and Polymorphisms in Receptors for the Fc of IgG in Patients with Ankylosing Spondylitis

“…In order to determine the FcγRIIIa genotypes, two reactions were carried out for each sample, according to the method described by Van Der Berg et al, (Van Den Berg et al, 2001). PCRs were performed by adding, 100 ng of genomic DNA, 200 µM of each dNTPs, 6 mM MgCl 2 , 20 ng of each control primer (Ctrl-1 and Ctrl-2), 200 ng of KIM-G(V) or KIM1(F) primer in their respective reaction, and 2.0 U of Taq polymerase (Invitrogen) to a 50 µL solution containing PCR buffer 1X (Invitrogen).…”