Fatty Acids Composition of Microbacterium Genus Bacteria – Destructors of Oil Hydrocarbons

Abstract

To conduct an identification of microorganisms -destructors of oil hydrocarbons -based on their fatty acid composition. This is one of the chemotaxonomic characteristics that correlate with bacterial molecular genetic features. Fatty acids analysis of cells of bacteria was carried out using automatic system of microorganisms' identification MIDI Sherlock (MIDI, USA) based on gas chromatograph Agilent 7890. For fatty acids profiles test culture were characterized by С 15 :

Fatty acids composition of Bacillus subtilis ONU551 lipids

“…The total content of saturated and unsaturated fatty acids of the branched structure in the form -iso: reached 47% (14:0 iso, 0.52%; 15:0 iso, 34.72%; 16:0 iso, 1.85%; 17:1 iso w10c, 3.18%; 17:0 iso, 7.11%) and very slightly prevailed over the total content of branched fatty acids in the form -anteiso (∑w = 44%): 15:0 anteiso (33.72%), 17:0 anteiso (10.24%), that is, they were almost equal. For comparison, it can be noted that in the fatty acid profile of bacteria belonging to another genus, for example, Microbacterium barkeri OZ-3 and Microbacterium barkeri OZ-2-destructors of oil and petroleum products , unlike the strain B. subtilis ONU551 studied by us, saturated fatty acids prevailed branched structure in the form of -аnteiso: (∑w = 72.3-73.4%) 12-methyltetradecanoic (15:0 аnteiso) and 14-methylhexadecanoic (17:0 anteiso) acids [16].…”

Sinomonas terrae sp. nov., Isolated from an Agricultural Soil

“…The strain 5-5 T , Sinomonas humi NBRC 110653 T , Sinomonas susongensis JCM 31752 T , Sinomonas atrocyanea KACC 17386 T , and Sinomonas soli KCTC 19389 T were cultivated on R2A agar at 30°C for 2 days at the same time. After cultivation, the cellular fatty acids were extracted, methylated, and separated using gas chromatography (model 7890; MIDI, USA) following the Sherlock Microbial Identification System's protocol [ 23 ]. The identification and quantification of fatty acid methyl esters were performed using the Sherlock Microbial Identification System's TSBA 6 database (version 6.3).…”