Fauna flebotomínica (Diptera: Psychodidae) da Serra do Tepequém, Município de Amajarí, Estado de Roraima, Brasil

Neto, Jaime de Liege Gama; Freitas, Rui Alves de; Baima, Janderson Melo; Passos, Mahedy Araujo Bastos

doi:10.5123/s2176-62232010000200016

Cited by 3 publications

(2 citation statements)

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“…Regarding E. georgii, its type locality is in the municipality of Oriximiná, state of Pará (RYAN, 1986), but this species was described only in 2002 (FREITAS & BARRETT, 2002) based on specimens that had previously been identified as Evandromyia begonae (Ortiz & Torrez), and because of errors in identification, it may be that its real distribution has been underestimated. This species has already been found in Roraima, Amazonas and Pará, which are all states in the Brazilian Amazon region (FREITAS & BARRETT, 2002;GAMA et al, 2010;RYAN, 1986). The finding of these two species increases the number of sandflies registered in Rondônia: recently, Galardo et al (2015) elevated the number of species in Rondônia to 118 and, with our findings, this number is updated to 120 species.…”

Section: Discussionmentioning

confidence: 88%

Sandfly fauna (Diptera: Psychodidae) from caves in the state of Rondônia, Brazil

Ogawa

Júnior

Resadore

et al. 2016

Rev. Bras. Parasitol. Vet.

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This study had the aim of ascertaining the sandfly fauna and possible presence of Leishmania in these insects, collected in caves in the state of Rondônia, Brazil. Collections were conducted in eight caves located in two different areas of this state. Leishmania in the sandflies collected was detected using the polymerase chain reaction (PCR). This was the first study on sandflies from caves in Rondônia and, among the total of 1,236 individuals collected, 24 species and 10 genera were identified. The species Evandromyia georgii was collected for the first time in Rondônia and the most abundant species were Trichophoromyia ubiquitalis with 448 individuals (36.2%), followed by T. octavioi with 283 (22.9%) and E. georgii with 179 (14.5%). For the PCR, 17 pools were analyzed and five pools were positive (for T. auraensis in three pools and for Nyssomyia shawi and N. antunesi in one pool each). The kDNA region was amplified and the presence of Leishmania DNA was confirmed. The sandfly fauna in these caves can be considered diverse in comparison with similar studies in other regions. It may be that some species use caves as a temporary shelter and breeding site, while other species live exclusively in this environment. The detection of Leishmania DNA indicates that this pathogen is circulating in cave environments and that further studies are needed in order to ascertain the risks of infection by leishmaniasis in these locations with high touristic potential.Keywords: Caves, sandflies, Rondônia, Amazon. ResumoO objetivo deste estudo foi conhecer a fauna de flebotomíneos, e possível presença de Leishmania nestes insetos, coletados em cavernas do Estado de Rondônia. As coletas foram realizadas em oito cavernas localizadas em duas áreas diferentes do Estado. A detecção de Leishmania nos flebotomíneos foi realizada por reação em cadeia da polimerase (PCR). Este foi o primeiro trabalho com flebotomíneos em cavernas de Rondônia e um total de 1,236 indivíduos foram coletados e identificados em 24 espécies e 10 gêneros. Evandromyia georgii foi coletada pela primeira vez em Rondônia, e as espécies mais abundantes foram Trichophoromyia ubiquitalis com 448 indivíduos (36.2%) seguida por T. octavioi com 283 (22.9%) e E. georgii com 179 (14.5%). No estudo de PCR, 17 pools foram analisados, sendo cinco positivos (T. auraensis -3, Nyssomyia shawi e N. antunesi -1 cada). A região do kDNA foi amplificada confirmando a presença de DNA de Leishmania. A fauna de flebotomíneos nestas cavernas foi considerada diversa quando comparada com estudos semelhantes de outras regiões. É possível que algumas espécies utilizem cavernas como abrigo temporário e local de procriação e outras sejam exclusivas deste ambiente. A detecção de DNA de Leishmania indica que este patógeno está circulando no ambiente cavernícola, sendo necessários mais estudos para conhecer o risco de transmissão de leishmanioses nestes locais com alto potencial turístico.Palavras-chave: Cavernas, flebotomíneos, Rondônia, Amazônia.

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Section: Discussionmentioning

confidence: 88%

Sandfly fauna (Diptera: Psychodidae) from caves in the state of Rondônia, Brazil

Ogawa

Júnior

Resadore

et al. 2016

Rev. Bras. Parasitol. Vet.

