Characterization of fatty acids, bioactive lipids, and radical scavenging activity of Canterbury bells seed oil

Ramadan, Mohamed Fawzy; El-Shamy, H.; Ghany, Abdel

doi:10.3989/gya.074413

Cited by 4 publications

(3 citation statements)

References 21 publications

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“…However, beyond this stage, we do not observe any significant changes in the LDls ( figure 2 b,c ), suggesting that metabolism of the LDs alters the LDl composition during early embryonic development. Table 2 gives the list of different lipid types and their corresponding R f values when TLC is run in neutral [ 35 ] and polar [ 36 ] solvent systems. Table 3 summarizes the LDl composition (as identified by TLC) at different developmental stages in control and OS-treated embryos.…”

Section: Resultsmentioning

confidence: 99%

Zebrafish lipid droplets regulate embryonic ATP homeostasis to power early development

Dutta¹,

Sinha²

2017

Open Biol.

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In zebrafish embryos, the maternally supplied pool of ATP is insufficient to power even the earliest of developmental events (0–3 hpf) such as oocyte-to-embryo transition (OET). The embryos generate an additional pulse (2.5 h long) of ATP (1.25–4 hpf) to achieve the embryonic ATP homeostasis. We demonstrate that the additional pulse of ATP is needed for successful execution of OET. The maternally supplied yolk lipids play a crucial role in maintaining the embryonic ATP homeostasis. In this paper, we identify the source and trafficking routes of free fatty acids (FFAs) that feed the mitochondria for synthesis of ATP. Interestingly, neither the maternally supplied pool of yolk-FFA nor the yolk-FACoA (fatty acyl coenzyme A) is used for ATP homeostasis during 0–5 hpf in zebrafish embryos. With the help of lipidomics, we explore the link between lipid droplet (LD)-mediated lipolysis and ATP homeostasis in zebrafish embryos. Until 5 hpf, the embryonic LDs undergo extensive lipolysis that generates FFAs. We demonstrate that these newly synthesized FFAs from LDs are involved in the maintenance of embryonic ATP homeostasis, rather than the FFAs/FACoA present in the yolk. Thus, the LDs are vital embryonic organelles that maintain the ATP homeostasis during early developmental stages (0–5 hpf) in zebrafish embryos. Our study highlights the important roles carried on by the LDs during the early development of the zebrafish embryos.

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Section: Resultsmentioning

confidence: 99%

Zebrafish lipid droplets regulate embryonic ATP homeostasis to power early development

Dutta¹,

Sinha²

2017

Open Biol.

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“…A plant known as Antirrhinum majus of the same family had shown antibacterial and antifungal activities because of the various compounds present in it, namely, lypipnin, lutein, chrysoeriol, kaempferol-3-gluscoside, and kaempferol-3,7-diglucoside. All of these compounds were flavonoids in nature [29]. Similarly, another compound known as aurone, isolated from this plant, has demonstrated antibacterial and antifungal activities.…”

Section: Antibacterial Activitymentioning

confidence: 92%

Antimicrobial, Cytotoxic, and Antioxidant Potential of a Novel Flavone “6,7,4′‐Trimethyl Flavone” Isolated from Wulfenia amherstiana

Kakar

Amin

Alghamdi

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Wulfenia amherstiana belongs to the Scrophulariaceae family and various plants of this family are known for their biological activities. The present study was focused on the isolation of bioactive compounds including a novel flavone 6,7,4′-trimethyl flavone (TMF) along with three known flavonoids such as quercetin, rutin, and a steroid β-sitosterol which were isolated from the ethanolic extract of W. amherstiana (Himalayan Wulfenia) through column chromatography and purified by using HPLC. Their structures were identified and elucidated through electron ionization mass spectroscopy (EIMS), 1DNMR (1H-NMR and 13C-NMR), and 2DNMR (COSY, HMQC, and HMBC) spectroscopy. The antimicrobial activities of this novel compound were evaluated through agar well diffusion method, while antioxidant and cytotoxic activities were assessed through 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging assay and brine shrimp lethality assay, respectively. The NMR data revealed that TMF is a novel compound. TMF showed potential antibacterial and antifungal activities against Staphylococcus aureus (MIC = 128 μg/ml) and Candida albicans (MIC = 128 μg/ml). The cytotoxic potential of TMF was determined from brine shrimp lethality assay with LD50 of 127.01 μg/ml. The free-radical scavenging potential of TMF at various concentrations implicated its strong antioxidant activity in vitro. The results revealed that TMF demonstrated substantial antimicrobial activity against S. aureus and C. albicans, strong antioxidant activity, and moderately cytotoxic activity.

