Oil extracted from the wild plant of Cistanche phelypaea was analyzed for its fatty acid, sterol, hydrocarbon and tocopherol contents. Total lipids (TL) content was 10 g/kg (on dry weight basis). The majority of fatty acids were of the unsaturated type (50.4 % of total fatty acids), while the saturated (mainly palmitic acid) were about 43.2 % of the total fatty acids. Oleic acid was the dominating fatty acid followed by palmitic and linoleic acids. High amounts of sterols were found in the oil with the main component b-sitosterol. Other phytosterols (e.g. stigmasterol, D 7 -avenasterol and D 5 -avenasterol) were present at approximately equal amounts (6-9 % of total sterols). The main identified hydrocarbon compounds were C 21 , C 26 and C 32 constituting about 61.2 % of total hydrocarbons. Small amounts of C 12 , C 18 and C 22 , however, were also detected. Tocopherol levels were high in the oil (3.36 g/kg oil), wherein b-tocopherol was the main component followed by a-isomer. Both tocopherol components comprised more than 87 % of total vitamin E content in the oil. Furthermore, c-and d-tocopherols were detected in small amounts in the oil accounting for 14-16 % of the total vitamin E content. Information provided by the present work will be of importance for food applications and chemotaxonomy of Cistanche phelypaea.