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“…This scenario poses challenges to implement measures recommended by the Brazilian Ministry of Health for the control of CL and is further complicated by the multiple ethnic, cultural and environmental factors that characterize this region [ 4 , 5 ]. Previous studies have shown that Brazil’s Amazon region has undergone profound environmental changes resulting from mining, agriculture and livestock expansion [ 6 ], which may be contributing to changes and maintenance of the leishmaniasis cycle in the area. The State of Roraima, which is in this region, has experienced these changes with an increase of leishmaniasis cases between 2011 to 2015 [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Diagnosis and identification of Leishmania species in patients with cutaneous leishmaniasis in the state of Roraima, Brazil's Amazon Region

et al. 2021

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Background Cutaneous leishmaniasis (CL) is an endemic disease in Brazil that is highly prevalent in the northern region of the country. Although there is a continuous and growing number of cases registered in the state of Roraima, there is limited information regarding the species of Leishmania that affect the human population. In this study, we aimed to characterize which Leishmania species cause human disease in those presenting with cutaneous leishmaniasis in endemic areas of the State of Roraima. Methods We conducted a prospective surveillance study between 2016 to 2018 in health centers located in the State of Roraima, Brazil. Participants with clinical suspicion of CL were enrolled and provided lesion samples for parasitological detection by microscopy. A subset of the samples was tested by polymerase chain reaction and sequencing of the internal transcribed spacer 1 (ITS-1 PCR) for molecular species identification. Results A total of 262 participants were enrolled in this study. Of those, 129 (49.27%) were positive by parasitological examination. Most positive subjects (81.58%) were male, and most cases presented a single lesion (80.26%). ITS-1 PCR and sequencing on a subset of 76 samples allowed us to detect nine different species of Leishmania: L. (V.) braziliensis, L (V.) panamensis, L. (V.) guyanensis, L. (V.) naiffi, L. (V.) shawi, L.(V.) utingensis, L. (V.) lindenbergi, L. (L.) amazonensis and L. (L.) mexicana. Conclusions Our study provides the first assessment of circulating species of Leishmania in the State of Roraima, Brazil, and shows the high diversity in this region. This study opens the path for further research on the transmission of leishmaniasis in the northernmost Brazilian State including vector and reservoir surveillance as well as for intensification of investigation and control activities against CL in the region.

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Identification of Bloodmeals from Sand Flies (Diptera: Psychodidae) Collected in the Parque Nacional do Viruá, State of Roraima, Brazil

Rodrigues¹,

Costa

Shimabukuro

2021

Journal of Medical Entomology

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The transmission of pathogens that cause leishmaniases occurs by the bite of female sand flies (Diptera: Psychodidae) in their vertebrate hosts, which makes the identification of their bloodmeal sources an important step for the control and epidemiology of these diseases. In Brazil, the state of Roraima has a great diversity of sand flies, vertebrate hosts, and protozoan Leishmania, but little is known about the host blood-feeding preferences of sand flies. Thus, we evaluated the bloodmeal sources of sand flies collected from their sylvatic habitats in Parque Nacional do Viruá, Roraima. Fieldwork was carried-out between 13th and 18th August 2019 using CDC light traps. Sand flies were slide-mounted and morphologically identified using the head and last segments of the abdomen. Engorged females had their DNA extracted, followed by amplification and sequencing of the cytochrome b (cytb) molecular marker for vertebrates. Sequences were analyzed and compared with those from GenBank using the BLASTn search tool, in addition to the reconstruction of a phylogenetic tree to demonstrate the clustering pattern of these sequences. A total of 1,209 sand flies were identified, comprising 20 species, in which the most abundant were Psychodopygus ayrozai (Barretto and Coutinho) (42.10%) and Psychodopygus chagasi (Costa Lima) (26.22%). Bloodmeal source identification was successfully performed for 34 sand flies, that confirm four vertebrate species, being the most abundant the armadillo Dasypus novemcinctus Linnaeus, 1758 (Cingulata: Dasypodidae).

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Fauna flebotomínica (Diptera: Psychodidae) da Serra do Tepequém, Município de Amajarí, Estado de Roraima, Brasil

Cited by 3 publications

References 2 publications

Sandfly fauna (Diptera: Psychodidae) from caves in the state of Rondônia, Brazil

Sandfly fauna (Diptera: Psychodidae) from caves in the state of Rondônia, Brazil

Diagnosis and identification of Leishmania species in patients with cutaneous leishmaniasis in the state of Roraima, Brazil's Amazon Region

Identification of Bloodmeals from Sand Flies (Diptera: Psychodidae) Collected in the Parque Nacional do Viruá, State of Roraima, Brazil

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