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“…ϒ-tocopherol is the major tocopherol present in soybean oil with the sigma, alpha and beta compounds present in decreasing quantities; 62.8, 26.7, 9.3 and 1.2%, respectively (Gunstone et al, 2002). Values of 42.5% β-tocopherol, 41.3% γ-tocopherol, 15.9% α-tocopherol and 0.3% δ-tocopherol were found in canterbury bell (Campanula medium) seed oil (Hassaniena et al, 2014). The antioxidant properties of tocopherols may be involved in combating atherosclerosis by preventing the oxidation of low-density lipoproteins.…”

Section: Tocopherol Compositionmentioning

confidence: 99%

Physicochemical and antioxidant characteristics of gingerbread plum (<em>Neocarya macrophylla</em>) kernel oils

Diaby¹,

Amza²,

Onivogui³

et al. 2016

Grasas y Aceites

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SUMMARY:This study examined the physicochemical characteristics and antioxidant activities of oils extracted from gingerbread plum kernels grown in two different areas (Niger and Guinea). The oil contents were found to be significantly different (P≤0.05); 56% and 60% for gingerbread plum kernels originated from Niger (GPKN) and guinea (GPKG), respectively. GPKG showed the highest levels for physical parameters such as iodine, saponification, free fatty acid, acid and peroxide values when compared with GPKN. The major monounsaturated fatty acid in both GPKN and GPKG was oleic acid (42.46 and 41.43%, respectively) while the polyunsaturated fatty acids consisted of linoleic and arachidonic acid. Arachidonic acid was at higher levels (17.67 and 21.72% in GPKN and GPKG, respectively) than those in common vegetable oils. Results from antioxidant activity essays showed that GPKG is more active than GPKN in DPPH radical scavenging, β-carotene and phenolic contents while GPKN showed the highest values for reducing power and flavonoid. Of the 11 sterol compounds found in this study, 24-hydroxy-24-methyl cholesterol, clerosterol and sitosterol accounted for 68.5% and 66.33% in GPKN and GPKG, respectively. Finally, all tocopherol vitamers (except γ-tocopherol) were present in GPKN and GPKG with α-tocopherol being the main element in both samples. KEYWORDS: Antioxidant activity; Fatty acid profile; Gingerbread plum kernel; Oil indices; Sterols; Tocopherols RESUMEN:Características físico-químicas y antioxidantes del aceite de semillas de Neocarya macrophylla. En este estudio se examinaron las características físico-químicas y actividad antioxidante de los aceites de semilla de Neocarya macrophylla procedentes de dos regiones distintas, Níger y Guinea. Los contenidos grasos de las dos semillas resultaron ser significativamente (P≤0.05) diferentes: 56% para las semillas procedentes de Níger (GPKN) y 60% para las de Guinea (GPKG). El aceite de semillas de Guinea mostraba valores de parámetros químicos tales como índices de iodo, saponificación, peróxidos, y acidez, más elevados que el aceite de semillas de Níger. El ácido oleico resultó ser el ácido graso mono-insaturado más abundante en ambos casos (42.26% para el aceite de Níger y 41.42% para el de Guinea), mientras que los ácidos grasos poli-insaturados predominantes resultaron ser los ácidos linoleico y araquidódico. Los niveles de ácido araquidónico (17.68% para GPKN y 21.72% para GPKG) resultaron más elevados que los encontrados en otros aceites vegetales comunes. El aceite de GPKG demostró mayor capacidad de eliminación de radicales (ensayo de la difenil picrilhidrazina), así como mayores contenidos de β-caroteno y fenoles, mientras que el de GPKN mostró mayor poder reductor y contenido de flavonoides. De los 11 esteroles detectados, 24-hidroxi-24-metil colesterol, colesterol y sitosterol representaban el 68.5% y el 66.33% para los aceites de GPKN y GPKG respectivamente. Por último, todos los tocoferoles (excepto γ-tocoferol) estaban presentes en los dos aceites, siendo α...

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Characterization of fatty acids, bioactive lipids, and radical scavenging activity of Canterbury bells seed oil

Cited by 4 publications

References 21 publications

Zebrafish lipid droplets regulate embryonic ATP homeostasis to power early development

Zebrafish lipid droplets regulate embryonic ATP homeostasis to power early development

Antimicrobial, Cytotoxic, and Antioxidant Potential of a Novel Flavone “6,7,4′‐Trimethyl Flavone” Isolated from Wulfenia amherstiana

Physicochemical and antioxidant characteristics of gingerbread plum (<em>Neocarya macrophylla</em>) kernel oils

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