Fatty Infiltration of the Torn Rotator Cuff Worsens Over Time in a Rabbit Model

Rubino, L. Joseph; Stills, Harold F.; Sprott, D.; Crosby, Lynn A.

doi:10.1016/j.arthro.2007.01.023

Cited by 88 publications

(93 citation statements)

References 11 publications

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“…Many animal studies have shown that fatty degeneration in muscles with rotator cuff tears is localized in the musculotendinous junction, which is in the distal portion of the muscle. 24,26 Our result was consistent with these previous reports. The musclo-tendinous junction might be abundant in the pluripotent stem cells.…”

Section: Discussionsupporting

confidence: 83%

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Molecular mechanism of fatty degeneration in rotator cuff muscle with tendon rupture

Itoigawa

Kishimoto

Sano

et al. 2011

Journal Orthopaedic Research

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Fatty degeneration often occurs in rotator cuff muscle with tendon rupture. However, the molecular mechanism underlying this change has not been fully clarified yet. We investigated the gene expression of Wnt10b and adipogenic marker gene, PPARg and C/EBPa in C2C12 myogenic cell line under inhibition of Wnt10b by adipogenic induction medium, isobutylmethylxanthine, dexamethasone, and insulin (MDI). The role of Wnt-signal was confirmed by adding Lithium chloride (LiCl), which mimics Wnt signaling to the cultured cell with MDI. We also assessed the expression profiles of same genes in the rat rotator cuff tear model in vivo. MDI induced Oil red-O staining positive adipocytes and upregulated PPARg and C/EBPa expression. LiCl inhibited adipogenic induction of MDI. Rotator cuff muscle with tendon rupture showed positive staining for Oil red-O. Real-time polymerase chain reaction analyses revealed decreased expression of Wnt10b followed by increased PPARg and C/EBPa gene expression in the supraspinatus muscle. Fatty degeneration and its molecular events were remarkably seen in the distal one-third of the detached supraspinatus muscle versus control. Wnt signaling may regulate adipogenic differentiation both in the myoblasts in vitro and the muscle in vivo. Our results indicate that the reduction of Wnt10b in muscle with a rotator cuff tear is a key signal in fatty degeneration of the muscle. ß

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Section: Discussionsupporting

confidence: 83%

“…6,24,25 The term ''infiltration'' conveys a sense of permeation of lipids into muscle. As we showed in this in vitro study, myoblasts can trans-differentiate to PPARg expressing adipocytes.…”

Section: Discussionmentioning

confidence: 99%

Molecular mechanism of fatty degeneration in rotator cuff muscle with tendon rupture

Itoigawa

Kishimoto

Sano

et al. 2011

Journal Orthopaedic Research

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“…Furthermore, the sample was harvested from the distal third of the lateral portion of the muscle, which is close to the myotendinous junction. This area was previously described as having the highest degree of fat accumulation in rat31 and rabbit32, 33 models of RC tears. Due to potential regional differences, the calculated volumes of contractile, fatty, and other types of tissue should be considered as approximate values.…”

Section: Discussionmentioning

confidence: 95%

Costamere protein expression and tissue composition of rotator cuff muscle after tendon release in sheep

Ruoss

Möhl

Benn

et al. 2017

Journal Orthopaedic Research

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Previous studies suggested that degradation of contractile tissue requires cleavage of the costamere, a structural protein complex that holds sarcomeres in place. This study examined if costamere turnover is affected by a rotator cuff tear in a previously established ovine model. We found the activity of focal adhesion kinase (FAK), a main regulator of costamere turnover, was unchanged at 2 weeks but decreased by 27% 16 weeks after surgical release of the infraspinatus tendon. This was accompanied by cleavage of the costamere protein talin into a 190 kDa fragment while full length talin remained unchanged. At 2 weeks after tendon release, muscle volume decreased by 17 cm3 from an initial 185 cm3, the fatty tissue volume was halved, and the contractile tissue volume remained unchanged. After 16 weeks, the muscle volume decreased by 36 cm3, contractile tissue was quantitatively lost, and the fat content increased by 184%. Nandrolone administration mitigated the loss of contractile tissue by 26% and prevented fat accumulation, alterations in FAK activity, and talin cleavage. Taken together, these findings imply that muscle remodeling after tendon release occurs in two stages. The early decrease of muscle volume is associated with reduction of fat; while, the second stage is characterized by substantial loss of contractile tissue accompanied by massive fat accumulation. Regulation of costamere turnover is associated with the loss of contractile tissue and seems to be impacted by nandrolone treatment. Clinically, the costamere may represent a potential intervention target to mitigate muscle loss after a rotator cuff tear. © 2017 The Authors. Journal of Orthopaedic Research® published by Wiley Periodicals, Inc. on behalf of the Orthopaedic Research Society. J Orthop Res 36:272–281, 2018.

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“…Relatively distally located evaluation points may not well represent the condition of the entire muscle. Rotator cuff tear with fatty infiltration is a progressive and infiltrative process that increases with time and fatty infiltration progresses from the musculotendinous junction toward the muscle origin in a rabbit torn cuff model [19]. The FD of the cuff might be overestimated when the evaluation is performed on one cut section near the glenoid.…”

Section: Discussionmentioning

confidence: 99%

Reliability of the Grading System for Fatty Degeneration of Rotator Cuff Muscles

Kim

Choi

et al. 2010

Clinical Orthopaedics &Amp; Related Research

158

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Fatty degeneration of the rotator cuff muscles is considered one of the most important factors for the outcomes of cuff repair. However, the reliability of the grading system is not well validated. Two specialists in musculoskeletal radiology and three shoulder fellowshiptrained orthopaedic surgeons reviewed the fatty degeneration grades of each cuff muscle of consecutive 75 fullthickness cuff tears. Fatty degeneration grades were assessed according to the systems of Goutallier et al. and Fuchs et al. using preoperative MR and postoperative CT arthrographies. The interclass correlation coefficient was analyzed to assess interobserver and intraobserver reliabilities. For interobserver reliability using the system of Goutallier et al. the interclass correlation coefficient was higher in MR arthrography (0.6-0.72) than in CT arthrography (0.43-0.6) and higher for radiologists (0.58-0.78) than for orthopaedic surgeons (0.32-0.68).

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Fatty Infiltration of the Torn Rotator Cuff Worsens Over Time in a Rabbit Model

Cited by 88 publications

References 11 publications

Molecular mechanism of fatty degeneration in rotator cuff muscle with tendon rupture

Molecular mechanism of fatty degeneration in rotator cuff muscle with tendon rupture

Costamere protein expression and tissue composition of rotator cuff muscle after tendon release in sheep

Reliability of the Grading System for Fatty Degeneration of Rotator Cuff Muscles

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