Fatty Acids Rich Extract From Clerodendrum volubile Suppresses Cell Migration; Abates Oxidative Stress; and Regulates Cell Cycle Progression in Glioblastoma Multiforme (U87 MG) Cells

Abstract

Glioblastoma multiforme (GBM) is a malignant primary type of brain cancer with high proliferation and metastasis rates due to involvement of the microglial cell. It is resistant against available chemotherapy. Many strategic protocols have been developed but prognosis and patient life has not improved substantially. In this study, the anti-metastatic and antioxidant effect of fatty acids from Clerodendrum volubile leaves were investigated in U87-MG (Human Glioblastoma Multiforme) cell lines. The extracted fatty acids were incubated with U87-MG cells for 48 h. The anti-proliferative effect was determined by MTT assay, while apoptosis and cell cycle were analyzed with BD FACSCalibur. The transwell assay protocol was utilized in the analysis of cell migration and invasion. The treated cell lines were also assessed for reduced glutathione (GSH) level, catalase, superoxide dismutase (SOD) and lipid peroxidation. The fatty acid extract showed significant inhibitory activity on cell proliferation and cell cycle progression, mitigated oxidative stress, and suppressed migration and invasion in U-87 MG cell lines. These results give credence to the therapeutic potential of this plant against cancer, especially GBM.

Tetrahydrocannabinol-Rich Extracts From Cannabis Sativa L. Improve Glucose Consumption and Modulate Metabolic Complications Linked to Neurodegenerative Diseases in Isolated Rat Brains

“…Studies have reported therapeutic failures in patients owing to difficulty in treatment (Puli et al, 2006). Although medicinal plants have reported to arrest the proliferation of GBM (Erukainure et al, 2018), C. sativa extracts showed no cytotoxic effect on the cells (Figure 7). Thus, insinuating that the plant may not be beneficial in the treatment of GBM.…”

“…Glioblastoma multiforme (GBM) is a malignant primary brain tumor common in young kids ( Erukainure et al, 2018 ). Studies have reported therapeutic failures in patients owing to difficulty in treatment ( Puli et al, 2006 ).…”

Anti-migration and Anti-invasion Abilities of Methanolic Leaves Extract of Clerodendrum Inerme on Lung Cancer Cells

“…34 Previous reports demonstrated that fatty acids extracted from C. volubile leaves could suppress tumor metastasis and invasion in human neuronal glioblastoma cells. 35 and breast cancer cells. 36 Although the mechanism underlying antimigration, anti-invasion and anti-adhesion effects are still unclear, the result demonstrated that C. inerme methanolic leaves extract could suppress cancer metastasis of lung cancer cell lines.…”

Targeting the initiation and termination codons of SARS-CoV-2 spike protein as possible therapy against COVID-19: the role of novel harpagide 5-O-β-D-glucopyranoside from Clerodendrum volubile P Beauv. (Labiatae)

“…The dichloromethane (DCM) fraction of the leaves have been reported for its cytotoxic effect on human embryonic kidney (HEK293) cells (Erukainure, Narainpersad, et al, 2018 ). The leaves are rich in phenolics contents which has been correlated with its reported biological activities (Erukainure, Narainpersad, et al, 2018 ; Oboh et al, 2017 ).…”

“…Regarded as magic leaves, C. volubile is used in folk medicine for treating ulcers, arthritis, rheumatism, dropsy, and diabetes (Burkill, 1985 ). Its antioxidant activities have been reported in diabetic rats, and cancerous cells which correlates with their antidiabetic and anticancer activities (Erukainure, Ashraf, et al, 2018; Erukainure, Hafizur, et al, 2017 ; 2018 ). The flowers as well as protocatechuic acid and pectolinarigenin isolated from the leaves have been reported for their ability to modulate phagocytic oxidative burst in isolated neutrophils and macrophages which demonstrates their immunomodulatory activities (Erukainure, Hafizur, et al, 2017 ; Erukainure, Mesaik, et al, 2017 ; Erukainure et al, 2016 ).…”